Effect of LncRNA XIST on Immune Cells of Primary Biliary Cholangitis
ObjectivePrimary biliary cholangitis (PBC) is an autoimmune disease with significant gender difference. X chromosome inactivation (XCI) plays important roles in susceptibility to diseases between genders. This work focuses on the differences of LncRNA XIST in several defined immune cells populations...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.816433/full |
| _version_ | 1828544069712740352 |
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| author | Chunhui She Yifei Yang Bo Zang Yuan Yao Qixuan Liu Patrick S. C. Leung Bin Liu |
| author_facet | Chunhui She Yifei Yang Bo Zang Yuan Yao Qixuan Liu Patrick S. C. Leung Bin Liu |
| author_sort | Chunhui She |
| collection | DOAJ |
| description | ObjectivePrimary biliary cholangitis (PBC) is an autoimmune disease with significant gender difference. X chromosome inactivation (XCI) plays important roles in susceptibility to diseases between genders. This work focuses on the differences of LncRNA XIST in several defined immune cells populations as well as its effects on naive CD4+ T cells proliferation and differentiation in patients with PBC.MethodsNKs, B cells, CD4+ T, and CD8+ T cells were separated by MicroBeads from peripheral blood mononuclear cells (PBMCs) of PBC patients and healthy control (HC). The expression levels of LncRNA XIST in these immune cells were quantified by qRT-PCR and their subcellular localized analyzed by FISH. Lentivirus were used to interfere the expression of LncRNA XIST, and CCK8 was used to detect the proliferation of naive CD4+ T cells in PBC patients. Finally, naive CD4+ T cells were co-cultured with the bile duct epithelial cells (BECs), and the effects of LncRNA XIST on the typing of naive CD4+ T cells and related cytokines were determined by qRT-PCR and ELISA.ResultsThe expression levels of LncRNA XIST in NKs and CD4+ T cells in PBC patients were significantly higher than those in HC, and were primarily located at the nucleus. LncRNA XIST could promote the proliferation of naive CD4+ T cells. When naive CD4+ T cells were co-cultured with BECs, the expressions of IFN-γ, IL-17, T-bet and RORγt in naive CD4+ T cells were decreased.ConclusionLncRNA XIST was associated with lymphocyte abnormalities in patients with PBC. The high expression of LncRNA XIST could stimulate proliferation and differentiation of naive CD4+ T cells, which might account for the high occurrence of PBC in female. |
| first_indexed | 2024-12-12T02:24:47Z |
| format | Article |
| id | doaj.art-33703a394427423e9cfca5a2185c468a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T02:24:47Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-33703a394427423e9cfca5a2185c468a2022-12-22T00:41:35ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-03-011310.3389/fimmu.2022.816433816433Effect of LncRNA XIST on Immune Cells of Primary Biliary CholangitisChunhui She0Yifei Yang1Bo Zang2Yuan Yao3Qixuan Liu4Patrick S. C. Leung5Bin Liu6Department of Rheumatology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Rheumatology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Rheumatology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Rheumatology, Affiliated Hospital of Qingdao University, Qingdao, ChinaEpidemiology and Biostatistics, Maternal and Child Health, School of Public Health (SPH) Department, Boston University, Boston, MA, United StatesDivision of Rheumatology, Allergy and Clinical Immunology, University of California at Davis, Davis, CA, United StatesDepartment of Rheumatology, Affiliated Hospital of Qingdao University, Qingdao, ChinaObjectivePrimary biliary cholangitis (PBC) is an autoimmune disease with significant gender difference. X chromosome inactivation (XCI) plays important roles in susceptibility to diseases between genders. This work focuses on the differences of LncRNA XIST in several defined immune cells populations as well as its effects on naive CD4+ T cells proliferation and differentiation in patients with PBC.MethodsNKs, B cells, CD4+ T, and CD8+ T cells were separated by MicroBeads from peripheral blood mononuclear cells (PBMCs) of PBC patients and healthy control (HC). The expression levels of LncRNA XIST in these immune cells were quantified by qRT-PCR and their subcellular localized analyzed by FISH. Lentivirus were used to interfere the expression of LncRNA XIST, and CCK8 was used to detect the proliferation of naive CD4+ T cells in PBC patients. Finally, naive CD4+ T cells were co-cultured with the bile duct epithelial cells (BECs), and the effects of LncRNA XIST on the typing of naive CD4+ T cells and related cytokines were determined by qRT-PCR and ELISA.ResultsThe expression levels of LncRNA XIST in NKs and CD4+ T cells in PBC patients were significantly higher than those in HC, and were primarily located at the nucleus. LncRNA XIST could promote the proliferation of naive CD4+ T cells. When naive CD4+ T cells were co-cultured with BECs, the expressions of IFN-γ, IL-17, T-bet and RORγt in naive CD4+ T cells were decreased.ConclusionLncRNA XIST was associated with lymphocyte abnormalities in patients with PBC. The high expression of LncRNA XIST could stimulate proliferation and differentiation of naive CD4+ T cells, which might account for the high occurrence of PBC in female.https://www.frontiersin.org/articles/10.3389/fimmu.2022.816433/fullPrimary biliary cholangitisLncRNA XISTCD4+ T cellsNKsTh1Th17 |
| spellingShingle | Chunhui She Yifei Yang Bo Zang Yuan Yao Qixuan Liu Patrick S. C. Leung Bin Liu Effect of LncRNA XIST on Immune Cells of Primary Biliary Cholangitis Frontiers in Immunology Primary biliary cholangitis LncRNA XIST CD4+ T cells NKs Th1 Th17 |
| title | Effect of LncRNA XIST on Immune Cells of Primary Biliary Cholangitis |
| title_full | Effect of LncRNA XIST on Immune Cells of Primary Biliary Cholangitis |
| title_fullStr | Effect of LncRNA XIST on Immune Cells of Primary Biliary Cholangitis |
| title_full_unstemmed | Effect of LncRNA XIST on Immune Cells of Primary Biliary Cholangitis |
| title_short | Effect of LncRNA XIST on Immune Cells of Primary Biliary Cholangitis |
| title_sort | effect of lncrna xist on immune cells of primary biliary cholangitis |
| topic | Primary biliary cholangitis LncRNA XIST CD4+ T cells NKs Th1 Th17 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.816433/full |
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