Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems
Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region (Russia) were analyzed. A low level of chromosomal polymorphism has been confirmed, eventually eight banding sequences were found: melA1, melB1, melC1, melD1, melE1, melF1, and melG1; only melD2 was found i...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2022-09-01
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| Series: | Comparative Cytogenetics |
| Online Access: | https://compcytogen.pensoft.net/article/90336/download/pdf/ |
| _version_ | 1811261545658712064 |
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| author | Viktor V. Bolshakov Ekaterina A. Movergoz |
| author_facet | Viktor V. Bolshakov Ekaterina A. Movergoz |
| author_sort | Viktor V. Bolshakov |
| collection | DOAJ |
| description | Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region (Russia) were analyzed. A low level of chromosomal polymorphism has been confirmed, eventually eight banding sequences were found: melA1, melB1, melC1, melD1, melE1, melF1, and melG1; only melD2 was found in two larvae from the Sunoga river. Analysis of phylogenetic tree and estimated genetic distances has shown not all COI gene sequences of Ch. melanotus in GenBank and BOLD to belong to this species. The lower distance of 0.4% was observed between two sequences from the Yaroslavl region and Finland, apparently these are true Ch. melanotus sequences. The distances between true Ch. melanotus and other sequences from Finland were 9.5% and 12.4%, and from Sweden it was 11%. The average genetic distance between studied sequences of 9.1% is out of the range of the 3% threshold previously determined for chironomids. According to our estimates, there are two sequences with a distance of 2.9% that may belong to Ch. annularius Meigen, 1818, and one sequence with a genetic distance of 2.1%, may belonging to Ch. cingulatus Meigen, 1830, which has been confirmed karyologically. Another two sequences form a separate cluster. We suggest that they either belong to a known species, but are not present in the databases, or belong to a distinct, undescribed species. |
| first_indexed | 2024-04-12T19:06:26Z |
| format | Article |
| id | doaj.art-336cd427a8e84d3a92c9477bdd4b5e98 |
| institution | Directory Open Access Journal |
| issn | 1993-078X |
| language | English |
| last_indexed | 2024-04-12T19:06:26Z |
| publishDate | 2022-09-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Comparative Cytogenetics |
| spelling | doaj.art-336cd427a8e84d3a92c9477bdd4b5e982022-12-22T03:20:00ZengPensoft PublishersComparative Cytogenetics1993-078X2022-09-0116316117210.3897/compcytogen.v16.i3.9033690336Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systemsViktor V. Bolshakov0Ekaterina A. Movergoz1Papanin Institute for Biology of Inland Waters Russian Academy of SciencesPapanin Institute for Biology of Inland Waters Russian Academy of SciencesKaryotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region (Russia) were analyzed. A low level of chromosomal polymorphism has been confirmed, eventually eight banding sequences were found: melA1, melB1, melC1, melD1, melE1, melF1, and melG1; only melD2 was found in two larvae from the Sunoga river. Analysis of phylogenetic tree and estimated genetic distances has shown not all COI gene sequences of Ch. melanotus in GenBank and BOLD to belong to this species. The lower distance of 0.4% was observed between two sequences from the Yaroslavl region and Finland, apparently these are true Ch. melanotus sequences. The distances between true Ch. melanotus and other sequences from Finland were 9.5% and 12.4%, and from Sweden it was 11%. The average genetic distance between studied sequences of 9.1% is out of the range of the 3% threshold previously determined for chironomids. According to our estimates, there are two sequences with a distance of 2.9% that may belong to Ch. annularius Meigen, 1818, and one sequence with a genetic distance of 2.1%, may belonging to Ch. cingulatus Meigen, 1830, which has been confirmed karyologically. Another two sequences form a separate cluster. We suggest that they either belong to a known species, but are not present in the databases, or belong to a distinct, undescribed species.https://compcytogen.pensoft.net/article/90336/download/pdf/ |
| spellingShingle | Viktor V. Bolshakov Ekaterina A. Movergoz Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems Comparative Cytogenetics |
| title | Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems |
| title_full | Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems |
| title_fullStr | Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems |
| title_full_unstemmed | Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems |
| title_short | Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems |
| title_sort | karyotype and coi gene sequences of chironomus melanotus keyl 1961 from the yaroslavl region russia and the difficulties with its identification using genbank and bold systems |
| url | https://compcytogen.pensoft.net/article/90336/download/pdf/ |
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