Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018
A novel avian influenza virus (A/wild duck/Korea/K102/2018) (H2N9) was isolated from wild birds in South Korea in 2018, and phylogenetic and molecular analyses were conducted on complete gene sequences obtained by next-generation sequencing. Phylogenetic analysis indicated that the hemagglutinin (HA...
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MDPI AG
2019-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/11/1046 |
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| author | Seon-Ju Yeo Duc-Duong Than Hong-Seog Park Haan Woo Sung Hyun Park |
| author_facet | Seon-Ju Yeo Duc-Duong Than Hong-Seog Park Haan Woo Sung Hyun Park |
| author_sort | Seon-Ju Yeo |
| collection | DOAJ |
| description | A novel avian influenza virus (A/wild duck/Korea/K102/2018) (H2N9) was isolated from wild birds in South Korea in 2018, and phylogenetic and molecular analyses were conducted on complete gene sequences obtained by next-generation sequencing. Phylogenetic analysis indicated that the hemagglutinin (HA) and neuraminidase (NA) genes of the A/wild duck/Korea/K102/2018 (H2N9) virus belonged to the Eurasian countries, whereas other internal genes (polymerase basic protein 1 (PB1), PB2, nucleoprotein (NP), polymerase acidic protein (PA), matrix protein (M), and non-structural protein (NS)) belonged to the East Asian countries. A monobasic amino acid (PQIEPR/GLF) at the HA cleavage site, E627 in the PB2 gene, and no deletion of the stalk region in the NA gene indicated that the A/wild duck/Korea/K102/2018 (H2N9) isolate was a typical low pathogenicity avian influenza (LPAI). Nucleotide sequence similarity analysis of HA revealed that the highest homology (98.34%) is to that of A/duck/Mongolia/482/2015 (H2N3), and amino acid sequence of NA was closely related to that of A/duck/Bangladesh/8987/2010 (H10N9) (96.45%). In contrast, internal genes showed homology higher than 98% compared to those of other isolates derived from duck and wild birds of China or Japan in 2016−2018. The newly isolated A/wild duck/Korea/K102/2018 (H2N9) strain is the first reported avian influenza virus in Korea, and may have evolved from multiple genotypes in wild birds and ducks in Mongolia, China, and Japan. |
| first_indexed | 2024-12-18T08:13:58Z |
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| language | English |
| last_indexed | 2024-12-18T08:13:58Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
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| series | Viruses |
| spelling | doaj.art-3367c782f5db40a5b9340fc4543807312022-12-21T21:14:50ZengMDPI AGViruses1999-49152019-11-011111104610.3390/v11111046v11111046Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018Seon-Ju Yeo0Duc-Duong Than1Hong-Seog Park2Haan Woo Sung3Hyun Park4Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan 54538, KoreaZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan 54538, KoreaGnCBio Inc, 4F, Yegan Plaza, 36, Banseok-ro, Yuseong-gu, Daejeon 34069, KoreaCollege of Veterinary Medicine, Kangwon National University, Chuncheon 24341, KoreaZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan 54538, KoreaA novel avian influenza virus (A/wild duck/Korea/K102/2018) (H2N9) was isolated from wild birds in South Korea in 2018, and phylogenetic and molecular analyses were conducted on complete gene sequences obtained by next-generation sequencing. Phylogenetic analysis indicated that the hemagglutinin (HA) and neuraminidase (NA) genes of the A/wild duck/Korea/K102/2018 (H2N9) virus belonged to the Eurasian countries, whereas other internal genes (polymerase basic protein 1 (PB1), PB2, nucleoprotein (NP), polymerase acidic protein (PA), matrix protein (M), and non-structural protein (NS)) belonged to the East Asian countries. A monobasic amino acid (PQIEPR/GLF) at the HA cleavage site, E627 in the PB2 gene, and no deletion of the stalk region in the NA gene indicated that the A/wild duck/Korea/K102/2018 (H2N9) isolate was a typical low pathogenicity avian influenza (LPAI). Nucleotide sequence similarity analysis of HA revealed that the highest homology (98.34%) is to that of A/duck/Mongolia/482/2015 (H2N3), and amino acid sequence of NA was closely related to that of A/duck/Bangladesh/8987/2010 (H10N9) (96.45%). In contrast, internal genes showed homology higher than 98% compared to those of other isolates derived from duck and wild birds of China or Japan in 2016−2018. The newly isolated A/wild duck/Korea/K102/2018 (H2N9) strain is the first reported avian influenza virus in Korea, and may have evolved from multiple genotypes in wild birds and ducks in Mongolia, China, and Japan.https://www.mdpi.com/1999-4915/11/11/1046novel avian influenza virus isolateh2n9koreawild duck |
| spellingShingle | Seon-Ju Yeo Duc-Duong Than Hong-Seog Park Haan Woo Sung Hyun Park Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018 Viruses novel avian influenza virus isolate h2n9 korea wild duck |
| title | Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018 |
| title_full | Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018 |
| title_fullStr | Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018 |
| title_full_unstemmed | Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018 |
| title_short | Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018 |
| title_sort | molecular characterization of a novel avian influenza a h2n9 strain isolated from wild duck in korea in 2018 |
| topic | novel avian influenza virus isolate h2n9 korea wild duck |
| url | https://www.mdpi.com/1999-4915/11/11/1046 |
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