Determination of Factor II Codons Genotype in Southeastern Iranian Patients With Hereditary Deficiency of Factor II
Objectives: Congenital prothrombin (factor II) deficiency is an inherited rare bleeding disorder with an autosomal recessive manner. The prevalence of this disorder is about one in 2000000 people in general population, but it is more common in areas with a high rate of consanguinity. To date, there...
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| Format: | Article |
| Language: | English |
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Aras Part Medical International Press
2023-04-01
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| Series: | Crescent Journal of Medical and Biological Sciences |
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| Online Access: | http://www.cjmb.org/pdf.php?id=588 |
| _version_ | 1797830765710409728 |
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| author | Hamed Soleimani Samarkhazan Shaban Alizadeh Ziba Majidi Zahra Kashani Khatib Majid Naderi |
| author_facet | Hamed Soleimani Samarkhazan Shaban Alizadeh Ziba Majidi Zahra Kashani Khatib Majid Naderi |
| author_sort | Hamed Soleimani Samarkhazan |
| collection | DOAJ |
| description | Objectives: Congenital prothrombin (factor II) deficiency is an inherited rare bleeding disorder with an autosomal recessive manner. The prevalence of this disorder is about one in 2000000 people in general population, but it is more common in areas with a high rate of consanguinity. To date, there is no report on the absence of prothrombin, which is a life-threating disorder. Considering the importance of factor II in body homeostasis, this study aimed to find any possible mutation of coagulation factor II codons in patients with inherited factor II deficiency in southeastern Iran. Materials andMethods: This study was conducted on 12 patients with inherited deficiency of prothrombin. Early diagnosis was based on clinical symptoms, laboratory evaluation, and family history. Then, the function level of prothrombin was measured, the initial diagnosis of disease was confirmed, and polymerase chain reaction (PCR) analysis was performed. Finally, gene sequencing and genotyping of factor II was done. Results: Molecular analysis indicated a point mutation in exon 7 in three patients and a frameshift mutation in exon 14 due to addition of a thymine base at position 1760-1761 in one patient, both of which have been reported for the first time. Conclusions: Molecular methods performed on patients from Southeastern Iranian population in terms of coagulation factor II deficiency revealed a substitution mutation in exon 7 in three patients and a frameshift mutation in exon 14 in one patient, both of which were reported for the first time. Considering the significant difference between the clinical symptoms of the present study and previous studies, probably the type of mutations reported in this study (for the first time) caused these clinical symptoms, but statistical studies did not show any relationship between the type of mutation and the occurrence of clinical symptoms. And it needs more investigations on more patients, with a larger population. |
| first_indexed | 2024-04-09T13:42:25Z |
| format | Article |
| id | doaj.art-33285a18aa7c42d6bf7bf1a035e47206 |
| institution | Directory Open Access Journal |
| issn | 2148-9696 |
| language | English |
| last_indexed | 2024-04-09T13:42:25Z |
| publishDate | 2023-04-01 |
| publisher | Aras Part Medical International Press |
| record_format | Article |
| series | Crescent Journal of Medical and Biological Sciences |
| spelling | doaj.art-33285a18aa7c42d6bf7bf1a035e472062023-05-09T07:42:44ZengAras Part Medical International PressCrescent Journal of Medical and Biological Sciences2148-96962023-04-01102616610.34172/cjmb.2023.10CJMB-9075Determination of Factor II Codons Genotype in Southeastern Iranian Patients With Hereditary Deficiency of Factor IIHamed Soleimani Samarkhazan0Shaban Alizadeh1Ziba Majidi2Zahra Kashani Khatib3Majid Naderi4Department of Hematology and Transfusion Medicine, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Hematology and Transfusion Medicine, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Medical Laboratory Science, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.Laboratory Hematology and Blood Banking, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.Department of Hematology and Transfusion Medicine, School of Allied Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.Objectives: Congenital prothrombin (factor II) deficiency is an inherited rare bleeding disorder with an autosomal recessive manner. The prevalence of this disorder is about one in 2000000 people in general population, but it is more common in areas with a high rate of consanguinity. To date, there is no report on the absence of prothrombin, which is a life-threating disorder. Considering the importance of factor II in body homeostasis, this study aimed to find any possible mutation of coagulation factor II codons in patients with inherited factor II deficiency in southeastern Iran. Materials andMethods: This study was conducted on 12 patients with inherited deficiency of prothrombin. Early diagnosis was based on clinical symptoms, laboratory evaluation, and family history. Then, the function level of prothrombin was measured, the initial diagnosis of disease was confirmed, and polymerase chain reaction (PCR) analysis was performed. Finally, gene sequencing and genotyping of factor II was done. Results: Molecular analysis indicated a point mutation in exon 7 in three patients and a frameshift mutation in exon 14 due to addition of a thymine base at position 1760-1761 in one patient, both of which have been reported for the first time. Conclusions: Molecular methods performed on patients from Southeastern Iranian population in terms of coagulation factor II deficiency revealed a substitution mutation in exon 7 in three patients and a frameshift mutation in exon 14 in one patient, both of which were reported for the first time. Considering the significant difference between the clinical symptoms of the present study and previous studies, probably the type of mutations reported in this study (for the first time) caused these clinical symptoms, but statistical studies did not show any relationship between the type of mutation and the occurrence of clinical symptoms. And it needs more investigations on more patients, with a larger population.http://www.cjmb.org/pdf.php?id=588factor ii deficiencyprothrombincongenital bleeding disordersblood coagulation factor |
| spellingShingle | Hamed Soleimani Samarkhazan Shaban Alizadeh Ziba Majidi Zahra Kashani Khatib Majid Naderi Determination of Factor II Codons Genotype in Southeastern Iranian Patients With Hereditary Deficiency of Factor II Crescent Journal of Medical and Biological Sciences factor ii deficiency prothrombin congenital bleeding disorders blood coagulation factor |
| title | Determination of Factor II Codons Genotype in Southeastern Iranian Patients With Hereditary Deficiency of Factor II |
| title_full | Determination of Factor II Codons Genotype in Southeastern Iranian Patients With Hereditary Deficiency of Factor II |
| title_fullStr | Determination of Factor II Codons Genotype in Southeastern Iranian Patients With Hereditary Deficiency of Factor II |
| title_full_unstemmed | Determination of Factor II Codons Genotype in Southeastern Iranian Patients With Hereditary Deficiency of Factor II |
| title_short | Determination of Factor II Codons Genotype in Southeastern Iranian Patients With Hereditary Deficiency of Factor II |
| title_sort | determination of factor ii codons genotype in southeastern iranian patients with hereditary deficiency of factor ii |
| topic | factor ii deficiency prothrombin congenital bleeding disorders blood coagulation factor |
| url | http://www.cjmb.org/pdf.php?id=588 |
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