Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje
Background/Aim. Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains appear to have rapidly disseminated among population in the community without established risk factors for MRSA worldwide. Panton-Valentine leukocidin (PVL) is a cytolytic toxin, encoded by th...
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2016-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2016/0042-84501600003P.pdf |
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| author | Petrović-Jeremić Ljiljana Kuljić-Kapulica Nada Ristanović Elizabeta Jošić Dragana Lepšanović Zorica |
| author_facet | Petrović-Jeremić Ljiljana Kuljić-Kapulica Nada Ristanović Elizabeta Jošić Dragana Lepšanović Zorica |
| author_sort | Petrović-Jeremić Ljiljana |
| collection | DOAJ |
| description | Background/Aim. Community-associated methicillin-resistant Staphylococcus
aureus (CA-MRSA) strains appear to have rapidly disseminated among population
in the community without established risk factors for MRSA worldwide.
Panton-Valentine leukocidin (PVL) is a cytolytic toxin, encoded by the
lukF-PV and lukF-PV genes. PVL may be the key toxin responsible for enhanced
virulence of CA-MRSA. The aim of this study was to detect the genes encoding
PVL in CA-MRSA isolates from healthy people from the District of Pomoravlje.
Methods. We took throat and nose swabs from healthy, employed persons with
mandatory sanitary examinations and analyzed the presence of MRSA, between
January 2011 and December 2012 in the District of Pomoravlje. Susceptibility
of isolated strains to cefoxitin was investigated by using disc diffusion
according to the recommendation of CLSI (Clinical Laboratory Standard
Institute), and by E test. The presence of penicillin-binding protein 2a
(PBP2a) in Staphylococci was detected using latex agglutination Slidex ®MRSA
Detection test. The gold standard, polymerase chain reaction (PCR) assay, was
used for detection of mecA gene and PVL gene, and typing of SCCmec region.
Results. Our investigation showed that staphylococcal carrier state was
present in 2.58% of 52,910 throat and nasal swabs, and in 50 of them (3.67%)
MRSA was isolated. Among these MRSA, 2 (4%) isolates were PVL-positive.
Conclusion. The prevalence of CAMRSA and the presence of PVL gene among
healthy, employed population in the District of Pomoravlje were low. The
values obtained in this study show that, our region is not significantly
different from the other parts of our country, nor from the other European
countries. |
| first_indexed | 2024-04-13T01:35:03Z |
| format | Article |
| id | doaj.art-332b77d1ec774dc090f2e5432e97c6ca |
| institution | Directory Open Access Journal |
| issn | 0042-8450 2406-0720 |
| language | English |
| last_indexed | 2024-04-13T01:35:03Z |
| publishDate | 2016-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-332b77d1ec774dc090f2e5432e97c6ca2022-12-22T03:08:22ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502406-07202016-01-0173325626010.2298/VSP140715003P0042-84501600003PPrevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of PomoravljePetrović-Jeremić Ljiljana0Kuljić-Kapulica Nada1Ristanović Elizabeta2Jošić Dragana3Lepšanović Zorica4Center for Clinical Microbiology, Public Health Institution, ĆuprijaMilitary Medical Academy, Institute for Microbiology, Belgrade + University of Defence, Faculty of Medicine of the Military Medical Academy, BelgradeMilitary Medical Academy, Institute for Microbiology, Belgrade + University of Defence, Faculty of Medicine of the Military Medical Academy, BelgradeInstitute of Soil Science, BelgradeUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade + Military Medical Academy, Institute for Epidemiology, BelgradeBackground/Aim. Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains appear to have rapidly disseminated among population in the community without established risk factors for MRSA worldwide. Panton-Valentine leukocidin (PVL) is a cytolytic toxin, encoded by the lukF-PV and lukF-PV genes. PVL may be the key toxin responsible for enhanced virulence of CA-MRSA. The aim of this study was to detect the genes encoding PVL in CA-MRSA isolates from healthy people from the District of Pomoravlje. Methods. We took throat and nose swabs from healthy, employed persons with mandatory sanitary examinations and analyzed the presence of MRSA, between January 2011 and December 2012 in the District of Pomoravlje. Susceptibility of isolated strains to cefoxitin was investigated by using disc diffusion according to the recommendation of CLSI (Clinical Laboratory Standard Institute), and by E test. The presence of penicillin-binding protein 2a (PBP2a) in Staphylococci was detected using latex agglutination Slidex ®MRSA Detection test. The gold standard, polymerase chain reaction (PCR) assay, was used for detection of mecA gene and PVL gene, and typing of SCCmec region. Results. Our investigation showed that staphylococcal carrier state was present in 2.58% of 52,910 throat and nasal swabs, and in 50 of them (3.67%) MRSA was isolated. Among these MRSA, 2 (4%) isolates were PVL-positive. Conclusion. The prevalence of CAMRSA and the presence of PVL gene among healthy, employed population in the District of Pomoravlje were low. The values obtained in this study show that, our region is not significantly different from the other parts of our country, nor from the other European countries.http://www.doiserbia.nb.rs/img/doi/0042-8450/2016/0042-84501600003P.pdfmethicillin resistancestaphylococcus aureuscommunity acquired infectionspolymerase chain reactionpantonvalentine leukocidinSerbia |
| spellingShingle | Petrović-Jeremić Ljiljana Kuljić-Kapulica Nada Ristanović Elizabeta Jošić Dragana Lepšanović Zorica Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje Vojnosanitetski Pregled methicillin resistance staphylococcus aureus community acquired infections polymerase chain reaction pantonvalentine leukocidin Serbia |
| title | Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje |
| title_full | Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje |
| title_fullStr | Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje |
| title_full_unstemmed | Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje |
| title_short | Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje |
| title_sort | prevalence of panton valentine leukocidin genes in community associated methicillin resistant staphylococcus aureus in the district of pomoravlje |
| topic | methicillin resistance staphylococcus aureus community acquired infections polymerase chain reaction pantonvalentine leukocidin Serbia |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2016/0042-84501600003P.pdf |
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