Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11
Abstract Background Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite lo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | BMC Biotechnology |
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| Online Access: | http://link.springer.com/article/10.1186/s12896-018-0478-2 |
| _version_ | 1818294523285995520 |
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| author | Bao Tang Cheng Sun Yancun Zhao Huiyong Xu Gaoge Xu Fengquan Liu |
| author_facet | Bao Tang Cheng Sun Yancun Zhao Huiyong Xu Gaoge Xu Fengquan Liu |
| author_sort | Bao Tang |
| collection | DOAJ |
| description | Abstract Background Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has reported on the fermentation process involved, representing huge obstacles for large-scale industrial production. Results Medium capacity, culture temperature, and fermentation time were identified as the most significant factors affecting the production of HSAF and employed for further optimization through statistical methods. Based on the analysis of kinetic parameters at different temperatures, a novel two-stage temperature control strategy was developed to improve HSAF production, in which the temperature was increased to 32 °C during the first 12 h and then switched to 26 °C until the end of fermentation. Using this strategy, the maximum HSAF production reached 440.26 ± 16.14 mg L− 1, increased by 9.93% than that of the best results from single-temperature fermentation. Moreover, the fermentation time was shortened from 58 h to 54 h, resulting in the enhancement of HSAF productivity (17.95%) and yield (9.93%). Conclusions This study provides a simple and efficient method for producing HSAF that could be feasibly applied to the industrial-scale production of HSAF. |
| first_indexed | 2024-12-13T03:33:06Z |
| format | Article |
| id | doaj.art-33425a59bb2946889375cc2ee5c5f827 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-13T03:33:06Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-33425a59bb2946889375cc2ee5c5f8272022-12-22T00:01:07ZengBMCBMC Biotechnology1472-67502018-10-011811910.1186/s12896-018-0478-2Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11Bao Tang0Cheng Sun1Yancun Zhao2Huiyong Xu3Gaoge Xu4Fengquan Liu5Institute of Plant Protection, Jiangsu Academy of Agricultural SciencesSchool of Resources and Environmental engineering, Yangzhou Polytechnic CollegeInstitute of Plant Protection, Jiangsu Academy of Agricultural SciencesInstitute of Plant Protection, Jiangsu Academy of Agricultural SciencesInstitute of Plant Protection, Jiangsu Academy of Agricultural SciencesInstitute of Plant Protection, Jiangsu Academy of Agricultural SciencesAbstract Background Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has reported on the fermentation process involved, representing huge obstacles for large-scale industrial production. Results Medium capacity, culture temperature, and fermentation time were identified as the most significant factors affecting the production of HSAF and employed for further optimization through statistical methods. Based on the analysis of kinetic parameters at different temperatures, a novel two-stage temperature control strategy was developed to improve HSAF production, in which the temperature was increased to 32 °C during the first 12 h and then switched to 26 °C until the end of fermentation. Using this strategy, the maximum HSAF production reached 440.26 ± 16.14 mg L− 1, increased by 9.93% than that of the best results from single-temperature fermentation. Moreover, the fermentation time was shortened from 58 h to 54 h, resulting in the enhancement of HSAF productivity (17.95%) and yield (9.93%). Conclusions This study provides a simple and efficient method for producing HSAF that could be feasibly applied to the industrial-scale production of HSAF.http://link.springer.com/article/10.1186/s12896-018-0478-2Heat-stable antifungal factorLysobacter enzymogenes OH11BiopesticidesTwo-stage temperature control strategy |
| spellingShingle | Bao Tang Cheng Sun Yancun Zhao Huiyong Xu Gaoge Xu Fengquan Liu Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11 BMC Biotechnology Heat-stable antifungal factor Lysobacter enzymogenes OH11 Biopesticides Two-stage temperature control strategy |
| title | Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11 |
| title_full | Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11 |
| title_fullStr | Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11 |
| title_full_unstemmed | Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11 |
| title_short | Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11 |
| title_sort | efficient production of heat stable antifungal factor through integrating statistical optimization with a two stage temperature control strategy in lysobacter enzymogenes oh11 |
| topic | Heat-stable antifungal factor Lysobacter enzymogenes OH11 Biopesticides Two-stage temperature control strategy |
| url | http://link.springer.com/article/10.1186/s12896-018-0478-2 |
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