Changes in expression of alpha-1, 2 and β-2 adenoidal receptor gene in cumulus cells of infertile women with polycystic ovary syndrome (IVF candidate) and effect of clonidine on it
Introduction: Ovarian function should control follicle development, steroidogenesis, and ovulation processes by ovarian external and internal hormonal signals. Sympathetic system Alpha and beta receptors play a role in regulation of theca and granulosa cells activity for folliculogenesis and steroid...
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| Format: | Article |
| Language: | fas |
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Mashhad University of Medical Sciences
2019-07-01
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| Series: | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
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| Online Access: | https://ijogi.mums.ac.ir/article_13576_f5fde489ccdda068c2b77180f0eb8428.pdf |
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| author | Farideh Zafari Zangeneh Elaheh Aboutorabi Masoumeh Dehghan Sara Zabihzadeh |
| author_facet | Farideh Zafari Zangeneh Elaheh Aboutorabi Masoumeh Dehghan Sara Zabihzadeh |
| author_sort | Farideh Zafari Zangeneh |
| collection | DOAJ |
| description | Introduction: Ovarian function should control follicle development, steroidogenesis, and ovulation processes by ovarian external and internal hormonal signals. Sympathetic system Alpha and beta receptors play a role in regulation of theca and granulosa cells activity for folliculogenesis and steroidogenesis. This study was performed with aim to investigate the changes in expression of alpha 1, 2 and beta 2 adrenergic receptors gene of ovary cumulus in infertile women with polycystic ovarian syndrome (IVF candidate) and effect of clonidine on it. Methods: This case-control study was conducted in 2017 on two groups of control (healthy women donating oocytes) and case (patients with polycystic ovaries). Ovulation induction drugs were prescribed in two groups for ovulation growth. After oocyte puncture, the follicular fluid was collected with cumulus-oocyte collection. The cumulus cells were isolated by enzyme and were cultured. RNA was then extracted from cumulus cells and its concentration was read by Nano-drop device. cDNA was synthesized and primer was designed for ADRB2 and ADRα1.2 gene expression and the gene expression was assessed by Real-time PCR technique. Finally, the effect of clonidine on protein level of ADRβ2 and ADRα1.2 genes in cumulus cells was investigated by immunocytochemistry technique (western blot) in two groups. Results: The results of this study confirmed: 1) increasing of gene expression level in women with PCOS compared with control group and 2) efficacy of clonidine on gene expression. The severity of the drug efficacy on receptors gene expression was ADRα1 <ADRβ2 <ADRα2, respectively. Conclusion: The results of gene expression and immunocytochemistry showed that clonidine could significantly reduce gene expression of three adrenergic receptors, especially α2 in women with PCO, and therefore clonidine pharmacotherapy could be suggested. |
| first_indexed | 2024-04-13T15:34:06Z |
| format | Article |
| id | doaj.art-332e515272e8470f9ea57a379c709b9c |
| institution | Directory Open Access Journal |
| issn | 1680-2993 2008-2363 |
| language | fas |
| last_indexed | 2024-04-13T15:34:06Z |
| publishDate | 2019-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
| spelling | doaj.art-332e515272e8470f9ea57a379c709b9c2022-12-22T02:41:18ZfasMashhad University of Medical SciencesMajallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān1680-29932008-23632019-07-0122511210.22038/ijogi.2019.1357613576Changes in expression of alpha-1, 2 and β-2 adenoidal receptor gene in cumulus cells of infertile women with polycystic ovary syndrome (IVF candidate) and effect of clonidine on itFarideh Zafari Zangeneh0Elaheh Aboutorabi1Masoumeh Dehghan2Sara Zabihzadeh3Associate Professor, Vali-e-Asr Reproductive Health Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.PhD student in Genetics, Islamic Azad University Sciences and Research Center, Tehran, Iran.PhD in Reproduction, Vali-e-Asr Reproductive Health Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.M.Sc. student in Biochemistry, Islamic Azad University, Tehran, Iran.Introduction: Ovarian function should control follicle development, steroidogenesis, and ovulation processes by ovarian external and internal hormonal signals. Sympathetic system Alpha and beta receptors play a role in regulation of theca and granulosa cells activity for folliculogenesis and steroidogenesis. This study was performed with aim to investigate the changes in expression of alpha 1, 2 and beta 2 adrenergic receptors gene of ovary cumulus in infertile women with polycystic ovarian syndrome (IVF candidate) and effect of clonidine on it. Methods: This case-control study was conducted in 2017 on two groups of control (healthy women donating oocytes) and case (patients with polycystic ovaries). Ovulation induction drugs were prescribed in two groups for ovulation growth. After oocyte puncture, the follicular fluid was collected with cumulus-oocyte collection. The cumulus cells were isolated by enzyme and were cultured. RNA was then extracted from cumulus cells and its concentration was read by Nano-drop device. cDNA was synthesized and primer was designed for ADRB2 and ADRα1.2 gene expression and the gene expression was assessed by Real-time PCR technique. Finally, the effect of clonidine on protein level of ADRβ2 and ADRα1.2 genes in cumulus cells was investigated by immunocytochemistry technique (western blot) in two groups. Results: The results of this study confirmed: 1) increasing of gene expression level in women with PCOS compared with control group and 2) efficacy of clonidine on gene expression. The severity of the drug efficacy on receptors gene expression was ADRα1 <ADRβ2 <ADRα2, respectively. Conclusion: The results of gene expression and immunocytochemistry showed that clonidine could significantly reduce gene expression of three adrenergic receptors, especially α2 in women with PCO, and therefore clonidine pharmacotherapy could be suggested.https://ijogi.mums.ac.ir/article_13576_f5fde489ccdda068c2b77180f0eb8428.pdfclonidinegene expressionimmunocytochemistrypolycystic ovary syndromesympathetic nervous system |
| spellingShingle | Farideh Zafari Zangeneh Elaheh Aboutorabi Masoumeh Dehghan Sara Zabihzadeh Changes in expression of alpha-1, 2 and β-2 adenoidal receptor gene in cumulus cells of infertile women with polycystic ovary syndrome (IVF candidate) and effect of clonidine on it Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān clonidine gene expression immunocytochemistry polycystic ovary syndrome sympathetic nervous system |
| title | Changes in expression of alpha-1, 2 and β-2 adenoidal receptor gene in cumulus cells of infertile women with polycystic ovary syndrome (IVF candidate) and effect of clonidine on it |
| title_full | Changes in expression of alpha-1, 2 and β-2 adenoidal receptor gene in cumulus cells of infertile women with polycystic ovary syndrome (IVF candidate) and effect of clonidine on it |
| title_fullStr | Changes in expression of alpha-1, 2 and β-2 adenoidal receptor gene in cumulus cells of infertile women with polycystic ovary syndrome (IVF candidate) and effect of clonidine on it |
| title_full_unstemmed | Changes in expression of alpha-1, 2 and β-2 adenoidal receptor gene in cumulus cells of infertile women with polycystic ovary syndrome (IVF candidate) and effect of clonidine on it |
| title_short | Changes in expression of alpha-1, 2 and β-2 adenoidal receptor gene in cumulus cells of infertile women with polycystic ovary syndrome (IVF candidate) and effect of clonidine on it |
| title_sort | changes in expression of alpha 1 2 and β 2 adenoidal receptor gene in cumulus cells of infertile women with polycystic ovary syndrome ivf candidate and effect of clonidine on it |
| topic | clonidine gene expression immunocytochemistry polycystic ovary syndrome sympathetic nervous system |
| url | https://ijogi.mums.ac.ir/article_13576_f5fde489ccdda068c2b77180f0eb8428.pdf |
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