Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids
Abstract Fluorescence imaging of nucleic acids is a very important technique necessary to understand gene expression and the resulting changes in cell function. This mini-review focuses on sequence-specific fluorescence imaging of intracellular RNA and methylated DNA using fluorescent nucleic acid p...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-11-01
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| Series: | Applied Microscopy |
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| Online Access: | http://link.springer.com/article/10.1186/s42649-019-0017-1 |
| _version_ | 1828812534327541760 |
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| author | Akimitsu Okamoto |
| author_facet | Akimitsu Okamoto |
| author_sort | Akimitsu Okamoto |
| collection | DOAJ |
| description | Abstract Fluorescence imaging of nucleic acids is a very important technique necessary to understand gene expression and the resulting changes in cell function. This mini-review focuses on sequence-specific fluorescence imaging of intracellular RNA and methylated DNA using fluorescent nucleic acid probes. A couple of functional fluorescent nucleic acid probes developed by our laboratory are introduced and the examples of their application to fluorescence imaging of intracellular nucleic acids are described. |
| first_indexed | 2024-12-12T09:45:39Z |
| format | Article |
| id | doaj.art-33274f9424c64ee29e8bb5eafe692b09 |
| institution | Directory Open Access Journal |
| issn | 2287-4445 |
| language | English |
| last_indexed | 2024-12-12T09:45:39Z |
| publishDate | 2019-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Microscopy |
| spelling | doaj.art-33274f9424c64ee29e8bb5eafe692b092022-12-22T00:28:25ZengSpringerOpenApplied Microscopy2287-44452019-11-014911710.1186/s42649-019-0017-1Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acidsAkimitsu Okamoto0Research Center for Advanced Science and Technology, The University of TokyoAbstract Fluorescence imaging of nucleic acids is a very important technique necessary to understand gene expression and the resulting changes in cell function. This mini-review focuses on sequence-specific fluorescence imaging of intracellular RNA and methylated DNA using fluorescent nucleic acid probes. A couple of functional fluorescent nucleic acid probes developed by our laboratory are introduced and the examples of their application to fluorescence imaging of intracellular nucleic acids are described.http://link.springer.com/article/10.1186/s42649-019-0017-1Methylated DNARNAFluorescenceSingle cell imagingSynthetic nucleic acid |
| spellingShingle | Akimitsu Okamoto Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids Applied Microscopy Methylated DNA RNA Fluorescence Single cell imaging Synthetic nucleic acid |
| title | Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids |
| title_full | Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids |
| title_fullStr | Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids |
| title_full_unstemmed | Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids |
| title_short | Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids |
| title_sort | next generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids |
| topic | Methylated DNA RNA Fluorescence Single cell imaging Synthetic nucleic acid |
| url | http://link.springer.com/article/10.1186/s42649-019-0017-1 |
| work_keys_str_mv | AT akimitsuokamoto nextgenerationfluorescentnucleicacidsprobesformicroscopicanalysisofintracellularnucleicacids |