Introgression of bacterial leaf blight (BLB) resistant gene, Xa7 into MARDI elite variety, MR219 by marker assisted backcrossing (MABC) approach
Abstract Bacterial leaf blight (BLB) is one of the major rice diseases in Malaysia. This disease causes substantial yield loss as high as 70%. Development of rice varieties which inherited BLB resistant traits is a crucial approach to promote and sustain rice industry in Malaysia. Hence, this study...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-11-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100111&tlng=en |
| _version_ | 1798015089896325120 |
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| author | S. N. Yazid K. Ahmad M. S. F. A. Razak Z. A. Rahman K. Ramachandran S. N. A. Mohamad M. B. AB Ghaffar |
| author_facet | S. N. Yazid K. Ahmad M. S. F. A. Razak Z. A. Rahman K. Ramachandran S. N. A. Mohamad M. B. AB Ghaffar |
| author_sort | S. N. Yazid |
| collection | DOAJ |
| description | Abstract Bacterial leaf blight (BLB) is one of the major rice diseases in Malaysia. This disease causes substantial yield loss as high as 70%. Development of rice varieties which inherited BLB resistant traits is a crucial approach to promote and sustain rice industry in Malaysia. Hence, this study aims were to enhance BLB disease resistant characters of high yielding commercial variety MR219 through backcross breeding approach with supporting tool of marker-assisted selection (MAS). Broad spectrum BLB resistance gene, Xa7 from donor parent IRBB7 were introgressed into the susceptible MR219 (recurrent parent) using two flanking markers ID7 and ID15. At BC3F4, we managed to generate 19 introgressed lines with homozygous Xa7 gene and showed resistant characteristics as donor parent when it was challenged with Xanthomonas oryzae pv. oryzae through artificial inoculation. Recurrent parent MR219 and control variety, MR263 were found to be severely infected by the disease. The improved lines exhibited similar morphological and yield performance characters as to the elite variety, MR219. Two lines, PB-2-107 and PB-2-34 were chosen to be potential lines because of their outstanding performances compared to parent, MR219. This study demonstrates a success story of MAS application in development of improved disease resistance lines of rice against BLB disease. |
| first_indexed | 2024-04-11T15:28:49Z |
| format | Article |
| id | doaj.art-335c4875a86d4b958c433c42c5ea5c95 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-11T15:28:49Z |
| publishDate | 2021-11-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-335c4875a86d4b958c433c42c5ea5c952022-12-22T04:16:11ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-11-018410.1590/1519-6984.248359Introgression of bacterial leaf blight (BLB) resistant gene, Xa7 into MARDI elite variety, MR219 by marker assisted backcrossing (MABC) approachS. N. Yazidhttps://orcid.org/0000-0003-3604-5123K. Ahmadhttps://orcid.org/0000-0002-4296-3425M. S. F. A. RazakZ. A. Rahmanhttps://orcid.org/0000-0002-3249-2690K. Ramachandranhttps://orcid.org/0000-0002-3203-7575S. N. A. Mohamadhttps://orcid.org/0000-0001-7704-3664M. B. AB Ghaffarhttps://orcid.org/0000-0002-6099-8779Abstract Bacterial leaf blight (BLB) is one of the major rice diseases in Malaysia. This disease causes substantial yield loss as high as 70%. Development of rice varieties which inherited BLB resistant traits is a crucial approach to promote and sustain rice industry in Malaysia. Hence, this study aims were to enhance BLB disease resistant characters of high yielding commercial variety MR219 through backcross breeding approach with supporting tool of marker-assisted selection (MAS). Broad spectrum BLB resistance gene, Xa7 from donor parent IRBB7 were introgressed into the susceptible MR219 (recurrent parent) using two flanking markers ID7 and ID15. At BC3F4, we managed to generate 19 introgressed lines with homozygous Xa7 gene and showed resistant characteristics as donor parent when it was challenged with Xanthomonas oryzae pv. oryzae through artificial inoculation. Recurrent parent MR219 and control variety, MR263 were found to be severely infected by the disease. The improved lines exhibited similar morphological and yield performance characters as to the elite variety, MR219. Two lines, PB-2-107 and PB-2-34 were chosen to be potential lines because of their outstanding performances compared to parent, MR219. This study demonstrates a success story of MAS application in development of improved disease resistance lines of rice against BLB disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100111&tlng=enOryza sativa L.backcrossingmarker assisted selectionXa7 genebacterial leaf blight |
| spellingShingle | S. N. Yazid K. Ahmad M. S. F. A. Razak Z. A. Rahman K. Ramachandran S. N. A. Mohamad M. B. AB Ghaffar Introgression of bacterial leaf blight (BLB) resistant gene, Xa7 into MARDI elite variety, MR219 by marker assisted backcrossing (MABC) approach Brazilian Journal of Biology Oryza sativa L. backcrossing marker assisted selection Xa7 gene bacterial leaf blight |
| title | Introgression of bacterial leaf blight (BLB) resistant gene, Xa7 into MARDI elite variety, MR219 by marker assisted backcrossing (MABC) approach |
| title_full | Introgression of bacterial leaf blight (BLB) resistant gene, Xa7 into MARDI elite variety, MR219 by marker assisted backcrossing (MABC) approach |
| title_fullStr | Introgression of bacterial leaf blight (BLB) resistant gene, Xa7 into MARDI elite variety, MR219 by marker assisted backcrossing (MABC) approach |
| title_full_unstemmed | Introgression of bacterial leaf blight (BLB) resistant gene, Xa7 into MARDI elite variety, MR219 by marker assisted backcrossing (MABC) approach |
| title_short | Introgression of bacterial leaf blight (BLB) resistant gene, Xa7 into MARDI elite variety, MR219 by marker assisted backcrossing (MABC) approach |
| title_sort | introgression of bacterial leaf blight blb resistant gene xa7 into mardi elite variety mr219 by marker assisted backcrossing mabc approach |
| topic | Oryza sativa L. backcrossing marker assisted selection Xa7 gene bacterial leaf blight |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100111&tlng=en |
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