Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells
Human adipose-derived stem cells (hASCs) have a capacity to undergo adipogenic, chondrogenic, and osteogenic differentiation. Recently, hASCs were applied to various fields including cell therapy for tissue regeneration. However, it is hard to predict the direction of differentiation of hASCs in rea...
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2016-06-01
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author | Yoshie Arai Sunghyun Park Bogyu Choi Kyoung-Won Ko Won Chul Choi Joong-Myung Lee Dong-Wook Han Hun-Kuk Park Inbo Han Jong Hun Lee Soo-Hong Lee |
author_facet | Yoshie Arai Sunghyun Park Bogyu Choi Kyoung-Won Ko Won Chul Choi Joong-Myung Lee Dong-Wook Han Hun-Kuk Park Inbo Han Jong Hun Lee Soo-Hong Lee |
author_sort | Yoshie Arai |
collection | DOAJ |
description | Human adipose-derived stem cells (hASCs) have a capacity to undergo adipogenic, chondrogenic, and osteogenic differentiation. Recently, hASCs were applied to various fields including cell therapy for tissue regeneration. However, it is hard to predict the direction of differentiation of hASCs in real-time. Matrix metalloproteinases (MMPs) are one family of proteolytic enzymes that plays a pivotal role in regulating the biology of stem cells. MMPs secreted by hASCs are expected to show different expression patterns depending on the differentiation state of hASCs because biological functions exhibit different patterns during the differentiation of stem cells. Here, we investigated proteolytic enzyme activity, especially MMP-2 activity, in hASCs during their differentiation. The activities of proteolytic enzymes and MMP-2 were higher during chondrogenic differentiation than during adipogenic and osteogenic differentiation. During chondrogenic differentiation, mRNA expression of MMP-2 and the level of the active form of MMP-2 were increased, which also correlated with Col II. It is concluded that proteolytic enzyme activity and the level of the active form of MMP-2 were increased during chondrogenic differentiation, which was accelerated in the presence of Col II protein. According to our findings, MMP-2 could be a candidate maker for real-time detection of chondrogenic differentiation of hASCs. |
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spelling | doaj.art-334dc644fbc343549ffcb4aa94b008502022-12-22T03:09:17ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-06-0117696310.3390/ijms17060963ijms17060963Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem CellsYoshie Arai0Sunghyun Park1Bogyu Choi2Kyoung-Won Ko3Won Chul Choi4Joong-Myung Lee5Dong-Wook Han6Hun-Kuk Park7Inbo Han8Jong Hun Lee9Soo-Hong Lee10Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, KoreaDepartment of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, KoreaDepartment of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, KoreaDepartment of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, KoreaDepartment of Orthopedic Surgery, Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 443-742, KoreaDepartment of Orthopedic Surgery, Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 443-742, KoreaDepartment of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 619-961, KoreaDepartment of Biomedical Engineering, Collage of Medicine, Kyung Hee University, Seoul 151-742, KoreaDepartment of Neurosurgery, Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 443-742, KoreaDepartment of Food Science and Biotechnology, College of Life Science, CHA University, Gyeonggi-do 443-742, KoreaDepartment of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, KoreaHuman adipose-derived stem cells (hASCs) have a capacity to undergo adipogenic, chondrogenic, and osteogenic differentiation. Recently, hASCs were applied to various fields including cell therapy for tissue regeneration. However, it is hard to predict the direction of differentiation of hASCs in real-time. Matrix metalloproteinases (MMPs) are one family of proteolytic enzymes that plays a pivotal role in regulating the biology of stem cells. MMPs secreted by hASCs are expected to show different expression patterns depending on the differentiation state of hASCs because biological functions exhibit different patterns during the differentiation of stem cells. Here, we investigated proteolytic enzyme activity, especially MMP-2 activity, in hASCs during their differentiation. The activities of proteolytic enzymes and MMP-2 were higher during chondrogenic differentiation than during adipogenic and osteogenic differentiation. During chondrogenic differentiation, mRNA expression of MMP-2 and the level of the active form of MMP-2 were increased, which also correlated with Col II. It is concluded that proteolytic enzyme activity and the level of the active form of MMP-2 were increased during chondrogenic differentiation, which was accelerated in the presence of Col II protein. According to our findings, MMP-2 could be a candidate maker for real-time detection of chondrogenic differentiation of hASCs.http://www.mdpi.com/1422-0067/17/6/963human adipose-derived stem cellschondrogenic differentiationextracellular matrixmatrix metalloproteinase-2 |
spellingShingle | Yoshie Arai Sunghyun Park Bogyu Choi Kyoung-Won Ko Won Chul Choi Joong-Myung Lee Dong-Wook Han Hun-Kuk Park Inbo Han Jong Hun Lee Soo-Hong Lee Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells International Journal of Molecular Sciences human adipose-derived stem cells chondrogenic differentiation extracellular matrix matrix metalloproteinase-2 |
title | Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells |
title_full | Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells |
title_fullStr | Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells |
title_full_unstemmed | Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells |
title_short | Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells |
title_sort | enhancement of matrix metalloproteinase 2 mmp 2 as a potential chondrogenic marker during chondrogenic differentiation of human adipose derived stem cells |
topic | human adipose-derived stem cells chondrogenic differentiation extracellular matrix matrix metalloproteinase-2 |
url | http://www.mdpi.com/1422-0067/17/6/963 |
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