Prevalence of Toxoplasma gondii and Neospora caninum contaminations in poultry eggs: molecular surveillance in three different geographical regions of Iran
Abstract Background Toxoplasma gondii and Neospora caninum are important protozoan parasites with worldwide distribution among warm-blooded animals. Moreover, T. gondii is a zoonotic parasite that infects humans. Poultries are important intermediated hosts of T. gondii and N. caninum. However, littl...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Food Safety and Risk |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40550-023-00103-1 |
| _version_ | 1797774009187696640 |
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| author | Salar Maani Kavous Solhjoo Mohammad Aref Bagherzadeh Ahmadreza Bazmjoo Hooshmand Ghorbaani Barnaaji Hamed Mir Fares Bahrami Seyedeh Zahra Khademi Morteza Shams Amir Abdoli Fatemeh Ghaffarifar |
| author_facet | Salar Maani Kavous Solhjoo Mohammad Aref Bagherzadeh Ahmadreza Bazmjoo Hooshmand Ghorbaani Barnaaji Hamed Mir Fares Bahrami Seyedeh Zahra Khademi Morteza Shams Amir Abdoli Fatemeh Ghaffarifar |
| author_sort | Salar Maani |
| collection | DOAJ |
| description | Abstract Background Toxoplasma gondii and Neospora caninum are important protozoan parasites with worldwide distribution among warm-blooded animals. Moreover, T. gondii is a zoonotic parasite that infects humans. Poultries are important intermediated hosts of T. gondii and N. caninum. However, little is known about the contamination of poultry eggs with these parasites. We aimed to investigate the molecular frequency of T. gondii and N. caninum among the eggs of chicken, domestic duck, and quail from three different geographic regions of Iran. T. gondii and N. caninum were detected by PCR targeting the RE and Nc5 genes, respectively. Findings Overall contamination rates with T. gondii and N. caninum were 10.7 and 5.9%, respectively. The overall contamination rates of T. gondii among chicken, duck, and quail were 12.2, 15.5, and 4.4%, respectively; while N. caninum was detected in 11.1, 3.3, and 1.1% of the same samples, respectively. The contamination rates were increased with increasing humidity across three different regions. Conclusions Taken together, this study indicates the contamination of poultry eggs with T. gondii and N. caninum. The possibility of egg-born transmission of T. gondii should not be neglected by consuming raw soft-boiled eggs. Furthermore, contamination of poultry eggs could be an indicator for environmental contamination by these parasites. |
| first_indexed | 2024-03-12T22:14:43Z |
| format | Article |
| id | doaj.art-332759c85b43413facd161df08291f91 |
| institution | Directory Open Access Journal |
| issn | 2731-9245 |
| language | English |
| last_indexed | 2024-03-12T22:14:43Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Food Safety and Risk |
| spelling | doaj.art-332759c85b43413facd161df08291f912023-07-23T11:21:54ZengBMCFood Safety and Risk2731-92452023-02-011011510.1186/s40550-023-00103-1Prevalence of Toxoplasma gondii and Neospora caninum contaminations in poultry eggs: molecular surveillance in three different geographical regions of IranSalar Maani0Kavous Solhjoo1Mohammad Aref Bagherzadeh2Ahmadreza Bazmjoo3Hooshmand Ghorbaani Barnaaji4Hamed Mir5Fares Bahrami6Seyedeh Zahra Khademi7Morteza Shams8Amir Abdoli9Fatemeh Ghaffarifar10Zoonoses Research Center, Jahrom University of Medical SciencesZoonoses Research Center, Jahrom University of Medical SciencesStudent Research Committee, Jahrom University of Medical SciencesStudent Research Committee, Jahrom University of Medical SciencesDepartment of Parasitology and Mycology, Jahrom University of Medical SciencesResearch Center for Noncommunicable Diseases, Jahrom University of Medical SciencesZoonoses Research Center, Research Institute for Health Development, Kurdistan University of Medical SciencesDepartment of Management, Payam Noor University (PNU)Zoonotic Diseases Research Center, Ilam University of Medical SciencesZoonoses Research Center, Jahrom University of Medical SciencesDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares UniversityAbstract Background Toxoplasma gondii and Neospora caninum are important protozoan parasites with worldwide distribution among warm-blooded animals. Moreover, T. gondii is a zoonotic parasite that infects humans. Poultries are important intermediated hosts of T. gondii and N. caninum. However, little is known about the contamination of poultry eggs with these parasites. We aimed to investigate the molecular frequency of T. gondii and N. caninum among the eggs of chicken, domestic duck, and quail from three different geographic regions of Iran. T. gondii and N. caninum were detected by PCR targeting the RE and Nc5 genes, respectively. Findings Overall contamination rates with T. gondii and N. caninum were 10.7 and 5.9%, respectively. The overall contamination rates of T. gondii among chicken, duck, and quail were 12.2, 15.5, and 4.4%, respectively; while N. caninum was detected in 11.1, 3.3, and 1.1% of the same samples, respectively. The contamination rates were increased with increasing humidity across three different regions. Conclusions Taken together, this study indicates the contamination of poultry eggs with T. gondii and N. caninum. The possibility of egg-born transmission of T. gondii should not be neglected by consuming raw soft-boiled eggs. Furthermore, contamination of poultry eggs could be an indicator for environmental contamination by these parasites.https://doi.org/10.1186/s40550-023-00103-1Toxoplasma gondiiNeospora caninumPoultryEggsHumidityChickens |
| spellingShingle | Salar Maani Kavous Solhjoo Mohammad Aref Bagherzadeh Ahmadreza Bazmjoo Hooshmand Ghorbaani Barnaaji Hamed Mir Fares Bahrami Seyedeh Zahra Khademi Morteza Shams Amir Abdoli Fatemeh Ghaffarifar Prevalence of Toxoplasma gondii and Neospora caninum contaminations in poultry eggs: molecular surveillance in three different geographical regions of Iran Food Safety and Risk Toxoplasma gondii Neospora caninum Poultry Eggs Humidity Chickens |
| title | Prevalence of Toxoplasma gondii and Neospora caninum contaminations in poultry eggs: molecular surveillance in three different geographical regions of Iran |
| title_full | Prevalence of Toxoplasma gondii and Neospora caninum contaminations in poultry eggs: molecular surveillance in three different geographical regions of Iran |
| title_fullStr | Prevalence of Toxoplasma gondii and Neospora caninum contaminations in poultry eggs: molecular surveillance in three different geographical regions of Iran |
| title_full_unstemmed | Prevalence of Toxoplasma gondii and Neospora caninum contaminations in poultry eggs: molecular surveillance in three different geographical regions of Iran |
| title_short | Prevalence of Toxoplasma gondii and Neospora caninum contaminations in poultry eggs: molecular surveillance in three different geographical regions of Iran |
| title_sort | prevalence of toxoplasma gondii and neospora caninum contaminations in poultry eggs molecular surveillance in three different geographical regions of iran |
| topic | Toxoplasma gondii Neospora caninum Poultry Eggs Humidity Chickens |
| url | https://doi.org/10.1186/s40550-023-00103-1 |
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