Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data
Introduction. Influenza is a severe viral disease, a frequent complication of which is a secondary bacterial pneumonia. Influenza vaccines prevent secondary bacterial complications. Virus-like particles are one of the promising areas for the development of new vaccines. The aim of this work is to s...
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Central Research Institute for Epidemiology
2020-07-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/388/262 |
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| author | I. N. Falynskova A. Yu. Egorov A. V. Poddubikov N. O. Vartanova N. P. Kartashova E. A. Glubokova V. A. Mkhitarov D. S. Dzhalilova O. V. Makarova I. A. Leneva |
| author_facet | I. N. Falynskova A. Yu. Egorov A. V. Poddubikov N. O. Vartanova N. P. Kartashova E. A. Glubokova V. A. Mkhitarov D. S. Dzhalilova O. V. Makarova I. A. Leneva |
| author_sort | I. N. Falynskova |
| collection | DOAJ |
| description | Introduction. Influenza is a severe viral disease, a frequent complication of which is a secondary bacterial pneumonia. Influenza vaccines prevent secondary bacterial complications. Virus-like particles are one of the promising areas for the development of new vaccines.
The aim of this work is to study the correlation of the pathomorphological characteristics of the lungs with clinical, virological, and microbiological markers of the disease at vaccination with virus-like particles (VLPs), containing hemagglutinin (HA) of influenza virus (HA-Gag-VLPs) in a murine model of secondary bacterial pneumonia induced by S. pneumoniae after influenza infection.
Material and methods. BALB/c mice were vaccinated with VLPs containing influenza HA. After 21 days, mice were infected with two strains of influenza viruses, homologous and non-homologous, and 5 days after viral infection, were infected with S. pneumoniae. The vaccination effect was evaluated by morphological, virological (titer of the virus in the lungs) and microbiological (titer of bacteria in the lungs) data, and was confirmed by clinical data (survival, change in body weight).
Results. Immunization with HA-Gag-VLPs, followed by infection with a homologous influenza virus and S. pneumoniae, reduced the area of foci of inflammation, inhibited the replication of the virus and bacteria in the lungs, and also protected animals from death and reduced their weight loss. Immunization with HA-Gag-VLPs upon infection with a heterologous strain and S. pneumoniae did not affect these criteria.
Conclusion. The immunization with HA-Gag-VLPs prevented the viral replication, providing a reduction of S. pneumoniae titer and the degree of lung damage, protecting animals from the disease in a murine model of secondary bacterial pneumonia, induced by S. pneumoniae, after influenza infection with homologous strain of the virus. |
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| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:28Z |
| publishDate | 2020-07-01 |
| publisher | Central Research Institute for Epidemiology |
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| series | Вопросы вирусологии |
| spelling | doaj.art-336733994bfa484081ce9e07daabbd5a2023-07-12T19:57:43ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972020-07-0165315015810.36233/0507-4088-2020-65-3-150-158338Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical dataI. N. Falynskova0https://orcid.org/0000-0001-9836-9620A. Yu. Egorov1https://orcid.org/0000-0003-2068-3745A. V. Poddubikov2https://orcid.org/0000-0001-8962-4765N. O. Vartanova3https://orcid.org/0000-0002-6372-9910N. P. Kartashova4https://orcid.org/0000-0003-2096-5080E. A. Glubokova5https://orcid.org/0000-0002-5925-9733V. A. Mkhitarov6https://orcid.org/0000-0002-4427-1991D. S. Dzhalilova7https://orcid.org/0000-0002-1337-7160O. V. Makarova8https://orcid.org/0000-0001-8581-107XI. A. Leneva9https://orcid.org/0000-0002-7755-2714I.I. Mechnikov Research Institute of Vaccines and SeraI.I. Mechnikov Research Institute of Vaccines and SeraI.I. Mechnikov Research Institute of Vaccines and SeraI.I. Mechnikov Research Institute of Vaccines and SeraI.I. Mechnikov Research Institute of Vaccines and SeraI.I. Mechnikov Research Institute of Vaccines and SeraResearch Institute of Human MorphologyResearch Institute of Human MorphologyResearch Institute of Human MorphologyI.I. Mechnikov Research Institute of Vaccines and SeraIntroduction. Influenza is a severe viral disease, a frequent complication of which is a secondary bacterial pneumonia. Influenza vaccines prevent secondary bacterial complications. Virus-like particles are one of the promising areas for the development of new vaccines. The aim of this work is to study the correlation of the pathomorphological characteristics of the lungs with clinical, virological, and microbiological markers of the disease at vaccination with virus-like particles (VLPs), containing hemagglutinin (HA) of influenza virus (HA-Gag-VLPs) in a murine model of secondary bacterial pneumonia induced by S. pneumoniae after influenza infection. Material and methods. BALB/c mice were vaccinated with VLPs containing influenza HA. After 21 days, mice were infected with two strains of influenza viruses, homologous and non-homologous, and 5 days after viral infection, were infected with S. pneumoniae. The vaccination effect was evaluated by morphological, virological (titer of the virus in the lungs) and microbiological (titer of bacteria in the lungs) data, and was confirmed by clinical data (survival, change in body weight). Results. Immunization with HA-Gag-VLPs, followed by infection with a homologous influenza virus and S. pneumoniae, reduced the area of foci of inflammation, inhibited the replication of the virus and bacteria in the lungs, and also protected animals from death and reduced their weight loss. Immunization with HA-Gag-VLPs upon infection with a heterologous strain and S. pneumoniae did not affect these criteria. Conclusion. The immunization with HA-Gag-VLPs prevented the viral replication, providing a reduction of S. pneumoniae titer and the degree of lung damage, protecting animals from the disease in a murine model of secondary bacterial pneumonia, induced by S. pneumoniae, after influenza infection with homologous strain of the virus.https://virusjour.crie.ru/jour/article/viewFile/388/262influenza virusstreptococcus pneumoniaevlpsecondary bacterial pneumonia after influenza infection |
| spellingShingle | I. N. Falynskova A. Yu. Egorov A. V. Poddubikov N. O. Vartanova N. P. Kartashova E. A. Glubokova V. A. Mkhitarov D. S. Dzhalilova O. V. Makarova I. A. Leneva Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data Вопросы вирусологии influenza virus streptococcus pneumoniae vlp secondary bacterial pneumonia after influenza infection |
| title | Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data |
| title_full | Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data |
| title_fullStr | Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data |
| title_full_unstemmed | Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data |
| title_short | Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data |
| title_sort | vaccination with virus like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia virological microbiological and clinical data |
| topic | influenza virus streptococcus pneumoniae vlp secondary bacterial pneumonia after influenza infection |
| url | https://virusjour.crie.ru/jour/article/viewFile/388/262 |
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