Cancers Cells in Traps? The Pathways of NETs Formation in Response to OSCC in Humans—A Pilot Study
The aim of the experiment was to evaluate the process of neutrophil extracellular traps (NETs) formation in patients with oral squamous cell carcinoma (OSCC) in response to direct or indirect contact with SCC cells in comparison to results obtained in the cells of healthy subjects. To fulfill study...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-10-01
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| Series: | Cancer Control |
| Online Access: | https://doi.org/10.1177/1073274820960473 |
| _version_ | 1827893006400749568 |
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| author | Marzena Garley Ewa Jabłońska Wojciech Miltyk Kamil Grubczak Arkadiusz Surażyński Wioletta Ratajczak-Wrona Małgorzata Grudzińska Kinga H. Nowacka Marcin Moniuszko Jerzy A. Pałka Jan Borys Dorota Dziemiańczyk-Pakieła |
| author_facet | Marzena Garley Ewa Jabłońska Wojciech Miltyk Kamil Grubczak Arkadiusz Surażyński Wioletta Ratajczak-Wrona Małgorzata Grudzińska Kinga H. Nowacka Marcin Moniuszko Jerzy A. Pałka Jan Borys Dorota Dziemiańczyk-Pakieła |
| author_sort | Marzena Garley |
| collection | DOAJ |
| description | The aim of the experiment was to evaluate the process of neutrophil extracellular traps (NETs) formation in patients with oral squamous cell carcinoma (OSCC) in response to direct or indirect contact with SCC cells in comparison to results obtained in the cells of healthy subjects. To fulfill study objectives CAL 27 cell line and blood were obtained from cancer patients and control subjects. Parameters related to NETs formation were analyzed utilizing flow cytometry, fluorescence microscopy, and ELISA-type tests. The expression of selected phosphorylated proteins of the PI3K/Akt/PBK pathway in neutrophils was evaluated using the Western blot method. An increase in NETs formation was observed in a coculture of neutrophils with SCC cells, with the largest amount of NETs formed after stimulation with a supernatant obtained from the SCC culture. The enhanced process of NETs formation was accompanied by changes in the expression of proteins from the PI3K/Akt/PBK pathway. The obtained results prove the existence of interactions between neutrophils and cancer cells resulting in NETosis with the participation of the PI3K/Akt/PBK pathway in patients with OSCC. |
| first_indexed | 2024-03-12T21:48:01Z |
| format | Article |
| id | doaj.art-3347eff948174c58ac9259ed637f6151 |
| institution | Directory Open Access Journal |
| issn | 1073-2748 |
| language | English |
| last_indexed | 2024-03-12T21:48:01Z |
| publishDate | 2020-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cancer Control |
| spelling | doaj.art-3347eff948174c58ac9259ed637f61512023-07-26T07:06:20ZengSAGE PublishingCancer Control1073-27482020-10-012710.1177/1073274820960473Cancers Cells in Traps? The Pathways of NETs Formation in Response to OSCC in Humans—A Pilot StudyMarzena Garley0Ewa Jabłońska1Wojciech Miltyk2Kamil Grubczak3Arkadiusz Surażyński4Wioletta Ratajczak-Wrona5Małgorzata Grudzińska6Kinga H. Nowacka7Marcin Moniuszko8Jerzy A. Pałka9Jan Borys10Dorota Dziemiańczyk-Pakieła11 , Poland , Poland Department of Pharmaceutical and Biopharmaceutical Analysis, The Centre for Innovative Research, , Poland Department of Regenerative Medicine and Immune Regulation, , Poland Department of Medicinal Chemistry, The Centre for Innovative Research, , Poland , Poland Department of Pathomorphology, , Poland , Poland Department of Regenerative Medicine and Immune Regulation, , Poland Department of Medicinal Chemistry, The Centre for Innovative Research, , Poland Department of Maxillofacial and Plastic Surgery, , Poland Otolaryngology and Maxillofacial Surgery Ward of the Provincial Integrated Hospital Jędrzej Śniadecki in Bialystok, PolandThe aim of the experiment was to evaluate the process of neutrophil extracellular traps (NETs) formation in patients with oral squamous cell carcinoma (OSCC) in response to direct or indirect contact with SCC cells in comparison to results obtained in the cells of healthy subjects. To fulfill study objectives CAL 27 cell line and blood were obtained from cancer patients and control subjects. Parameters related to NETs formation were analyzed utilizing flow cytometry, fluorescence microscopy, and ELISA-type tests. The expression of selected phosphorylated proteins of the PI3K/Akt/PBK pathway in neutrophils was evaluated using the Western blot method. An increase in NETs formation was observed in a coculture of neutrophils with SCC cells, with the largest amount of NETs formed after stimulation with a supernatant obtained from the SCC culture. The enhanced process of NETs formation was accompanied by changes in the expression of proteins from the PI3K/Akt/PBK pathway. The obtained results prove the existence of interactions between neutrophils and cancer cells resulting in NETosis with the participation of the PI3K/Akt/PBK pathway in patients with OSCC.https://doi.org/10.1177/1073274820960473 |
| spellingShingle | Marzena Garley Ewa Jabłońska Wojciech Miltyk Kamil Grubczak Arkadiusz Surażyński Wioletta Ratajczak-Wrona Małgorzata Grudzińska Kinga H. Nowacka Marcin Moniuszko Jerzy A. Pałka Jan Borys Dorota Dziemiańczyk-Pakieła Cancers Cells in Traps? The Pathways of NETs Formation in Response to OSCC in Humans—A Pilot Study Cancer Control |
| title | Cancers Cells in Traps? The Pathways of NETs Formation in Response to OSCC in Humans—A Pilot Study |
| title_full | Cancers Cells in Traps? The Pathways of NETs Formation in Response to OSCC in Humans—A Pilot Study |
| title_fullStr | Cancers Cells in Traps? The Pathways of NETs Formation in Response to OSCC in Humans—A Pilot Study |
| title_full_unstemmed | Cancers Cells in Traps? The Pathways of NETs Formation in Response to OSCC in Humans—A Pilot Study |
| title_short | Cancers Cells in Traps? The Pathways of NETs Formation in Response to OSCC in Humans—A Pilot Study |
| title_sort | cancers cells in traps the pathways of nets formation in response to oscc in humans a pilot study |
| url | https://doi.org/10.1177/1073274820960473 |
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