Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells.
Follicle-stimulating hormone (FSH) has key roles in animal reproduction, including spermatogenesis and ovarian maturation. Many factors influence FSH secretion. However, despite the broad functions of microRNAs (miRNAs) via the regulation of target genes, little is known about their roles in FSH sec...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5849326?pdf=render |
| _version_ | 1818590781044162560 |
|---|---|
| author | Dong-Xu Han Yue Xiao Chang-Jiang Wang Hao Jiang Yan Gao Bao Yuan Jia-Bao Zhang |
| author_facet | Dong-Xu Han Yue Xiao Chang-Jiang Wang Hao Jiang Yan Gao Bao Yuan Jia-Bao Zhang |
| author_sort | Dong-Xu Han |
| collection | DOAJ |
| description | Follicle-stimulating hormone (FSH) has key roles in animal reproduction, including spermatogenesis and ovarian maturation. Many factors influence FSH secretion. However, despite the broad functions of microRNAs (miRNAs) via the regulation of target genes, little is known about their roles in FSH secretion. Our previous results suggested that miR-186-5p targets the 3' UTR of FSHb; therefore, we examined whether miR-186-5p could regulate FSH secretion in rat anterior adenohypophyseal cells. miR-186-5p was transfected into rat anterior pituitary cells. The expression of FSHb and the secretion of FSH were examined by RT-qPCR and ELISA. A miR-186-5p mimic decreased the expression of FSHb compared with expression in the control group and decreased FSH secretion. In contrast, both the mRNA levels and secretion of FSH increased in response to miR-186-5p inhibitors. Our results demonstrate that miR-186-5p regulates FSH secretion by directly targeting the FSHb 3' UTR, providing additional functional evidence for the importance of miRNAs in the regulation of animal reproduction. |
| first_indexed | 2024-12-16T10:01:59Z |
| format | Article |
| id | doaj.art-3352fdc6af9f4dfba16549d9cbe10598 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T10:01:59Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3352fdc6af9f4dfba16549d9cbe105982022-12-21T22:35:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01133e019430010.1371/journal.pone.0194300Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells.Dong-Xu HanYue XiaoChang-Jiang WangHao JiangYan GaoBao YuanJia-Bao ZhangFollicle-stimulating hormone (FSH) has key roles in animal reproduction, including spermatogenesis and ovarian maturation. Many factors influence FSH secretion. However, despite the broad functions of microRNAs (miRNAs) via the regulation of target genes, little is known about their roles in FSH secretion. Our previous results suggested that miR-186-5p targets the 3' UTR of FSHb; therefore, we examined whether miR-186-5p could regulate FSH secretion in rat anterior adenohypophyseal cells. miR-186-5p was transfected into rat anterior pituitary cells. The expression of FSHb and the secretion of FSH were examined by RT-qPCR and ELISA. A miR-186-5p mimic decreased the expression of FSHb compared with expression in the control group and decreased FSH secretion. In contrast, both the mRNA levels and secretion of FSH increased in response to miR-186-5p inhibitors. Our results demonstrate that miR-186-5p regulates FSH secretion by directly targeting the FSHb 3' UTR, providing additional functional evidence for the importance of miRNAs in the regulation of animal reproduction.http://europepmc.org/articles/PMC5849326?pdf=render |
| spellingShingle | Dong-Xu Han Yue Xiao Chang-Jiang Wang Hao Jiang Yan Gao Bao Yuan Jia-Bao Zhang Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells. PLoS ONE |
| title | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells. |
| title_full | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells. |
| title_fullStr | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells. |
| title_full_unstemmed | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells. |
| title_short | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells. |
| title_sort | regulation of fsh expression by differentially expressed mir 186 5p in rat anterior adenohypophyseal cells |
| url | http://europepmc.org/articles/PMC5849326?pdf=render |
| work_keys_str_mv | AT dongxuhan regulationoffshexpressionbydifferentiallyexpressedmir1865pinratanterioradenohypophysealcells AT yuexiao regulationoffshexpressionbydifferentiallyexpressedmir1865pinratanterioradenohypophysealcells AT changjiangwang regulationoffshexpressionbydifferentiallyexpressedmir1865pinratanterioradenohypophysealcells AT haojiang regulationoffshexpressionbydifferentiallyexpressedmir1865pinratanterioradenohypophysealcells AT yangao regulationoffshexpressionbydifferentiallyexpressedmir1865pinratanterioradenohypophysealcells AT baoyuan regulationoffshexpressionbydifferentiallyexpressedmir1865pinratanterioradenohypophysealcells AT jiabaozhang regulationoffshexpressionbydifferentiallyexpressedmir1865pinratanterioradenohypophysealcells |