Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain
An expression plasmid (pCFA-1) carrying the cfaB gene that codes for the enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin colonization factor antigen I (CFA/I) subunit was constructed and used to transform a derivative of the attenuated Salmonella typhimurium aroA vaccine strain SL3261 carry...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1998-04-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400012 |
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| author | H.C.R. Guillobel M.G. Luna E.F. Camacho D.F. Almeida L.C.S. Ferreira |
| author_facet | H.C.R. Guillobel M.G. Luna E.F. Camacho D.F. Almeida L.C.S. Ferreira |
| author_sort | H.C.R. Guillobel |
| collection | DOAJ |
| description | An expression plasmid (pCFA-1) carrying the cfaB gene that codes for the enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin colonization factor antigen I (CFA/I) subunit was constructed and used to transform a derivative of the attenuated Salmonella typhimurium aroA vaccine strain SL3261 carrying an F'lacIq. Treatment of the transformed strain with isopropyl-ß-D-thiogalactopyranoside (IPTG) resulted in elevated in vitro expression of the CFA/I subunit. Although flagellar function and lipopolysaccharide (LPS) synthesis were similar in both the parental and the recombinant strains, spleen colonization was reduced in the recombinant strain. All BALB/c mice parenterally inoculated with the recombinant strain developed significant anti-CFA/I and anti-LPS serum antibody titers (P<0.05). Moreover, 2 of 5 mice orally inoculated with the engineered Salmonella strain developed anti-CFA/I intestinal IgA (P>0.05) while 4/5 of the same mice developed anti-LPS IgA (P<0.05). The results indicate that the vaccine strain elicited an antibody response against the bacterial host both after oral and intravenous immunization while the response against the CFA/I antigen was significant only after inoculation by the intravenous route |
| first_indexed | 2024-12-10T16:02:51Z |
| format | Article |
| id | doaj.art-3376aa350f164f668bf20f2ebbf169a7 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-10T16:02:51Z |
| publishDate | 1998-04-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-3376aa350f164f668bf20f2ebbf169a72022-12-22T01:42:20ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1998-04-0131454510.1590/S0100-879X1998000400012Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strainH.C.R. GuillobelM.G. LunaE.F. CamachoD.F. AlmeidaL.C.S. FerreiraAn expression plasmid (pCFA-1) carrying the cfaB gene that codes for the enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin colonization factor antigen I (CFA/I) subunit was constructed and used to transform a derivative of the attenuated Salmonella typhimurium aroA vaccine strain SL3261 carrying an F'lacIq. Treatment of the transformed strain with isopropyl-ß-D-thiogalactopyranoside (IPTG) resulted in elevated in vitro expression of the CFA/I subunit. Although flagellar function and lipopolysaccharide (LPS) synthesis were similar in both the parental and the recombinant strains, spleen colonization was reduced in the recombinant strain. All BALB/c mice parenterally inoculated with the recombinant strain developed significant anti-CFA/I and anti-LPS serum antibody titers (P<0.05). Moreover, 2 of 5 mice orally inoculated with the engineered Salmonella strain developed anti-CFA/I intestinal IgA (P>0.05) while 4/5 of the same mice developed anti-LPS IgA (P<0.05). The results indicate that the vaccine strain elicited an antibody response against the bacterial host both after oral and intravenous immunization while the response against the CFA/I antigen was significant only after inoculation by the intravenous routehttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400012Salmonella typhimurium aroAenterotoxigenic Escherichia coliattenuated vaccinescolonization factor antigen I |
| spellingShingle | H.C.R. Guillobel M.G. Luna E.F. Camacho D.F. Almeida L.C.S. Ferreira Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain Brazilian Journal of Medical and Biological Research Salmonella typhimurium aroA enterotoxigenic Escherichia coli attenuated vaccines colonization factor antigen I |
| title | Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain |
| title_full | Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain |
| title_fullStr | Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain |
| title_full_unstemmed | Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain |
| title_short | Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain |
| title_sort | immunization against the colonization factor antigen i of enterotoxigenic escherichia coli by administration of a bivalent salmonella typhimurium aroa strain |
| topic | Salmonella typhimurium aroA enterotoxigenic Escherichia coli attenuated vaccines colonization factor antigen I |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400012 |
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