Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke
The hippocampus is often injured in neonatal stroke. We have investigated the effect of erythropoietin (EPO) on oxygen-glucose deprived hippocampal slices and hypoxic progenitor cells. EPO improved survival of the organotypic hippocampal slices with significantly less cell death in the dentate gyrus...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010-05-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996110000318 |
| _version_ | 1818862563006349312 |
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| author | Damjan Osredkar Jeffrey W. Sall Philip E. Bickler Donna M. Ferriero |
| author_facet | Damjan Osredkar Jeffrey W. Sall Philip E. Bickler Donna M. Ferriero |
| author_sort | Damjan Osredkar |
| collection | DOAJ |
| description | The hippocampus is often injured in neonatal stroke. We have investigated the effect of erythropoietin (EPO) on oxygen-glucose deprived hippocampal slices and hypoxic progenitor cells. EPO improved survival of the organotypic hippocampal slices with significantly less cell death in the dentate gyrus and an increased number of proliferating cells 4–5 days after insult. Significantly fewer markers of neurogenesis were seen after the insult but when EPO was added to the culture medium, neurogenesis was sustained. When hippocampal progenitor cultures were stimulated into differentiation, more cells chose a neuronal cell fate when treated with EPO. These findings support the hypothesis that EPO not only prevents ischemia induced cell death but promotes neuronal cell fate commitment in in vitro models of neonatal stroke. |
| first_indexed | 2024-12-19T10:01:51Z |
| format | Article |
| id | doaj.art-334c5cbd730d44978ec8755f90f51021 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-19T10:01:51Z |
| publishDate | 2010-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-334c5cbd730d44978ec8755f90f510212022-12-21T20:26:36ZengElsevierNeurobiology of Disease1095-953X2010-05-01382259265Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal strokeDamjan Osredkar0Jeffrey W. Sall1Philip E. Bickler2Donna M. Ferriero3Department of Neurology, University of California Box 0663, 521 Parnassus Avenue C215, San Francisco, CA 94143-0663, USA; Department of Pediatric Neurology, University Children's Hospital, Ljubljana, SloveniaDepartment of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USADepartment of Anesthesia and Perioperative Care, University of California, San Francisco, CA, USADepartment of Neurology, University of California Box 0663, 521 Parnassus Avenue C215, San Francisco, CA 94143-0663, USA; Department of Pediatrics, University of California, San Francisco, CA, USA; Corresponding author. Department of Neurology, University of California Box 0663, 521 Parnassus Avenue C215, San Francisco, CA 94143-0663, USA. Fax: +1 415 502 5821.The hippocampus is often injured in neonatal stroke. We have investigated the effect of erythropoietin (EPO) on oxygen-glucose deprived hippocampal slices and hypoxic progenitor cells. EPO improved survival of the organotypic hippocampal slices with significantly less cell death in the dentate gyrus and an increased number of proliferating cells 4–5 days after insult. Significantly fewer markers of neurogenesis were seen after the insult but when EPO was added to the culture medium, neurogenesis was sustained. When hippocampal progenitor cultures were stimulated into differentiation, more cells chose a neuronal cell fate when treated with EPO. These findings support the hypothesis that EPO not only prevents ischemia induced cell death but promotes neuronal cell fate commitment in in vitro models of neonatal stroke.http://www.sciencedirect.com/science/article/pii/S0969996110000318NeurogenesisHypoxiaErythropoietinHippocampusDifferentiationNeonate |
| spellingShingle | Damjan Osredkar Jeffrey W. Sall Philip E. Bickler Donna M. Ferriero Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke Neurobiology of Disease Neurogenesis Hypoxia Erythropoietin Hippocampus Differentiation Neonate |
| title | Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke |
| title_full | Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke |
| title_fullStr | Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke |
| title_full_unstemmed | Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke |
| title_short | Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke |
| title_sort | erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke |
| topic | Neurogenesis Hypoxia Erythropoietin Hippocampus Differentiation Neonate |
| url | http://www.sciencedirect.com/science/article/pii/S0969996110000318 |
| work_keys_str_mv | AT damjanosredkar erythropoietinpromoteshippocampalneurogenesisininvitromodelsofneonatalstroke AT jeffreywsall erythropoietinpromoteshippocampalneurogenesisininvitromodelsofneonatalstroke AT philipebickler erythropoietinpromoteshippocampalneurogenesisininvitromodelsofneonatalstroke AT donnamferriero erythropoietinpromoteshippocampalneurogenesisininvitromodelsofneonatalstroke |