The Expression of Limonene Biosynthesis Pathway Key Genes under Drought Stress in Dracocephalum kotschyi at Vegetative and Flowering Stages
<strong>Objective</strong> <em>Dracocephalum kotschyi</em> is an endemic medicinal plant in Iran belonging to the Lamiaceae family. One of the most important monoterpenes identified in this plant is limonene. Drought stress is one of the factors affecting the content of mono...
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
2019-11-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_2472_2031fd9810e80f482bcb104790823e7e.pdf |
| _version_ | 1818524030264672256 |
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| author | Soghra Kiani Jafar Ahmadi behroz shiran sedigheh fabriki ourang Hossein Fallahi |
| author_facet | Soghra Kiani Jafar Ahmadi behroz shiran sedigheh fabriki ourang Hossein Fallahi |
| author_sort | Soghra Kiani |
| collection | DOAJ |
| description | <strong>Objective</strong>
<em>Dracocephalum kotschyi</em> is an endemic medicinal plant in Iran belonging to the Lamiaceae family. One of the most important monoterpenes identified in this plant is limonene. Drought stress is one of the factors affecting the content of monoterpene in medicinal plants. In this study, the effect of different levels of drought stress was investigated on the expression of some genes involved in limonene biosynthetic pathway in <em>D. kotschyi</em> at two growth stages.
<strong>Materials and Methods</strong>
This experiment was conducted as a factorial experiment with two factor included drought stress and growth stages in a completely randomized design with three replications. Drought stress consisted of severe (25% FC), moderate (50% FC), low (75% FC) stress and non-stress or control (100% FC) and growth stages consisted of vegetative and flowering. Secondary metabolites were analyzed and the amount of limonene was measured by GC / MS using Head-space method and the relative expression of key genes involved in limonene biosynthesis was measured using Real Time PCR.
<strong>Results</strong>
<strong> </strong>Analysis of secondary metabolites showed, one of the main monoterpenes was limonene whose amounts was 20.57 % and 13.41% during vegetative and flowering stages, respectively. Drought stress significantly increased <em>HMGR</em> and <em>DXS</em> gene expression during flowering stage, while during vegetative stage no significant change was observed on expression of both genes. In both growth stages, low drought stress increased <em>HDR</em> gene expression, although at flowering stage this increase was not significant but severe drought decreased <em>HDR</em> gene expression, although this decrease was not significant at vegetative stage. Drought stress decreased <em>LS</em> gene expression during flowering stage and increased it during vegetative stage.
<strong>Conclusion</strong>
The results indicated that limonene content and expression of limonene biosynthesis key genes in <em>D</em>. <em>kotschyi</em> under drought stress are growth stage-specific. |
| first_indexed | 2024-12-11T05:52:08Z |
| format | Article |
| id | doaj.art-331da794c61e49d0949583aba34cdf59 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-11T05:52:08Z |
| publishDate | 2019-11-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-331da794c61e49d0949583aba34cdf592022-12-22T01:18:47ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002019-11-0111311810.22103/jab.2019.24722472The Expression of Limonene Biosynthesis Pathway Key Genes under Drought Stress in Dracocephalum kotschyi at Vegetative and Flowering StagesSoghra Kiani0Jafar Ahmadi1behroz shiran2sedigheh fabriki ourang3Hossein Fallahi4payame noor university, faculty of agriculture, iran, shahrekord, farokhsharProfessor, Department of Genetics and Plant Breeding, Faculty of Agriculture, Imam Khomeini International University, Qazvin, Iran.Professor of Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekord, IranAssociate Professor, Department of Genetics and Plant Breeding, Faculty of Agriculture, Imam Khomeini International University, Qazvin, IranAssistant Professor, Department of Biology, School of Sciences, Razi University, Bagh-e-Abrisham Kermanshah, Iran<strong>Objective</strong> <em>Dracocephalum kotschyi</em> is an endemic medicinal plant in Iran belonging to the Lamiaceae family. One of the most important monoterpenes identified in this plant is limonene. Drought stress is one of the factors affecting the content of monoterpene in medicinal plants. In this study, the effect of different levels of drought stress was investigated on the expression of some genes involved in limonene biosynthetic pathway in <em>D. kotschyi</em> at two growth stages. <strong>Materials and Methods</strong> This experiment was conducted as a factorial experiment with two factor included drought stress and growth stages in a completely randomized design with three replications. Drought stress consisted of severe (25% FC), moderate (50% FC), low (75% FC) stress and non-stress or control (100% FC) and growth stages consisted of vegetative and flowering. Secondary metabolites were analyzed and the amount of limonene was measured by GC / MS using Head-space method and the relative expression of key genes involved in limonene biosynthesis was measured using Real Time PCR. <strong>Results</strong> <strong> </strong>Analysis of secondary metabolites showed, one of the main monoterpenes was limonene whose amounts was 20.57 % and 13.41% during vegetative and flowering stages, respectively. Drought stress significantly increased <em>HMGR</em> and <em>DXS</em> gene expression during flowering stage, while during vegetative stage no significant change was observed on expression of both genes. In both growth stages, low drought stress increased <em>HDR</em> gene expression, although at flowering stage this increase was not significant but severe drought decreased <em>HDR</em> gene expression, although this decrease was not significant at vegetative stage. Drought stress decreased <em>LS</em> gene expression during flowering stage and increased it during vegetative stage. <strong>Conclusion</strong> The results indicated that limonene content and expression of limonene biosynthesis key genes in <em>D</em>. <em>kotschyi</em> under drought stress are growth stage-specific.https://jab.uk.ac.ir/article_2472_2031fd9810e80f482bcb104790823e7e.pdfdracocephalum kotschyidrought stressdxshmgrls |
| spellingShingle | Soghra Kiani Jafar Ahmadi behroz shiran sedigheh fabriki ourang Hossein Fallahi The Expression of Limonene Biosynthesis Pathway Key Genes under Drought Stress in Dracocephalum kotschyi at Vegetative and Flowering Stages مجله بیوتکنولوژی کشاورزی dracocephalum kotschyi drought stress dxs hmgr ls |
| title | The Expression of Limonene Biosynthesis Pathway Key Genes under Drought Stress in Dracocephalum kotschyi at Vegetative and Flowering Stages |
| title_full | The Expression of Limonene Biosynthesis Pathway Key Genes under Drought Stress in Dracocephalum kotschyi at Vegetative and Flowering Stages |
| title_fullStr | The Expression of Limonene Biosynthesis Pathway Key Genes under Drought Stress in Dracocephalum kotschyi at Vegetative and Flowering Stages |
| title_full_unstemmed | The Expression of Limonene Biosynthesis Pathway Key Genes under Drought Stress in Dracocephalum kotschyi at Vegetative and Flowering Stages |
| title_short | The Expression of Limonene Biosynthesis Pathway Key Genes under Drought Stress in Dracocephalum kotschyi at Vegetative and Flowering Stages |
| title_sort | expression of limonene biosynthesis pathway key genes under drought stress in dracocephalum kotschyi at vegetative and flowering stages |
| topic | dracocephalum kotschyi drought stress dxs hmgr ls |
| url | https://jab.uk.ac.ir/article_2472_2031fd9810e80f482bcb104790823e7e.pdf |
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