Using a Hybrid Mapping Population to Identify Genomic Regions of Pyrenophora teres Associated With Virulence
Net blotches caused by Pyrenophora teres are important foliar fungal diseases of barley and result in significant yield losses of up to 40%. The two types of net blotch, net-form net blotch and spot-form net blotch, are caused by P. teres f. teres (Ptt) and P. teres f. maculata (Ptm), respectively....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.925107/full |
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| author | Buddhika A. Dahanayaka Lislé Snyman Niloofar Vaghefi Niloofar Vaghefi Anke Martin |
| author_facet | Buddhika A. Dahanayaka Lislé Snyman Niloofar Vaghefi Niloofar Vaghefi Anke Martin |
| author_sort | Buddhika A. Dahanayaka |
| collection | DOAJ |
| description | Net blotches caused by Pyrenophora teres are important foliar fungal diseases of barley and result in significant yield losses of up to 40%. The two types of net blotch, net-form net blotch and spot-form net blotch, are caused by P. teres f. teres (Ptt) and P. teres f. maculata (Ptm), respectively. This study is the first to use a cross between Ptt and Ptm to identify quantitative trait loci (QTL) associated with virulence and leaf symptoms. A genetic map consisting of 1,965 Diversity Arrays Technology (DArT) markers was constructed using 351 progenies of the Ptt/Ptm cross. Eight barley cultivars showing differential reactions to the parental isolates were used to phenotype the hybrid progeny isolates. Five QTL associated with virulence and four QTL associated with leaf symptoms were identified across five linkage groups. Phenotypic variation explained by these QTL ranged from 6 to 16%. Further phenotyping of selected progeny isolates on 12 more barley cultivars revealed that three progeny isolates are moderately to highly virulent across these cultivars. The results of this study suggest that accumulation of QTL in hybrid isolates can result in enhanced virulence. |
| first_indexed | 2024-04-13T19:34:16Z |
| format | Article |
| id | doaj.art-336ff22bc2714de4b4c07fa822e571b7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T19:34:16Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-336ff22bc2714de4b4c07fa822e571b72022-12-22T02:33:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.925107925107Using a Hybrid Mapping Population to Identify Genomic Regions of Pyrenophora teres Associated With VirulenceBuddhika A. Dahanayaka0Lislé Snyman1Niloofar Vaghefi2Niloofar Vaghefi3Anke Martin4Centre for Crop Health, University of Southern Queensland, Toowoomba, QLD, AustraliaDepartment of Agriculture and Fisheries Queensland, Hermitage Research Facility, Warwick, QLD, AustraliaCentre for Crop Health, University of Southern Queensland, Toowoomba, QLD, AustraliaSchool of Agriculture and Food, University of Melbourne, Parkville, VIC, AustraliaCentre for Crop Health, University of Southern Queensland, Toowoomba, QLD, AustraliaNet blotches caused by Pyrenophora teres are important foliar fungal diseases of barley and result in significant yield losses of up to 40%. The two types of net blotch, net-form net blotch and spot-form net blotch, are caused by P. teres f. teres (Ptt) and P. teres f. maculata (Ptm), respectively. This study is the first to use a cross between Ptt and Ptm to identify quantitative trait loci (QTL) associated with virulence and leaf symptoms. A genetic map consisting of 1,965 Diversity Arrays Technology (DArT) markers was constructed using 351 progenies of the Ptt/Ptm cross. Eight barley cultivars showing differential reactions to the parental isolates were used to phenotype the hybrid progeny isolates. Five QTL associated with virulence and four QTL associated with leaf symptoms were identified across five linkage groups. Phenotypic variation explained by these QTL ranged from 6 to 16%. Further phenotyping of selected progeny isolates on 12 more barley cultivars revealed that three progeny isolates are moderately to highly virulent across these cultivars. The results of this study suggest that accumulation of QTL in hybrid isolates can result in enhanced virulence.https://www.frontiersin.org/articles/10.3389/fpls.2022.925107/fullhybridsquantitative trait locibarleycandidate genesleaf symptomsnet-form net blotch |
| spellingShingle | Buddhika A. Dahanayaka Lislé Snyman Niloofar Vaghefi Niloofar Vaghefi Anke Martin Using a Hybrid Mapping Population to Identify Genomic Regions of Pyrenophora teres Associated With Virulence Frontiers in Plant Science hybrids quantitative trait loci barley candidate genes leaf symptoms net-form net blotch |
| title | Using a Hybrid Mapping Population to Identify Genomic Regions of Pyrenophora teres Associated With Virulence |
| title_full | Using a Hybrid Mapping Population to Identify Genomic Regions of Pyrenophora teres Associated With Virulence |
| title_fullStr | Using a Hybrid Mapping Population to Identify Genomic Regions of Pyrenophora teres Associated With Virulence |
| title_full_unstemmed | Using a Hybrid Mapping Population to Identify Genomic Regions of Pyrenophora teres Associated With Virulence |
| title_short | Using a Hybrid Mapping Population to Identify Genomic Regions of Pyrenophora teres Associated With Virulence |
| title_sort | using a hybrid mapping population to identify genomic regions of pyrenophora teres associated with virulence |
| topic | hybrids quantitative trait loci barley candidate genes leaf symptoms net-form net blotch |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.925107/full |
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