A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis
The plant cuticle is the major barrier that limits unrestricted water loss and hence plays a critical role in plant drought tolerance. Due to the presence of stomata on the leaf abaxial surface, it is technically challenging to measure abaxial cuticular transpiration. Most of the existing reports we...
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00420/full |
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| author | Yi Zhang Yi Zhang Yi Zhang Xiaobing Chen Zhenghua Du Wenjing Zhang Ananta Raj Devkota Zijian Chen Changsong Chen Weijiang Sun Mingjie Chen Mingjie Chen Mingjie Chen |
| author_facet | Yi Zhang Yi Zhang Yi Zhang Xiaobing Chen Zhenghua Du Wenjing Zhang Ananta Raj Devkota Zijian Chen Changsong Chen Weijiang Sun Mingjie Chen Mingjie Chen Mingjie Chen |
| author_sort | Yi Zhang |
| collection | DOAJ |
| description | The plant cuticle is the major barrier that limits unrestricted water loss and hence plays a critical role in plant drought tolerance. Due to the presence of stomata on the leaf abaxial surface, it is technically challenging to measure abaxial cuticular transpiration. Most of the existing reports were only focused on leaf astomatous adaxial surface, and few data are available regarding abaxial cuticular transpiration. Developing a method that can measure cuticular transpiration from both leaf surfaces simultaneously will improve our understanding about leaf transpiration barrier organization. Here, we developed a new method that enabled the simultaneous measurement of cuticular transpiration rates from the adaxial and abaxial surfaces. The proposed method combined multi-step leaf pretreatments including water equilibration under dark and ABA treatment to close stomata, as well as gum arabic or vaseline application to remove or seal the epicuticular wax layer. Mathematical formulas were established and used to calculate the transpiration rates of individual leaf surfaces from observed experimental data. This method facilitates the simultaneous quantification of cuticular transpiration from adaxial and abaxial leaf surfaces. By applying this method, we demonstrated that the adaxial intracuticular waxes and the abaxial epicuticular waxes constitute the major transpiration barriers in Camellia sinensis. Wax analysis indicated that adaxial intracuticular waxes had higher coverage of very long chain fatty acids, 1-alkanol esters, and glycols, which may be attributed to its higher transpiration barrier than that of the abaxial intracuticular waxes. |
| first_indexed | 2024-12-12T14:08:03Z |
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| id | doaj.art-334d51aeebaf4ff5a9becee9a3abe2ce |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-12T14:08:03Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-334d51aeebaf4ff5a9becee9a3abe2ce2022-12-22T00:22:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-05-011110.3389/fpls.2020.00420459126A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensisYi Zhang0Yi Zhang1Yi Zhang2Xiaobing Chen3Zhenghua Du4Wenjing Zhang5Ananta Raj Devkota6Zijian Chen7Changsong Chen8Weijiang Sun9Mingjie Chen10Mingjie Chen11Mingjie Chen12College of Horticulture and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, ChinaTea Research Institute, Fujian Academy of Agricultural Sciences, Fujian, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Engineering, University of Missouri, Columbia, MO, United StatesTea Research Institute, Fujian Academy of Agricultural Sciences, Fujian, ChinaAnxi College of Tea Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Horticulture and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaHenan Key Laboratory of Tea Plant Biology, College of Life Science, Xinyang Normal University, Xinyang, ChinaThe plant cuticle is the major barrier that limits unrestricted water loss and hence plays a critical role in plant drought tolerance. Due to the presence of stomata on the leaf abaxial surface, it is technically challenging to measure abaxial cuticular transpiration. Most of the existing reports were only focused on leaf astomatous adaxial surface, and few data are available regarding abaxial cuticular transpiration. Developing a method that can measure cuticular transpiration from both leaf surfaces simultaneously will improve our understanding about leaf transpiration barrier organization. Here, we developed a new method that enabled the simultaneous measurement of cuticular transpiration rates from the adaxial and abaxial surfaces. The proposed method combined multi-step leaf pretreatments including water equilibration under dark and ABA treatment to close stomata, as well as gum arabic or vaseline application to remove or seal the epicuticular wax layer. Mathematical formulas were established and used to calculate the transpiration rates of individual leaf surfaces from observed experimental data. This method facilitates the simultaneous quantification of cuticular transpiration from adaxial and abaxial leaf surfaces. By applying this method, we demonstrated that the adaxial intracuticular waxes and the abaxial epicuticular waxes constitute the major transpiration barriers in Camellia sinensis. Wax analysis indicated that adaxial intracuticular waxes had higher coverage of very long chain fatty acids, 1-alkanol esters, and glycols, which may be attributed to its higher transpiration barrier than that of the abaxial intracuticular waxes.https://www.frontiersin.org/article/10.3389/fpls.2020.00420/fullcuticlecuticular transpirationepicuticular waxesintracuticular waxesadaxialabaxial |
| spellingShingle | Yi Zhang Yi Zhang Yi Zhang Xiaobing Chen Zhenghua Du Wenjing Zhang Ananta Raj Devkota Zijian Chen Changsong Chen Weijiang Sun Mingjie Chen Mingjie Chen Mingjie Chen A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis Frontiers in Plant Science cuticle cuticular transpiration epicuticular waxes intracuticular waxes adaxial abaxial |
| title | A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis |
| title_full | A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis |
| title_fullStr | A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis |
| title_full_unstemmed | A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis |
| title_short | A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis |
| title_sort | proposed method for simultaneous measurement of cuticular transpiration from different leaf surfaces in camellia sinensis |
| topic | cuticle cuticular transpiration epicuticular waxes intracuticular waxes adaxial abaxial |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00420/full |
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