<i>Botrytis cinerea</i> Transcriptome during the Infection Process of the Bryophyte <i>Physcomitrium patens</i> and Angiosperms
<i>Botrytis cinerea</i> is a necrotrophic pathogen that causes grey mold in many plant species, including crops and model plants of angiosperms. <i>B. cinerea</i> also infects and colonizes the bryophyte <i>Physcomitrium patens</i> (previously <i>Physcomitre...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/7/1/11 |
| _version_ | 1797543286030729216 |
|---|---|
| author | Guillermo Reboledo Astrid Agorio Lucía Vignale Ramón Alberto Batista-García Inés Ponce De León |
| author_facet | Guillermo Reboledo Astrid Agorio Lucía Vignale Ramón Alberto Batista-García Inés Ponce De León |
| author_sort | Guillermo Reboledo |
| collection | DOAJ |
| description | <i>Botrytis cinerea</i> is a necrotrophic pathogen that causes grey mold in many plant species, including crops and model plants of angiosperms. <i>B. cinerea</i> also infects and colonizes the bryophyte <i>Physcomitrium patens</i> (previously <i>Physcomitrella patens</i>), which perceives the pathogen and activates defense mechanisms. However, these defenses are not sufficient to stop fungal invasion, leading finally to plant decay. To gain more insights into <i>B. cinerea</i> infection and virulence strategies displayed during moss colonization, we performed genome wide transcriptional profiling of <i>B. cinerea</i> during different infection stages. We show that, in total, 1015 <i>B. cinerea</i> genes were differentially expressed in moss tissues. Expression patterns of upregulated genes and gene ontology enrichment analysis revealed that infection of <i>P. patens</i> tissues by <i>B. cinerea</i> depends on reactive oxygen species generation and detoxification, transporter activities, plant cell wall degradation and modification, toxin production and probable plant defense evasion by effector proteins. Moreover, a comparison with available RNAseq data during angiosperm infection, including <i>Arabidopsis thaliana, Solanum lycopersicum and Lactuca sativa</i>, suggests that <i>B. cinerea</i> has virulence and infection functions used in all hosts, while others are more specific to <i>P. patens</i> or angiosperms. |
| first_indexed | 2024-03-10T13:43:23Z |
| format | Article |
| id | doaj.art-3374fc6e67584651a07bee4f8f36af7d |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T13:43:23Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-3374fc6e67584651a07bee4f8f36af7d2023-11-21T02:48:35ZengMDPI AGJournal of Fungi2309-608X2020-12-01711110.3390/jof7010011<i>Botrytis cinerea</i> Transcriptome during the Infection Process of the Bryophyte <i>Physcomitrium patens</i> and AngiospermsGuillermo Reboledo0Astrid Agorio1Lucía Vignale2Ramón Alberto Batista-García3Inés Ponce De León4Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, UruguayDepartamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, UruguayDepartamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, UruguayCentro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, MexicoDepartamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, Uruguay<i>Botrytis cinerea</i> is a necrotrophic pathogen that causes grey mold in many plant species, including crops and model plants of angiosperms. <i>B. cinerea</i> also infects and colonizes the bryophyte <i>Physcomitrium patens</i> (previously <i>Physcomitrella patens</i>), which perceives the pathogen and activates defense mechanisms. However, these defenses are not sufficient to stop fungal invasion, leading finally to plant decay. To gain more insights into <i>B. cinerea</i> infection and virulence strategies displayed during moss colonization, we performed genome wide transcriptional profiling of <i>B. cinerea</i> during different infection stages. We show that, in total, 1015 <i>B. cinerea</i> genes were differentially expressed in moss tissues. Expression patterns of upregulated genes and gene ontology enrichment analysis revealed that infection of <i>P. patens</i> tissues by <i>B. cinerea</i> depends on reactive oxygen species generation and detoxification, transporter activities, plant cell wall degradation and modification, toxin production and probable plant defense evasion by effector proteins. Moreover, a comparison with available RNAseq data during angiosperm infection, including <i>Arabidopsis thaliana, Solanum lycopersicum and Lactuca sativa</i>, suggests that <i>B. cinerea</i> has virulence and infection functions used in all hosts, while others are more specific to <i>P. patens</i> or angiosperms.https://www.mdpi.com/2309-608X/7/1/11<i>Botrytis cinerea</i>transcriptome<i>P. patens</i>angiospermsROScell wall degrading enzymes |
| spellingShingle | Guillermo Reboledo Astrid Agorio Lucía Vignale Ramón Alberto Batista-García Inés Ponce De León <i>Botrytis cinerea</i> Transcriptome during the Infection Process of the Bryophyte <i>Physcomitrium patens</i> and Angiosperms Journal of Fungi <i>Botrytis cinerea</i> transcriptome <i>P. patens</i> angiosperms ROS cell wall degrading enzymes |
| title | <i>Botrytis cinerea</i> Transcriptome during the Infection Process of the Bryophyte <i>Physcomitrium patens</i> and Angiosperms |
| title_full | <i>Botrytis cinerea</i> Transcriptome during the Infection Process of the Bryophyte <i>Physcomitrium patens</i> and Angiosperms |
| title_fullStr | <i>Botrytis cinerea</i> Transcriptome during the Infection Process of the Bryophyte <i>Physcomitrium patens</i> and Angiosperms |
| title_full_unstemmed | <i>Botrytis cinerea</i> Transcriptome during the Infection Process of the Bryophyte <i>Physcomitrium patens</i> and Angiosperms |
| title_short | <i>Botrytis cinerea</i> Transcriptome during the Infection Process of the Bryophyte <i>Physcomitrium patens</i> and Angiosperms |
| title_sort | i botrytis cinerea i transcriptome during the infection process of the bryophyte i physcomitrium patens i and angiosperms |
| topic | <i>Botrytis cinerea</i> transcriptome <i>P. patens</i> angiosperms ROS cell wall degrading enzymes |
| url | https://www.mdpi.com/2309-608X/7/1/11 |
| work_keys_str_mv | AT guillermoreboledo ibotrytiscinereaitranscriptomeduringtheinfectionprocessofthebryophyteiphyscomitriumpatensiandangiosperms AT astridagorio ibotrytiscinereaitranscriptomeduringtheinfectionprocessofthebryophyteiphyscomitriumpatensiandangiosperms AT luciavignale ibotrytiscinereaitranscriptomeduringtheinfectionprocessofthebryophyteiphyscomitriumpatensiandangiosperms AT ramonalbertobatistagarcia ibotrytiscinereaitranscriptomeduringtheinfectionprocessofthebryophyteiphyscomitriumpatensiandangiosperms AT inesponcedeleon ibotrytiscinereaitranscriptomeduringtheinfectionprocessofthebryophyteiphyscomitriumpatensiandangiosperms |