Porphyromonas gingivalis: a clonal pathogen?
The introduction of multilocus sequence typing (MLST) in infectious disease research has allowed standardized typing of bacterial clones. Through multiple markers around the genome, it is possible to determine the sequence type (ST) of bacterial isolates to establish the population structure of a sp...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2011-11-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | http://www.journaloforalmicrobiology.net/index.php/jom/article/view/8487/16563 |
| _version_ | 1818304028866510848 |
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| author | Morten Enersen |
| author_facet | Morten Enersen |
| author_sort | Morten Enersen |
| collection | DOAJ |
| description | The introduction of multilocus sequence typing (MLST) in infectious disease research has allowed standardized typing of bacterial clones. Through multiple markers around the genome, it is possible to determine the sequence type (ST) of bacterial isolates to establish the population structure of a species. For the periodontal pathogen, Porphyromonas gingivalis, the MLST scheme has been established at www.pubmlst.org/pgingivalis, and data from the database indicate a high degree of genetic diversity and a weakly clonal population structure comparable with Neisseria menigitidis. The major fimbriae (FimA) have been held responsible for the adhesive properties of P. gingivalis and represent an important virulence factor. The fimA genotyping method (PCR based) indicate that fimA genotype II, IV and Ib are associated with diseased sites in periodontitis and tissue specimens from cardiovascular disease. fimA genotyping of the isolates in the MLST database supports the association of genotypes II and IV with periodontitis. As a result of multiple positive PCR reactions in the fimA genotyping, sequencing of the fimA gene revealed only minor nucleotide variation between isolates of the same and different genotypes, suggesting that the method should be redesigned or re-evaluated. Results from several investigations indicate a higher intraindividual heterogeneity of P. gingivalis than found earlier. Detection of multiple STs from one site in several patients with “refractory” periodontitis, showed allelic variation in two housekeeping genes indicating recombination between different clones within the periodontal pocket. |
| first_indexed | 2024-12-13T06:04:11Z |
| format | Article |
| id | doaj.art-336db716429c4440a4de7ef8217544a0 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-13T06:04:11Z |
| publishDate | 2011-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-336db716429c4440a4de7ef8217544a02022-12-21T23:57:16ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972011-11-013011110.3402/jom.v3i0.8487Porphyromonas gingivalis: a clonal pathogen?Morten EnersenThe introduction of multilocus sequence typing (MLST) in infectious disease research has allowed standardized typing of bacterial clones. Through multiple markers around the genome, it is possible to determine the sequence type (ST) of bacterial isolates to establish the population structure of a species. For the periodontal pathogen, Porphyromonas gingivalis, the MLST scheme has been established at www.pubmlst.org/pgingivalis, and data from the database indicate a high degree of genetic diversity and a weakly clonal population structure comparable with Neisseria menigitidis. The major fimbriae (FimA) have been held responsible for the adhesive properties of P. gingivalis and represent an important virulence factor. The fimA genotyping method (PCR based) indicate that fimA genotype II, IV and Ib are associated with diseased sites in periodontitis and tissue specimens from cardiovascular disease. fimA genotyping of the isolates in the MLST database supports the association of genotypes II and IV with periodontitis. As a result of multiple positive PCR reactions in the fimA genotyping, sequencing of the fimA gene revealed only minor nucleotide variation between isolates of the same and different genotypes, suggesting that the method should be redesigned or re-evaluated. Results from several investigations indicate a higher intraindividual heterogeneity of P. gingivalis than found earlier. Detection of multiple STs from one site in several patients with “refractory” periodontitis, showed allelic variation in two housekeeping genes indicating recombination between different clones within the periodontal pocket.http://www.journaloforalmicrobiology.net/index.php/jom/article/view/8487/16563periodontitisPorphyromonas gingivalisMLSTpopulation geneticsrecombinationfimA genotyping |
| spellingShingle | Morten Enersen Porphyromonas gingivalis: a clonal pathogen? Journal of Oral Microbiology periodontitis Porphyromonas gingivalis MLST population genetics recombination fimA genotyping |
| title | Porphyromonas gingivalis: a clonal pathogen? |
| title_full | Porphyromonas gingivalis: a clonal pathogen? |
| title_fullStr | Porphyromonas gingivalis: a clonal pathogen? |
| title_full_unstemmed | Porphyromonas gingivalis: a clonal pathogen? |
| title_short | Porphyromonas gingivalis: a clonal pathogen? |
| title_sort | porphyromonas gingivalis a clonal pathogen |
| topic | periodontitis Porphyromonas gingivalis MLST population genetics recombination fimA genotyping |
| url | http://www.journaloforalmicrobiology.net/index.php/jom/article/view/8487/16563 |
| work_keys_str_mv | AT mortenenersen porphyromonasgingivalisaclonalpathogen |