Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9
The Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is a alpha subunit of the PP1 complex, which known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Incre...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123000636 |
| _version_ | 1797816034496872448 |
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| author | Xiaoman Wang Jiaan Sun Chong Fu Chang Liu |
| author_facet | Xiaoman Wang Jiaan Sun Chong Fu Chang Liu |
| author_sort | Xiaoman Wang |
| collection | DOAJ |
| description | The Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is a alpha subunit of the PP1 complex, which known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Here, a PPP1CA knockout human embryonic stem cell line, WAe009-A-B, was generated using the CRISPR/Cas9 system to further study the function of PPP1CA. The cell line confirmed with pluripotency, normal karyotype and differentiation potential. |
| first_indexed | 2024-03-13T08:31:41Z |
| format | Article |
| id | doaj.art-334d079a8ad3479ca3f253ffb8debb23 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-13T08:31:41Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-334d079a8ad3479ca3f253ffb8debb232023-05-31T04:44:06ZengElsevierStem Cell Research1873-50612023-06-0169103077Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9Xiaoman Wang0Jiaan Sun1Chong Fu2Chang Liu3Department of Emergency, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, ChinaDepartment of Emergency, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, ChinaHenan Institute of Metrology, No. 10 Baifo Road, Guancheng District, Zhengzhou 450008, ChinaDepartment of Emergency, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, China; Corresponding author at: Department of Emergency, Zhengzhou Central Hospital Affiliated to Zhengzhou University, No.16 Tongbai North Road, Zhongyuan District, Zhengzhou 450007, China.The Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is a alpha subunit of the PP1 complex, which known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Here, a PPP1CA knockout human embryonic stem cell line, WAe009-A-B, was generated using the CRISPR/Cas9 system to further study the function of PPP1CA. The cell line confirmed with pluripotency, normal karyotype and differentiation potential.http://www.sciencedirect.com/science/article/pii/S1873506123000636 |
| spellingShingle | Xiaoman Wang Jiaan Sun Chong Fu Chang Liu Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9 Stem Cell Research |
| title | Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9 |
| title_full | Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9 |
| title_fullStr | Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9 |
| title_full_unstemmed | Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9 |
| title_short | Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9 |
| title_sort | generation of a ppp1ca knockout human pluripotent stem cell line via crispr cas9 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123000636 |
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