Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis)
Sepia pharaonis is an excellent candidate for aquaculture in China. However, the low survival rate during early feed transition is a bottleneck restricting industrial development. Understanding the changes in digestive physiology and intestinal microflora during feed transition should enable us to m...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.941488/full |
| _version_ | 1828108957364781056 |
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| author | Maowang Jiang Wencheng Xiao Jingtao Ye Liting Xu Ruibing Peng Qingxi Han Zhenming Lü Huilai Shi Xiamin Jiang |
| author_facet | Maowang Jiang Wencheng Xiao Jingtao Ye Liting Xu Ruibing Peng Qingxi Han Zhenming Lü Huilai Shi Xiamin Jiang |
| author_sort | Maowang Jiang |
| collection | DOAJ |
| description | Sepia pharaonis is an excellent candidate for aquaculture in China. However, the low survival rate during early feed transition is a bottleneck restricting industrial development. Understanding the changes in digestive physiology and intestinal microflora during feed transition should enable us to meet their nutritional needs to improve production. In this study, we investigate the digestive enzyme of S. pharaonis and undertake histological observations of the digestive gland and intestine. The intestinal microflora 16S rRNA genes were also analyzed using high-throughput sequencing of the pre, mid, and post-feed transition stages (20, 40, and 60 days post-hatching (DPH), respectively). The digestive enzymes from the digestive gland (trypsin and chymotrypsin) rapidly decrease at 40 DPH when compared to their levels at 20 DPH, but mostly recovered by 60 DPH. The alkaline phosphatase and lipase increased sharply by 40 DPH, then peaked at 60 DPH. The intestinal digestive enzymes followed similar trends during feed transition, except for lipase activity, which decreased after 20 DPH and remained low, even at 60 DPH. Feed transition affects the morphogenesis of the digestive tract and feed transition stress leads to the impairment of the digestive gland and intestinal morphology, which reduces the digestive capacity, but almost totally recovers by 60 DPH. Moreover, the comparison of the intestinal microbial composition during feed transition revealed that the dominant phylum Bacteroidetes gradually increased to a peak at 40 DPH and then decreased until 60 DPH. The microbial composition changed with the most abundant genus Pseudomonas being replaced by Acinetobacter. The phylum and family level investigation suggested the microbiota in the rearing water had limited influence on the intestinal microbiota. The intestinal microbiota diversity increased during feed transition. This study improves our understanding of changes and adaptations in cuttlefish during feed transition. |
| first_indexed | 2024-04-11T10:56:14Z |
| format | Article |
| id | doaj.art-335460bd6f8b45c2bf3f0ac5fe584246 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-11T10:56:14Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-335460bd6f8b45c2bf3f0ac5fe5842462022-12-22T04:28:46ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-09-01910.3389/fmars.2022.941488941488Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis)Maowang Jiang0Wencheng Xiao1Jingtao Ye2Liting Xu3Ruibing Peng4Qingxi Han5Zhenming Lü6Huilai Shi7Xiamin Jiang8Key Laboratory of Applied Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo, ChinaKey Laboratory of Applied Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo, ChinaKey Laboratory of Applied Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo, ChinaKey Laboratory of Applied Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo, ChinaKey Laboratory of Applied Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo, ChinaKey Laboratory of Applied Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo, ChinaNational Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan, ChinaMariculture Research Department, Marine Fisheries Research Institute of Zhejiang Province, Zhoushan, ChinaKey Laboratory of Applied Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo, ChinaSepia pharaonis is an excellent candidate for aquaculture in China. However, the low survival rate during early feed transition is a bottleneck restricting industrial development. Understanding the changes in digestive physiology and intestinal microflora during feed transition should enable us to meet their nutritional needs to improve production. In this study, we investigate the digestive enzyme of S. pharaonis and undertake histological observations of the digestive gland and intestine. The intestinal microflora 16S rRNA genes were also analyzed using high-throughput sequencing of the pre, mid, and post-feed transition stages (20, 40, and 60 days post-hatching (DPH), respectively). The digestive enzymes from the digestive gland (trypsin and chymotrypsin) rapidly decrease at 40 DPH when compared to their levels at 20 DPH, but mostly recovered by 60 DPH. The alkaline phosphatase and lipase increased sharply by 40 DPH, then peaked at 60 DPH. The intestinal digestive enzymes followed similar trends during feed transition, except for lipase activity, which decreased after 20 DPH and remained low, even at 60 DPH. Feed transition affects the morphogenesis of the digestive tract and feed transition stress leads to the impairment of the digestive gland and intestinal morphology, which reduces the digestive capacity, but almost totally recovers by 60 DPH. Moreover, the comparison of the intestinal microbial composition during feed transition revealed that the dominant phylum Bacteroidetes gradually increased to a peak at 40 DPH and then decreased until 60 DPH. The microbial composition changed with the most abundant genus Pseudomonas being replaced by Acinetobacter. The phylum and family level investigation suggested the microbiota in the rearing water had limited influence on the intestinal microbiota. The intestinal microbiota diversity increased during feed transition. This study improves our understanding of changes and adaptations in cuttlefish during feed transition.https://www.frontiersin.org/articles/10.3389/fmars.2022.941488/fulldigestive tractdigestive enzymehistologymicrobiotacuttlefishfeed transition |
| spellingShingle | Maowang Jiang Wencheng Xiao Jingtao Ye Liting Xu Ruibing Peng Qingxi Han Zhenming Lü Huilai Shi Xiamin Jiang Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis) Frontiers in Marine Science digestive tract digestive enzyme histology microbiota cuttlefish feed transition |
| title | Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis) |
| title_full | Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis) |
| title_fullStr | Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis) |
| title_full_unstemmed | Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis) |
| title_short | Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis) |
| title_sort | effects of feed transition on digestive tract digestive enzyme morphology and intestinal community in cuttlefish sepia pharaonis |
| topic | digestive tract digestive enzyme histology microbiota cuttlefish feed transition |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.941488/full |
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