Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic Acid
Background: Tetracycline is a broad-spectrum antibiotic that is used to treat diseases caused by different types of bacteria. Objective:To estimate tetracycline hydrochloride in pharmaceuticals through coupling with a new reagent, sulfanilic acid. Patients and Methods: The proposed method for...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
College of medicine/ University of Diyala
2018-12-01
|
| Series: | Diyala Journal of Medicine |
| Subjects: | |
| Online Access: | https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/172 |
| _version_ | 1797586297816088576 |
|---|---|
| author | Rasul J Ali Hawar J Sadiq Hawezy Mohammad S Abdullah |
| author_facet | Rasul J Ali Hawar J Sadiq Hawezy Mohammad S Abdullah |
| author_sort | Rasul J Ali |
| collection | DOAJ |
| description |
Background: Tetracycline is a broad-spectrum antibiotic that is used to treat diseases caused by different types of bacteria.
Objective:To estimate tetracycline hydrochloride in pharmaceuticals through coupling with a new reagent, sulfanilic acid.
Patients and Methods: The proposed method for determination of tetracycline hydrochloride was applied to four different types of pharmaceutical drug companies and the results were compared with a standard method (HPLC method) to determine their compatibility and the results were satisfactory.
Results: The method is based on azo coupling reaction between sulfanilic acid and tetracycline in alkaline medium. The colored compound produced by the reaction has a higher absorbance at a wavelength of 403nm. The Beer ,s law was applied in the concentration range (8.0 - 95.0μg / ml) and the detection limit, molar absorptivity and correlation coefficient were 0.2μg / ml, 6.536x103L / mol.cm and 0.9997 respectively. The method was successfully applied to the evaluation of tetracycline hydrochloride in pharmaceuticals.
Conclusion:The proposed method for determination of tetracycline hydrochloride is a simple and sensitive method, where the tetracycline hydrochloride can be estimated at low concentration and accurately. This method needs a simple device and the method can be used in quality control centers. The recommended method has been successfully applied to estimate tetracycline hydrochloride in pharmaceuticals.
|
| first_indexed | 2024-03-11T00:21:20Z |
| format | Article |
| id | doaj.art-333392569c2a440a8a0fe65555289262 |
| institution | Directory Open Access Journal |
| issn | 2219-9764 2617-8982 |
| language | English |
| last_indexed | 2024-03-11T00:21:20Z |
| publishDate | 2018-12-01 |
| publisher | College of medicine/ University of Diyala |
| record_format | Article |
| series | Diyala Journal of Medicine |
| spelling | doaj.art-333392569c2a440a8a0fe655552892622023-11-18T23:17:57ZengCollege of medicine/ University of DiyalaDiyala Journal of Medicine2219-97642617-89822018-12-01152Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic AcidRasul J AliHawar J Sadiq HawezyMohammad S Abdullah Background: Tetracycline is a broad-spectrum antibiotic that is used to treat diseases caused by different types of bacteria. Objective:To estimate tetracycline hydrochloride in pharmaceuticals through coupling with a new reagent, sulfanilic acid. Patients and Methods: The proposed method for determination of tetracycline hydrochloride was applied to four different types of pharmaceutical drug companies and the results were compared with a standard method (HPLC method) to determine their compatibility and the results were satisfactory. Results: The method is based on azo coupling reaction between sulfanilic acid and tetracycline in alkaline medium. The colored compound produced by the reaction has a higher absorbance at a wavelength of 403nm. The Beer ,s law was applied in the concentration range (8.0 - 95.0μg / ml) and the detection limit, molar absorptivity and correlation coefficient were 0.2μg / ml, 6.536x103L / mol.cm and 0.9997 respectively. The method was successfully applied to the evaluation of tetracycline hydrochloride in pharmaceuticals. Conclusion:The proposed method for determination of tetracycline hydrochloride is a simple and sensitive method, where the tetracycline hydrochloride can be estimated at low concentration and accurately. This method needs a simple device and the method can be used in quality control centers. The recommended method has been successfully applied to estimate tetracycline hydrochloride in pharmaceuticals. https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/172Spectrophotometry, Tetracycline, Azo-coupling |
| spellingShingle | Rasul J Ali Hawar J Sadiq Hawezy Mohammad S Abdullah Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic Acid Diyala Journal of Medicine Spectrophotometry, Tetracycline, Azo-coupling |
| title | Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic Acid |
| title_full | Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic Acid |
| title_fullStr | Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic Acid |
| title_full_unstemmed | Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic Acid |
| title_short | Spectrophotometric Determination of Tetracycline Hydrochloride Through Coupling with Sulphanilic Acid |
| title_sort | spectrophotometric determination of tetracycline hydrochloride through coupling with sulphanilic acid |
| topic | Spectrophotometry, Tetracycline, Azo-coupling |
| url | https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/172 |
| work_keys_str_mv | AT rasuljali spectrophotometricdeterminationoftetracyclinehydrochloridethroughcouplingwithsulphanilicacid AT hawarjsadiqhawezy spectrophotometricdeterminationoftetracyclinehydrochloridethroughcouplingwithsulphanilicacid AT mohammadsabdullah spectrophotometricdeterminationoftetracyclinehydrochloridethroughcouplingwithsulphanilicacid |