RsGSTF12 Contributes to Anthocyanin Sequestration in Radish (Raphanus sativus L.)
Anthocyanins are water-soluble plant pigments mainly stored in the plant vacuoles. Glutathione S-transferases (GSTs) are a multifunctional enzyme family, which can regulate substance metabolism and biological and abiotic stresses in plants. However, few reports were focused on the involvement of GST...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.870202/full |
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| author | Mengyang Niu Changjian Bao Jiahui Chen Wen Zhou Yueyue Zhang Xiaoyan Zhang Nana Su Jin Cui |
| author_facet | Mengyang Niu Changjian Bao Jiahui Chen Wen Zhou Yueyue Zhang Xiaoyan Zhang Nana Su Jin Cui |
| author_sort | Mengyang Niu |
| collection | DOAJ |
| description | Anthocyanins are water-soluble plant pigments mainly stored in the plant vacuoles. Glutathione S-transferases (GSTs) are a multifunctional enzyme family, which can regulate substance metabolism and biological and abiotic stresses in plants. However, few reports were focused on the involvement of GSTs in anthocyanin sequestration in red skin radish. Here, we identified a glutathione S-transferase gene RsGSTF12 that played roles in anthocyanin sequestration in radish. The bioinformatics analysis revealed that RsGSTF12 belonged to the phi (F) class of glutathione S-transferases and showed a high homology with AtGSTF12, followed by AtGSTF11. The subcellular localization assay showed that RsGSTF12 was located in the endoplasmic reticulum and tonoplast. Temporal and spatial gene expression-specific analyses uncovered a strong correlation of RsGSTF12 with anthocyanin accumulation in radish sprouts. The anthocyanin solubility assay found RsGSTF12 was capable of improving cyanidin water solubility in vitro. Transiently expressing RsGSTF12 in radish cotyledons was able to increase their anthocyanin sequestrations. Furthermore, the functional complementation and overexpression of the Arabidopsis thaliana tt19 mutant and wild type demonstrated that RsGSTF12 might play an indispensable role in anthocyanin accumulation in radish. Taken together, we provide compelling evidence that RsGSTF12 functions critically in how anthocyanins are sequestrated in radish, which may enrich our understanding of the mechanism of anthocyanin sequestration. |
| first_indexed | 2024-12-11T18:43:15Z |
| format | Article |
| id | doaj.art-331df71580a24da2a20ba975f6af99c4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T18:43:15Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-331df71580a24da2a20ba975f6af99c42022-12-22T00:54:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.870202870202RsGSTF12 Contributes to Anthocyanin Sequestration in Radish (Raphanus sativus L.)Mengyang NiuChangjian BaoJiahui ChenWen ZhouYueyue ZhangXiaoyan ZhangNana SuJin CuiAnthocyanins are water-soluble plant pigments mainly stored in the plant vacuoles. Glutathione S-transferases (GSTs) are a multifunctional enzyme family, which can regulate substance metabolism and biological and abiotic stresses in plants. However, few reports were focused on the involvement of GSTs in anthocyanin sequestration in red skin radish. Here, we identified a glutathione S-transferase gene RsGSTF12 that played roles in anthocyanin sequestration in radish. The bioinformatics analysis revealed that RsGSTF12 belonged to the phi (F) class of glutathione S-transferases and showed a high homology with AtGSTF12, followed by AtGSTF11. The subcellular localization assay showed that RsGSTF12 was located in the endoplasmic reticulum and tonoplast. Temporal and spatial gene expression-specific analyses uncovered a strong correlation of RsGSTF12 with anthocyanin accumulation in radish sprouts. The anthocyanin solubility assay found RsGSTF12 was capable of improving cyanidin water solubility in vitro. Transiently expressing RsGSTF12 in radish cotyledons was able to increase their anthocyanin sequestrations. Furthermore, the functional complementation and overexpression of the Arabidopsis thaliana tt19 mutant and wild type demonstrated that RsGSTF12 might play an indispensable role in anthocyanin accumulation in radish. Taken together, we provide compelling evidence that RsGSTF12 functions critically in how anthocyanins are sequestrated in radish, which may enrich our understanding of the mechanism of anthocyanin sequestration.https://www.frontiersin.org/articles/10.3389/fpls.2022.870202/fullradishanthocyaninglutathione S-transferasevacuolesequestration |
| spellingShingle | Mengyang Niu Changjian Bao Jiahui Chen Wen Zhou Yueyue Zhang Xiaoyan Zhang Nana Su Jin Cui RsGSTF12 Contributes to Anthocyanin Sequestration in Radish (Raphanus sativus L.) Frontiers in Plant Science radish anthocyanin glutathione S-transferase vacuole sequestration |
| title | RsGSTF12 Contributes to Anthocyanin Sequestration in Radish (Raphanus sativus L.) |
| title_full | RsGSTF12 Contributes to Anthocyanin Sequestration in Radish (Raphanus sativus L.) |
| title_fullStr | RsGSTF12 Contributes to Anthocyanin Sequestration in Radish (Raphanus sativus L.) |
| title_full_unstemmed | RsGSTF12 Contributes to Anthocyanin Sequestration in Radish (Raphanus sativus L.) |
| title_short | RsGSTF12 Contributes to Anthocyanin Sequestration in Radish (Raphanus sativus L.) |
| title_sort | rsgstf12 contributes to anthocyanin sequestration in radish raphanus sativus l |
| topic | radish anthocyanin glutathione S-transferase vacuole sequestration |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.870202/full |
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