Controllable and reusable seesaw circuit based on nicking endonucleases
Abstract Seesaw circuits are essential for molecular computing and biosensing. However, a notable limitation of seesaw circuits lies in the irreversible depletion of components, precluding the attainment of system recovery and rendering nucleic acid circuits non-reusable. We developed a brand-new me...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-04-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-024-02388-6 |
| _version_ | 1797219582161715200 |
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| author | Yuheng Liao Yizhou Liu Huan Liu Xiao Liu Longjie Li Xianjin Xiao |
| author_facet | Yuheng Liao Yizhou Liu Huan Liu Xiao Liu Longjie Li Xianjin Xiao |
| author_sort | Yuheng Liao |
| collection | DOAJ |
| description | Abstract Seesaw circuits are essential for molecular computing and biosensing. However, a notable limitation of seesaw circuits lies in the irreversible depletion of components, precluding the attainment of system recovery and rendering nucleic acid circuits non-reusable. We developed a brand-new method for creating controllable and reusable seesaw circuits. By using the nicking endonucleases Nt.BbvCI and Nt.Alwi, we removed “functional components” while keeping the “skeletal components” for recurrent usage. T-inputs were introduced, increasing the signal-to-noise ratio of AND logic from 2.68 to 11.33 and demonstrating compatibility. We identified the logic switching feature and verified that it does not impair circuit performance. We also built intricate logic circuits, such as OR-AND gate, to demonstrate the versatility of our methodology. This controllable reusability extends the applications of nanotechnology and bioengineering, enhancing the practicality and efficiency of these circuits across various domains. |
| first_indexed | 2024-04-24T12:35:56Z |
| format | Article |
| id | doaj.art-331f0d7d306d426ba2a09a5690c21a73 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-24T12:35:56Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-331f0d7d306d426ba2a09a5690c21a732024-04-07T11:29:16ZengBMCJournal of Nanobiotechnology1477-31552024-04-0122111410.1186/s12951-024-02388-6Controllable and reusable seesaw circuit based on nicking endonucleasesYuheng Liao0Yizhou Liu1Huan Liu2Xiao Liu3Longjie Li4Xianjin Xiao5Insititute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInsititute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInsititute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInsititute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologySchool of Life Science and Technology, Wuhan Polytechnic UniversityInsititute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Seesaw circuits are essential for molecular computing and biosensing. However, a notable limitation of seesaw circuits lies in the irreversible depletion of components, precluding the attainment of system recovery and rendering nucleic acid circuits non-reusable. We developed a brand-new method for creating controllable and reusable seesaw circuits. By using the nicking endonucleases Nt.BbvCI and Nt.Alwi, we removed “functional components” while keeping the “skeletal components” for recurrent usage. T-inputs were introduced, increasing the signal-to-noise ratio of AND logic from 2.68 to 11.33 and demonstrating compatibility. We identified the logic switching feature and verified that it does not impair circuit performance. We also built intricate logic circuits, such as OR-AND gate, to demonstrate the versatility of our methodology. This controllable reusability extends the applications of nanotechnology and bioengineering, enhancing the practicality and efficiency of these circuits across various domains.https://doi.org/10.1186/s12951-024-02388-6DNASeesaw circuitLogic gateReusableControllable |
| spellingShingle | Yuheng Liao Yizhou Liu Huan Liu Xiao Liu Longjie Li Xianjin Xiao Controllable and reusable seesaw circuit based on nicking endonucleases Journal of Nanobiotechnology DNA Seesaw circuit Logic gate Reusable Controllable |
| title | Controllable and reusable seesaw circuit based on nicking endonucleases |
| title_full | Controllable and reusable seesaw circuit based on nicking endonucleases |
| title_fullStr | Controllable and reusable seesaw circuit based on nicking endonucleases |
| title_full_unstemmed | Controllable and reusable seesaw circuit based on nicking endonucleases |
| title_short | Controllable and reusable seesaw circuit based on nicking endonucleases |
| title_sort | controllable and reusable seesaw circuit based on nicking endonucleases |
| topic | DNA Seesaw circuit Logic gate Reusable Controllable |
| url | https://doi.org/10.1186/s12951-024-02388-6 |
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