Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic Acid
Background: Tetracycline is a broad-spectrum antibiotic that is used to treat diseases caused by different types of bacteria. Objective:To estimate tetracycline hydrochloride in pharmaceuticals through coupling with a new reagent, sulfanilic acid. Patients and Methods: The proposed method for...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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College of medicine/ University of Diyala
2018-12-01
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| Series: | Diyala Journal of Medicine |
| Subjects: | |
| Online Access: | https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/172 |
| Summary: | Background: Tetracycline is a broad-spectrum antibiotic that is used to treat diseases caused by different types of bacteria.
Objective:To estimate tetracycline hydrochloride in pharmaceuticals through coupling with a new reagent, sulfanilic acid.
Patients and Methods: The proposed method for determination of tetracycline hydrochloride was applied to four different types of pharmaceutical drug companies and the results were compared with a standard method (HPLC method) to determine their compatibility and the results were satisfactory.
Results: The method is based on azo coupling reaction between sulfanilic acid and tetracycline in alkaline medium. The colored compound produced by the reaction has a higher absorbance at a wavelength of 403nm. The Beer ,s law was applied in the concentration range (8.0 - 95.0μg / ml) and the detection limit, molar absorptivity and correlation coefficient were 0.2μg / ml, 6.536x103L / mol.cm and 0.9997 respectively. The method was successfully applied to the evaluation of tetracycline hydrochloride in pharmaceuticals.
Conclusion:The proposed method for determination of tetracycline hydrochloride is a simple and sensitive method, where the tetracycline hydrochloride can be estimated at low concentration and accurately. This method needs a simple device and the method can be used in quality control centers. The recommended method has been successfully applied to estimate tetracycline hydrochloride in pharmaceuticals.
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| ISSN: | 2219-9764 2617-8982 |