Thin-layer chromatography of ceramides

Ceramides with mono-, di-, and trihydroxy long-chain bases, and normal (saturated and unsaturated), branched-chain, and 2-hydroxy fatty acids have been analyzed by thin-layer chromatography. In most cases the compounds were also run as acetates. Borate, arsenite, and silver ions were used as complex...

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Main Authors: KARL-ANDERS KARLSSON, IRMIN PASCHER
Format: Article
Language:English
Published: Elsevier 1971-07-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520394967
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author KARL-ANDERS KARLSSON
IRMIN PASCHER
author_facet KARL-ANDERS KARLSSON
IRMIN PASCHER
author_sort KARL-ANDERS KARLSSON
collection DOAJ
description Ceramides with mono-, di-, and trihydroxy long-chain bases, and normal (saturated and unsaturated), branched-chain, and 2-hydroxy fatty acids have been analyzed by thin-layer chromatography. In most cases the compounds were also run as acetates. Borate, arsenite, and silver ions were used as complexing agents, and the effects of number, position, and stereochemistry of hydroxy groups, and of unsaturation, were studied. The results are discussed in view of analysis of natural ceramide species.
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spelling doaj.art-3371541a2a48498c8e6fb0829ed856562022-12-21T17:17:10ZengElsevierJournal of Lipid Research0022-22751971-07-01124466472Thin-layer chromatography of ceramidesKARL-ANDERS KARLSSON0IRMIN PASCHER1Department of Medical Biochemistry, University of Göteborg, Fack, 400 33 Göteborg 33, SwedenDepartment of Medical Biochemistry, University of Göteborg, Fack, 400 33 Göteborg 33, SwedenCeramides with mono-, di-, and trihydroxy long-chain bases, and normal (saturated and unsaturated), branched-chain, and 2-hydroxy fatty acids have been analyzed by thin-layer chromatography. In most cases the compounds were also run as acetates. Borate, arsenite, and silver ions were used as complexing agents, and the effects of number, position, and stereochemistry of hydroxy groups, and of unsaturation, were studied. The results are discussed in view of analysis of natural ceramide species.http://www.sciencedirect.com/science/article/pii/S0022227520394967sphingosinephytosphingosinedienic long-chain basenormal fatty acids2-hydroxy fatty acidsacetyl derivatives
spellingShingle KARL-ANDERS KARLSSON
IRMIN PASCHER
Thin-layer chromatography of ceramides
Journal of Lipid Research
sphingosine
phytosphingosine
dienic long-chain base
normal fatty acids
2-hydroxy fatty acids
acetyl derivatives
title Thin-layer chromatography of ceramides
title_full Thin-layer chromatography of ceramides
title_fullStr Thin-layer chromatography of ceramides
title_full_unstemmed Thin-layer chromatography of ceramides
title_short Thin-layer chromatography of ceramides
title_sort thin layer chromatography of ceramides
topic sphingosine
phytosphingosine
dienic long-chain base
normal fatty acids
2-hydroxy fatty acids
acetyl derivatives
url http://www.sciencedirect.com/science/article/pii/S0022227520394967
work_keys_str_mv AT karlanderskarlsson thinlayerchromatographyofceramides
AT irminpascher thinlayerchromatographyofceramides