Effects of Shuang Dan Ming Mu Capsule on Expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, in Diabetic Retinopathy Rats

ABSTRACT: Objective The effects of Shuang Dan Ming Mu capsule on expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, were examined in a diabetic retinopathy rats model.Methods Forty Sprague-Dawley (SD) rats were randomized into Groups A (blank), B (model), C (Shuang Dan Ming Mu) and D...

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Main Authors: Jun PENG, Kun PAN, Zheng-Rong LIU, Yu-Hui QIN, Qing-Hua PENG
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2018-09-01
Series:Digital Chinese Medicine
Online Access:http://www.sciencedirect.com/science/article/pii/S2589377719300308
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author Jun PENG
Kun PAN
Zheng-Rong LIU
Yu-Hui QIN
Qing-Hua PENG
author_facet Jun PENG
Kun PAN
Zheng-Rong LIU
Yu-Hui QIN
Qing-Hua PENG
author_sort Jun PENG
collection DOAJ
description ABSTRACT: Objective The effects of Shuang Dan Ming Mu capsule on expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, were examined in a diabetic retinopathy rats model.Methods Forty Sprague-Dawley (SD) rats were randomized into Groups A (blank), B (model), C (Shuang Dan Ming Mu) and D (positive control) group, with each group containing 10 rats and 20 eyes. Rats from groups B, C and D were administered one dose of 50 mg/kg streptozotocin (STZ) by tail vein injection to establish a diabetic rat model. One week after model preparation, medication was continuously administered by gavage. After gavage for 8 weeks, the animals were sacrificed and retinal expression of VEGF-a, VEGF-b, VEGF-c and Flk-1 was quantified by immunohistochemical analysis.Results At week 8 of drug administration after model preparation, the average protein expression grayscale values for VEGF-a, VEGF-b, VEGF-c and Flk-1 in the rats model, Shuang Dan Ming Mu and positive control groups were all lower than those in the normal group, while the mean optical density values were higher than those in the normal group. When the model group was compared to the normal group, the difference was extremely significant (P < 0.01). The mean grayscale values of VEGF-a, VEGF-b, VEGF-c and Flk-1 in the Shuang Dan Ming Mu and positive control groups were all higher than those in the model group, while the mean optical density values were lower than those in the model group (P <0.05 or 0.01).Conclusion Shuang Dan Ming Mu capsule can significantly decrease the expression of VEGF-a, VEGF-b, VEGF-c and Flk-1 in the retinas of diabetic model rats and exhibit some protective effects in their retinas. Keywords: Shuang Dan Ming Mu capsule, Diabetic rats model, VEGF-a, VEGF-b, VEGF-c, Flk-1
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spelling doaj.art-334f39c9719f4720a81481e2671de65c2022-12-21T19:41:32ZengKeAi Communications Co., Ltd.Digital Chinese Medicine2589-37772018-09-0113228238Effects of Shuang Dan Ming Mu Capsule on Expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, in Diabetic Retinopathy RatsJun PENG0Kun PAN1Zheng-Rong LIU2Yu-Hui QIN3Qing-Hua PENG4Department of Traditional Chinese Medicine Ophthalmology, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaDepartment of Traditional Chinese Medicine Ophthalmology, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaDepartment of Traditional Chinese Medicine Ophthalmology, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaDepartment of Traditional Chinese Medicine Ophthalmology, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Corresponding author: Yu-Hui QIN, Professor, Research Scientist Grade II. Research direction: TCM diagnosis and treatment of fundus cornea disease. E-mail: dlqyh@sohu.com.Department of Traditional Chinese Medicine Ophthalmology, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Qing-Hua PENG, Professor Grade II. Research direction: TCM treatment and prevention of ocular fundus diseases, glaucoma and ocular surface diseases and the normalization of ophthalmic symptoms. E-mail: pqh410007@126.com. Peer review under the responsibility of Hunan University of Chinese Medicine.ABSTRACT: Objective The effects of Shuang Dan Ming Mu capsule on expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, were examined in a diabetic retinopathy rats model.Methods Forty Sprague-Dawley (SD) rats were randomized into Groups A (blank), B (model), C (Shuang Dan Ming Mu) and D (positive control) group, with each group containing 10 rats and 20 eyes. Rats from groups B, C and D were administered one dose of 50 mg/kg streptozotocin (STZ) by tail vein injection to establish a diabetic rat model. One week after model preparation, medication was continuously administered by gavage. After gavage for 8 weeks, the animals were sacrificed and retinal expression of VEGF-a, VEGF-b, VEGF-c and Flk-1 was quantified by immunohistochemical analysis.Results At week 8 of drug administration after model preparation, the average protein expression grayscale values for VEGF-a, VEGF-b, VEGF-c and Flk-1 in the rats model, Shuang Dan Ming Mu and positive control groups were all lower than those in the normal group, while the mean optical density values were higher than those in the normal group. When the model group was compared to the normal group, the difference was extremely significant (P < 0.01). The mean grayscale values of VEGF-a, VEGF-b, VEGF-c and Flk-1 in the Shuang Dan Ming Mu and positive control groups were all higher than those in the model group, while the mean optical density values were lower than those in the model group (P <0.05 or 0.01).Conclusion Shuang Dan Ming Mu capsule can significantly decrease the expression of VEGF-a, VEGF-b, VEGF-c and Flk-1 in the retinas of diabetic model rats and exhibit some protective effects in their retinas. Keywords: Shuang Dan Ming Mu capsule, Diabetic rats model, VEGF-a, VEGF-b, VEGF-c, Flk-1http://www.sciencedirect.com/science/article/pii/S2589377719300308
spellingShingle Jun PENG
Kun PAN
Zheng-Rong LIU
Yu-Hui QIN
Qing-Hua PENG
Effects of Shuang Dan Ming Mu Capsule on Expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, in Diabetic Retinopathy Rats
Digital Chinese Medicine
title Effects of Shuang Dan Ming Mu Capsule on Expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, in Diabetic Retinopathy Rats
title_full Effects of Shuang Dan Ming Mu Capsule on Expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, in Diabetic Retinopathy Rats
title_fullStr Effects of Shuang Dan Ming Mu Capsule on Expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, in Diabetic Retinopathy Rats
title_full_unstemmed Effects of Shuang Dan Ming Mu Capsule on Expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, in Diabetic Retinopathy Rats
title_short Effects of Shuang Dan Ming Mu Capsule on Expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, in Diabetic Retinopathy Rats
title_sort effects of shuang dan ming mu capsule on expression of vegf a vegf b vegf c and the vegf receptor flk 1 in diabetic retinopathy rats
url http://www.sciencedirect.com/science/article/pii/S2589377719300308
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