Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New Caledonia
Dengue virus infection is endemic in New Caledonia, with outbreaks occurring every year. We evaluated the Biosynex<sup>®</sup> Dengue NS1 antigen rapid diagnostic test (RDT) for the early diagnosis of dengue in patients attending a local hospital in northern New Caledonia. Samples collec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/346 |
| _version_ | 1827653707432460288 |
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| author | Enagnon Kazali Alidjinou Sylvie Tardieu Isabelle Vrenken Didier Hober Ann-Claire Gourinat |
| author_facet | Enagnon Kazali Alidjinou Sylvie Tardieu Isabelle Vrenken Didier Hober Ann-Claire Gourinat |
| author_sort | Enagnon Kazali Alidjinou |
| collection | DOAJ |
| description | Dengue virus infection is endemic in New Caledonia, with outbreaks occurring every year. We evaluated the Biosynex<sup>®</sup> Dengue NS1 antigen rapid diagnostic test (RDT) for the early diagnosis of dengue in patients attending a local hospital in northern New Caledonia. Samples collected from patients suspected of dengue infection were tested with RDT at the local laboratory, and then sent to the reference laboratory for confirmation with real-time RT-PCR. A total of 472 samples were included during the study period. RT-PCR yielded a positive result in 154 samples (32.6%). The sensitivity and specificity of the NS1 antigen RDT were 79.9% and 96.2%, respectively. The performance of the RDT varied by the time of sampling and dengue virus serotype. In conclusion, Biosynex<sup>®</sup> Dengue NS1 antigen RDT showed a sensitivity and a specificity in the upper range usually reported for this type of test. Several factors can lead to a suboptimal sensitivity, and negative samples with suggestive clinical features should be retested with reference methods. |
| first_indexed | 2024-03-09T21:23:29Z |
| format | Article |
| id | doaj.art-3369f683d246493aaa5d0b0c67731d83 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:29Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3369f683d246493aaa5d0b0c67731d832023-11-23T21:14:40ZengMDPI AGMicroorganisms2076-26072022-02-0110234610.3390/microorganisms10020346Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New CaledoniaEnagnon Kazali Alidjinou0Sylvie Tardieu1Isabelle Vrenken2Didier Hober3Ann-Claire Gourinat4Laboratoire de Virologie ULR3610, University of Lille, CHU Lille, F-59000 Lille, FranceLaboratory Department, Centre Hospitalier du Nord, 98860 Kone, FranceLaboratory Department, Centre Hospitalier du Nord, 98860 Kone, FranceLaboratoire de Virologie ULR3610, University of Lille, CHU Lille, F-59000 Lille, FranceMicrobiology Laboratory, Centre Hospitalier Territorial de Nouvelle-Calédonie, 98835 Dumbea, FranceDengue virus infection is endemic in New Caledonia, with outbreaks occurring every year. We evaluated the Biosynex<sup>®</sup> Dengue NS1 antigen rapid diagnostic test (RDT) for the early diagnosis of dengue in patients attending a local hospital in northern New Caledonia. Samples collected from patients suspected of dengue infection were tested with RDT at the local laboratory, and then sent to the reference laboratory for confirmation with real-time RT-PCR. A total of 472 samples were included during the study period. RT-PCR yielded a positive result in 154 samples (32.6%). The sensitivity and specificity of the NS1 antigen RDT were 79.9% and 96.2%, respectively. The performance of the RDT varied by the time of sampling and dengue virus serotype. In conclusion, Biosynex<sup>®</sup> Dengue NS1 antigen RDT showed a sensitivity and a specificity in the upper range usually reported for this type of test. Several factors can lead to a suboptimal sensitivity, and negative samples with suggestive clinical features should be retested with reference methods.https://www.mdpi.com/2076-2607/10/2/346dengue virusNS1 antigenrapid diagnostic testRT-PCRevaluation |
| spellingShingle | Enagnon Kazali Alidjinou Sylvie Tardieu Isabelle Vrenken Didier Hober Ann-Claire Gourinat Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New Caledonia Microorganisms dengue virus NS1 antigen rapid diagnostic test RT-PCR evaluation |
| title | Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New Caledonia |
| title_full | Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New Caledonia |
| title_fullStr | Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New Caledonia |
| title_full_unstemmed | Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New Caledonia |
| title_short | Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New Caledonia |
| title_sort | prospective evaluation of a commercial dengue ns1 antigen rapid diagnostic test in new caledonia |
| topic | dengue virus NS1 antigen rapid diagnostic test RT-PCR evaluation |
| url | https://www.mdpi.com/2076-2607/10/2/346 |
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