CRF-like diuretic hormone negatively affects both feeding and reproduction in the desert locust, Schistocerca gregaria.
Diuretic hormones (DH) related to the vertebrate Corticotropin Releasing Factor (CRF) have been identified in diverse insect species. In the migratory locust, Locusta migratoria, the CRF-like DH (CRF/DH) is localized in the same neurosecretory cells as the Ovary Maturating Parsin (OMP), a neurohormo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3282710?pdf=render |
| _version_ | 1818858990156644352 |
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| author | Pieter Van Wielendaele Senne Dillen Elisabeth Marchal Liesbeth Badisco Jozef Vanden Broeck |
| author_facet | Pieter Van Wielendaele Senne Dillen Elisabeth Marchal Liesbeth Badisco Jozef Vanden Broeck |
| author_sort | Pieter Van Wielendaele |
| collection | DOAJ |
| description | Diuretic hormones (DH) related to the vertebrate Corticotropin Releasing Factor (CRF) have been identified in diverse insect species. In the migratory locust, Locusta migratoria, the CRF-like DH (CRF/DH) is localized in the same neurosecretory cells as the Ovary Maturating Parsin (OMP), a neurohormone that stimulates oocyte growth, vitellogenesis and hemolymph ecdysteroid levels in adult female locusts. In this study, we investigated whether CRF-like DH can influence feeding and reproduction in the desert locust, Schistocerca gregaria. We identified two highly similar S. gregaria CRF-like DH precursor cDNAs, each of which also encodes an OMP isoform. Alignment with other insect CRF-like DH precursors shows relatively high conservation of the CRF/DH sequence while the precursor region corresponding to OMP is not well conserved. Quantitative real-time RT-PCR revealed that the precursor transcripts mainly occur in the central nervous system and their highest expression level was observed in the brain. Injection of locust CRF/DH caused a significantly reduced food intake, while RNAi knockdown stimulated food intake. Therefore, our data indicate that CRF-like DH induces satiety. Furthermore, injection of CRF/DH in adult females retarded oocyte growth and caused lower ecdysteroid titers in hemolymph and ovaries, while RNAi knockdown resulted in opposite effects. The observed effects of CRF/DH may be part of a wider repertoire of neurohormonal activities, constituting an integrating control system that affects food intake and excretion, as well as anabolic processes like oocyte growth and ecdysteroidogenesis, following a meal. Our discussion about the functional relationship between CRF/DH and OMP led to the hypothesis that OMP may possibly act as a monitoring peptide that can elicit negative feedback effects. |
| first_indexed | 2024-12-19T09:05:04Z |
| format | Article |
| id | doaj.art-332c9ddbec8a4eabaae0a50b1ab1f587 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T09:05:04Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-332c9ddbec8a4eabaae0a50b1ab1f5872022-12-21T20:28:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3142510.1371/journal.pone.0031425CRF-like diuretic hormone negatively affects both feeding and reproduction in the desert locust, Schistocerca gregaria.Pieter Van WielendaeleSenne DillenElisabeth MarchalLiesbeth BadiscoJozef Vanden BroeckDiuretic hormones (DH) related to the vertebrate Corticotropin Releasing Factor (CRF) have been identified in diverse insect species. In the migratory locust, Locusta migratoria, the CRF-like DH (CRF/DH) is localized in the same neurosecretory cells as the Ovary Maturating Parsin (OMP), a neurohormone that stimulates oocyte growth, vitellogenesis and hemolymph ecdysteroid levels in adult female locusts. In this study, we investigated whether CRF-like DH can influence feeding and reproduction in the desert locust, Schistocerca gregaria. We identified two highly similar S. gregaria CRF-like DH precursor cDNAs, each of which also encodes an OMP isoform. Alignment with other insect CRF-like DH precursors shows relatively high conservation of the CRF/DH sequence while the precursor region corresponding to OMP is not well conserved. Quantitative real-time RT-PCR revealed that the precursor transcripts mainly occur in the central nervous system and their highest expression level was observed in the brain. Injection of locust CRF/DH caused a significantly reduced food intake, while RNAi knockdown stimulated food intake. Therefore, our data indicate that CRF-like DH induces satiety. Furthermore, injection of CRF/DH in adult females retarded oocyte growth and caused lower ecdysteroid titers in hemolymph and ovaries, while RNAi knockdown resulted in opposite effects. The observed effects of CRF/DH may be part of a wider repertoire of neurohormonal activities, constituting an integrating control system that affects food intake and excretion, as well as anabolic processes like oocyte growth and ecdysteroidogenesis, following a meal. Our discussion about the functional relationship between CRF/DH and OMP led to the hypothesis that OMP may possibly act as a monitoring peptide that can elicit negative feedback effects.http://europepmc.org/articles/PMC3282710?pdf=render |
| spellingShingle | Pieter Van Wielendaele Senne Dillen Elisabeth Marchal Liesbeth Badisco Jozef Vanden Broeck CRF-like diuretic hormone negatively affects both feeding and reproduction in the desert locust, Schistocerca gregaria. PLoS ONE |
| title | CRF-like diuretic hormone negatively affects both feeding and reproduction in the desert locust, Schistocerca gregaria. |
| title_full | CRF-like diuretic hormone negatively affects both feeding and reproduction in the desert locust, Schistocerca gregaria. |
| title_fullStr | CRF-like diuretic hormone negatively affects both feeding and reproduction in the desert locust, Schistocerca gregaria. |
| title_full_unstemmed | CRF-like diuretic hormone negatively affects both feeding and reproduction in the desert locust, Schistocerca gregaria. |
| title_short | CRF-like diuretic hormone negatively affects both feeding and reproduction in the desert locust, Schistocerca gregaria. |
| title_sort | crf like diuretic hormone negatively affects both feeding and reproduction in the desert locust schistocerca gregaria |
| url | http://europepmc.org/articles/PMC3282710?pdf=render |
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