EFFECT OF REDUCING SPERM NUMBERS PER INSEMINATION DOSE ON FERTILITY OF CRYOPRESERVED BUFFALO BULL SEMEN
The objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2006-01-01
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| Series: | Pakistan Veterinary Journal |
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| Online Access: | http://pvj.com.pk/pdf-files/26_1/17-19.pdf |
| _version_ | 1811284352669057024 |
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| author | S. M. H. ANDRABI, M. SIDDIQUE1, N. ULLAH AND L. A. KHAN |
| author_facet | S. M. H. ANDRABI, M. SIDDIQUE1, N. ULLAH AND L. A. KHAN |
| author_sort | S. M. H. ANDRABI, M. SIDDIQUE1, N. ULLAH AND L. A. KHAN |
| collection | DOAJ |
| description | The objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches. The ejaculates were split-sampled and diluted at 37°C with tris-citric acid extender having 15x106 or 30x106 motile spermatozoa/0.5 ml. After dilution, the semen was cooled to 4C, equilibrated for 4 hours, packaged in 0.5 ml straws and frozen in programmable cell freezer. Fertility test based on 75-days first service pregnancy rate was determined under field conditions. A total of 500 buffaloes were inseminated with frozen semen and out of these 431 could be followed, 209 for semen straws packaged with 15x106 spermatozoa/straw and 222 for doses filled with 30x106 spermatozoa/straw. The inseminations were performed in two batches and each batch was spread over a period of three months. The fertility rate for sperm concentration of 15x106 spermatozoa/0.5 ml vs. 30x106 spermatozoa/0.5 ml (49.28 vs. 56.75%) was similar (P>0.05). The fertility rates were also similar (P>0.05) in the first and second batch of inseminations performed with 15x106 or 30x106 spermatozoa/0.5 ml straw of cryopreserved semen. In conclusion, reduction of sperm number from 30x106 to 15x106 spermatozoa/0.5 ml dose of insemination did not affect fertility of cryopreserved buffalo bull semen. |
| first_indexed | 2024-04-13T02:27:26Z |
| format | Article |
| id | doaj.art-3341e6648b7e4553ba8b6757621c74cc |
| institution | Directory Open Access Journal |
| issn | 0253-8318 |
| language | English |
| last_indexed | 2024-04-13T02:27:26Z |
| publishDate | 2006-01-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-3341e6648b7e4553ba8b6757621c74cc2022-12-22T03:06:43ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182006-01-012611719EFFECT OF REDUCING SPERM NUMBERS PER INSEMINATION DOSE ON FERTILITY OF CRYOPRESERVED BUFFALO BULL SEMENS. M. H. ANDRABI, M. SIDDIQUE1, N. ULLAH AND L. A. KHANThe objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches. The ejaculates were split-sampled and diluted at 37°C with tris-citric acid extender having 15x106 or 30x106 motile spermatozoa/0.5 ml. After dilution, the semen was cooled to 4C, equilibrated for 4 hours, packaged in 0.5 ml straws and frozen in programmable cell freezer. Fertility test based on 75-days first service pregnancy rate was determined under field conditions. A total of 500 buffaloes were inseminated with frozen semen and out of these 431 could be followed, 209 for semen straws packaged with 15x106 spermatozoa/straw and 222 for doses filled with 30x106 spermatozoa/straw. The inseminations were performed in two batches and each batch was spread over a period of three months. The fertility rate for sperm concentration of 15x106 spermatozoa/0.5 ml vs. 30x106 spermatozoa/0.5 ml (49.28 vs. 56.75%) was similar (P>0.05). The fertility rates were also similar (P>0.05) in the first and second batch of inseminations performed with 15x106 or 30x106 spermatozoa/0.5 ml straw of cryopreserved semen. In conclusion, reduction of sperm number from 30x106 to 15x106 spermatozoa/0.5 ml dose of insemination did not affect fertility of cryopreserved buffalo bull semen.http://pvj.com.pk/pdf-files/26_1/17-19.pdfSperm numberfrozen semenfertilityAIbuffalo |
| spellingShingle | S. M. H. ANDRABI, M. SIDDIQUE1, N. ULLAH AND L. A. KHAN EFFECT OF REDUCING SPERM NUMBERS PER INSEMINATION DOSE ON FERTILITY OF CRYOPRESERVED BUFFALO BULL SEMEN Pakistan Veterinary Journal Sperm number frozen semen fertility AI buffalo |
| title | EFFECT OF REDUCING SPERM NUMBERS PER INSEMINATION DOSE ON FERTILITY OF CRYOPRESERVED BUFFALO BULL SEMEN |
| title_full | EFFECT OF REDUCING SPERM NUMBERS PER INSEMINATION DOSE ON FERTILITY OF CRYOPRESERVED BUFFALO BULL SEMEN |
| title_fullStr | EFFECT OF REDUCING SPERM NUMBERS PER INSEMINATION DOSE ON FERTILITY OF CRYOPRESERVED BUFFALO BULL SEMEN |
| title_full_unstemmed | EFFECT OF REDUCING SPERM NUMBERS PER INSEMINATION DOSE ON FERTILITY OF CRYOPRESERVED BUFFALO BULL SEMEN |
| title_short | EFFECT OF REDUCING SPERM NUMBERS PER INSEMINATION DOSE ON FERTILITY OF CRYOPRESERVED BUFFALO BULL SEMEN |
| title_sort | effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen |
| topic | Sperm number frozen semen fertility AI buffalo |
| url | http://pvj.com.pk/pdf-files/26_1/17-19.pdf |
| work_keys_str_mv | AT smhandrabimsiddique1nullahandlakhan effectofreducingspermnumbersperinseminationdoseonfertilityofcryopreservedbuffalobullsemen |