B-Cell Activation Biomarkers in Salivary Glands Are Related to Lymphomagenesis in Primary Sjögren’s Disease: A Pilot Monocentric Exploratory Study
Primary Sjögren’s disease is primarily driven by B-cell activation and is associated with a high risk of developing non-Hodgkin’s lymphoma (NHL). Over the last few decades, microRNA-155 (miR-155) has arisen as a key regulator of B-cells. Nevertheless, its role in primary Sjögren’s disease remains el...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/6/3259 |
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| author | Dario Bruno Barbara Tolusso Gianmarco Lugli Clara Di Mario Luca Petricca Simone Perniola Laura Bui Roberta Benvenuto Gianfranco Ferraccioli Stefano Alivernini Elisa Gremese |
| author_facet | Dario Bruno Barbara Tolusso Gianmarco Lugli Clara Di Mario Luca Petricca Simone Perniola Laura Bui Roberta Benvenuto Gianfranco Ferraccioli Stefano Alivernini Elisa Gremese |
| author_sort | Dario Bruno |
| collection | DOAJ |
| description | Primary Sjögren’s disease is primarily driven by B-cell activation and is associated with a high risk of developing non-Hodgkin’s lymphoma (NHL). Over the last few decades, microRNA-155 (miR-155) has arisen as a key regulator of B-cells. Nevertheless, its role in primary Sjögren’s disease remains elusive. Thus, the purpose of this study was (i) to explore miR-155, B-cell activating factor (BAFF)-receptor (BAFF-R), and Interleukin 6 receptor (IL-6R) expression in the labial salivary glands (LSG) of patients with primary Sjögren’s disease, aiming to identify potential B-cell activation biomarkers related to NHL development. Twenty-four patients with primary Sjögren’s disease, and with available tissue blocks from a LSG biopsy performed at diagnosis, were enrolled. Among them, five patients developed B-cell NHL during follow-up (7.3 ± 3.1 years). A comparison group of 20 individuals with sicca disease was included. Clinical and laboratory parameters were recorded and the LSG biopsies were evaluated to assess local inflammation in terms of miR-155/BAFF-R and IL-6R expression. Stratifying the primary Sjögren’s disease cohort according to lymphomagenesis, miR-155 was upregulated in primary Sjögren’s disease patients who experienced NHL, more so than those who did not experience NHL. Moreover, miR-155 expression correlated with the focus score (FS), as well as BAFF-R and IL-6R expression, which were increased in primary Sjögren’s disease patients and in turn related to neoplastic evolution. In conclusion, epigenetic modulation may play a crucial role in the aberrant activation of B-cells in primary Sjögren’s disease, profoundly impacting the risk of NHL development. |
| first_indexed | 2024-04-24T18:11:07Z |
| format | Article |
| id | doaj.art-3318819503d74c07bd9baaba219a55a5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T18:11:07Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3318819503d74c07bd9baaba219a55a52024-03-27T13:45:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01256325910.3390/ijms25063259B-Cell Activation Biomarkers in Salivary Glands Are Related to Lymphomagenesis in Primary Sjögren’s Disease: A Pilot Monocentric Exploratory StudyDario Bruno0Barbara Tolusso1Gianmarco Lugli2Clara Di Mario3Luca Petricca4Simone Perniola5Laura Bui6Roberta Benvenuto7Gianfranco Ferraccioli8Stefano Alivernini9Elisa Gremese10Clinical Immunology Unit, Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, ItalyImmunology Core Facility, Gemelli Science and Technology Park, Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, ItalyRare Disease Unit, Meyer Children’s Hospital IRCCS, 50139 Florence, ItalyImmunology Core Facility, Gemelli Science and Technology Park, Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, ItalyRheumatology Division, Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, ItalyClinical Immunology Unit, Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, ItalyInstitute of Pathology, Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, ItalyInstitute of Pathology, Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, ItalyDepartment of Internal Medicine, Catholic University of the Sacred Heart, 00168 Rome, ItalyImmunology Core Facility, Gemelli Science and Technology Park, Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, ItalyClinical Immunology Unit, Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, ItalyPrimary Sjögren’s disease is primarily driven by B-cell activation and is associated with a high risk of developing non-Hodgkin’s lymphoma (NHL). Over the last few decades, microRNA-155 (miR-155) has arisen as a key regulator of B-cells. Nevertheless, its role in primary Sjögren’s disease remains elusive. Thus, the purpose of this study was (i) to explore miR-155, B-cell activating factor (BAFF)-receptor (BAFF-R), and Interleukin 6 receptor (IL-6R) expression in the labial salivary glands (LSG) of patients with primary Sjögren’s disease, aiming to identify potential B-cell activation biomarkers related to NHL development. Twenty-four patients with primary Sjögren’s disease, and with available tissue blocks from a LSG biopsy performed at diagnosis, were enrolled. Among them, five patients developed B-cell NHL during follow-up (7.3 ± 3.1 years). A comparison group of 20 individuals with sicca disease was included. Clinical and laboratory parameters were recorded and the LSG biopsies were evaluated to assess local inflammation in terms of miR-155/BAFF-R and IL-6R expression. Stratifying the primary Sjögren’s disease cohort according to lymphomagenesis, miR-155 was upregulated in primary Sjögren’s disease patients who experienced NHL, more so than those who did not experience NHL. Moreover, miR-155 expression correlated with the focus score (FS), as well as BAFF-R and IL-6R expression, which were increased in primary Sjögren’s disease patients and in turn related to neoplastic evolution. In conclusion, epigenetic modulation may play a crucial role in the aberrant activation of B-cells in primary Sjögren’s disease, profoundly impacting the risk of NHL development.https://www.mdpi.com/1422-0067/25/6/3259primary Sjögren’s disease (primary Sjögren’s disease)lymphomagenesismicroRNA-155BAFF-receptorIL-6 receptorbiomarkers |
| spellingShingle | Dario Bruno Barbara Tolusso Gianmarco Lugli Clara Di Mario Luca Petricca Simone Perniola Laura Bui Roberta Benvenuto Gianfranco Ferraccioli Stefano Alivernini Elisa Gremese B-Cell Activation Biomarkers in Salivary Glands Are Related to Lymphomagenesis in Primary Sjögren’s Disease: A Pilot Monocentric Exploratory Study International Journal of Molecular Sciences primary Sjögren’s disease (primary Sjögren’s disease) lymphomagenesis microRNA-155 BAFF-receptor IL-6 receptor biomarkers |
| title | B-Cell Activation Biomarkers in Salivary Glands Are Related to Lymphomagenesis in Primary Sjögren’s Disease: A Pilot Monocentric Exploratory Study |
| title_full | B-Cell Activation Biomarkers in Salivary Glands Are Related to Lymphomagenesis in Primary Sjögren’s Disease: A Pilot Monocentric Exploratory Study |
| title_fullStr | B-Cell Activation Biomarkers in Salivary Glands Are Related to Lymphomagenesis in Primary Sjögren’s Disease: A Pilot Monocentric Exploratory Study |
| title_full_unstemmed | B-Cell Activation Biomarkers in Salivary Glands Are Related to Lymphomagenesis in Primary Sjögren’s Disease: A Pilot Monocentric Exploratory Study |
| title_short | B-Cell Activation Biomarkers in Salivary Glands Are Related to Lymphomagenesis in Primary Sjögren’s Disease: A Pilot Monocentric Exploratory Study |
| title_sort | b cell activation biomarkers in salivary glands are related to lymphomagenesis in primary sjogren s disease a pilot monocentric exploratory study |
| topic | primary Sjögren’s disease (primary Sjögren’s disease) lymphomagenesis microRNA-155 BAFF-receptor IL-6 receptor biomarkers |
| url | https://www.mdpi.com/1422-0067/25/6/3259 |
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