Molecular Identification of Protozoan <i>Sarcocystis</i> in Different Types of Water Bodies in Lithuania
Representatives of the genus <i>Sarcocystis</i> are unicellular parasites having a two-host life cycle and infecting mammals, birds, and reptiles. Until now, <i>Sarcocystis</i> spp. have been mainly investigated in definitive and intermediate hosts. Only a few studies have be...
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MDPI AG
2022-12-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/1/51 |
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| author | Agnė Baranauskaitė Živilė Strazdaitė-Žielienė Elena Servienė Dalius Butkauskas Petras Prakas |
| author_facet | Agnė Baranauskaitė Živilė Strazdaitė-Žielienė Elena Servienė Dalius Butkauskas Petras Prakas |
| author_sort | Agnė Baranauskaitė |
| collection | DOAJ |
| description | Representatives of the genus <i>Sarcocystis</i> are unicellular parasites having a two-host life cycle and infecting mammals, birds, and reptiles. Until now, <i>Sarcocystis</i> spp. have been mainly investigated in definitive and intermediate hosts. Only a few studies have been conducted on the detection of <i>Sarcocystis</i> parasites in water samples. The aim of this research was to examine whether the prevalence of <i>Sarcocystis</i> spp. parasitizing farm animals varies in different types of water bodies. Water samples (<i>n</i> = 150) were collected from the entire territory of Lithuania, dividing water bodies into five groups (lakes, rivers, ponds/canals, swamps, and the inshore zone of the territorial Baltic Sea area). One-liter samples were filtered and subsequently analyzed using nested PCR. At least one of the analyzed <i>Sarcocystis</i> spp. (<i>S</i>. <i>arieticanis</i>, <i>S</i>. <i>bertrami</i>, <i>S</i>. <i>bovifelis</i>, <i>S</i>. <i>capracanis</i>, <i>S</i>. <i>cruzi</i>, <i>S</i>. <i>hirsuta</i>, <i>S</i>. <i>miescheriana</i>, and <i>S</i>. <i>tenella</i>) was determined in all examined samples from water bodies. No significant difference in <i>Sarcocystis</i> spp. prevalence between different types of water sources was detected. Our research proved that selecting appropriate primers is important for the accurate identification of parasites in samples collected from water bodies. |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T11:57:23Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-3353135ab18545e294e439ebb23b19392023-11-30T23:07:18ZengMDPI AGLife2075-17292022-12-011315110.3390/life13010051Molecular Identification of Protozoan <i>Sarcocystis</i> in Different Types of Water Bodies in LithuaniaAgnė Baranauskaitė0Živilė Strazdaitė-Žielienė1Elena Servienė2Dalius Butkauskas3Petras Prakas4Nature Research Centre, Akademijos Str. 2, 08412 Vilnius, LithuaniaNature Research Centre, Akademijos Str. 2, 08412 Vilnius, LithuaniaNature Research Centre, Akademijos Str. 2, 08412 Vilnius, LithuaniaNature Research Centre, Akademijos Str. 2, 08412 Vilnius, LithuaniaNature Research Centre, Akademijos Str. 2, 08412 Vilnius, LithuaniaRepresentatives of the genus <i>Sarcocystis</i> are unicellular parasites having a two-host life cycle and infecting mammals, birds, and reptiles. Until now, <i>Sarcocystis</i> spp. have been mainly investigated in definitive and intermediate hosts. Only a few studies have been conducted on the detection of <i>Sarcocystis</i> parasites in water samples. The aim of this research was to examine whether the prevalence of <i>Sarcocystis</i> spp. parasitizing farm animals varies in different types of water bodies. Water samples (<i>n</i> = 150) were collected from the entire territory of Lithuania, dividing water bodies into five groups (lakes, rivers, ponds/canals, swamps, and the inshore zone of the territorial Baltic Sea area). One-liter samples were filtered and subsequently analyzed using nested PCR. At least one of the analyzed <i>Sarcocystis</i> spp. (<i>S</i>. <i>arieticanis</i>, <i>S</i>. <i>bertrami</i>, <i>S</i>. <i>bovifelis</i>, <i>S</i>. <i>capracanis</i>, <i>S</i>. <i>cruzi</i>, <i>S</i>. <i>hirsuta</i>, <i>S</i>. <i>miescheriana</i>, and <i>S</i>. <i>tenella</i>) was determined in all examined samples from water bodies. No significant difference in <i>Sarcocystis</i> spp. prevalence between different types of water sources was detected. Our research proved that selecting appropriate primers is important for the accurate identification of parasites in samples collected from water bodies.https://www.mdpi.com/2075-1729/13/1/51environment<i>Sarcocystis</i> speciesmolecular detection of parasiteswater samples |
| spellingShingle | Agnė Baranauskaitė Živilė Strazdaitė-Žielienė Elena Servienė Dalius Butkauskas Petras Prakas Molecular Identification of Protozoan <i>Sarcocystis</i> in Different Types of Water Bodies in Lithuania Life environment <i>Sarcocystis</i> species molecular detection of parasites water samples |
| title | Molecular Identification of Protozoan <i>Sarcocystis</i> in Different Types of Water Bodies in Lithuania |
| title_full | Molecular Identification of Protozoan <i>Sarcocystis</i> in Different Types of Water Bodies in Lithuania |
| title_fullStr | Molecular Identification of Protozoan <i>Sarcocystis</i> in Different Types of Water Bodies in Lithuania |
| title_full_unstemmed | Molecular Identification of Protozoan <i>Sarcocystis</i> in Different Types of Water Bodies in Lithuania |
| title_short | Molecular Identification of Protozoan <i>Sarcocystis</i> in Different Types of Water Bodies in Lithuania |
| title_sort | molecular identification of protozoan i sarcocystis i in different types of water bodies in lithuania |
| topic | environment <i>Sarcocystis</i> species molecular detection of parasites water samples |
| url | https://www.mdpi.com/2075-1729/13/1/51 |
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