Detection of the clarithromycin resistance of Helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real‐time PCR
Abstract The goal of this study was to evaluate the feasibility of detecting Helicobacter pylori clarithromycin resistance in gastric mucosa using the amplification refractory mutation system combined with quantitative real‐time PCR (ARMS‐PCR). Gastric mucosal specimens (150) were collected from pat...
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| Format: | Article |
| Language: | English |
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Wiley
2019-04-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.1986 |
| _version_ | 1797387174408093696 |
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| author | Xiao‐Yan Zhang Wei‐Xiang Shen Chun‐Feng Chen Hai‐Hui Sheng Hong Cheng Jiang Li Fulian Hu Da‐Ru Lu Heng‐Jun Gao |
| author_facet | Xiao‐Yan Zhang Wei‐Xiang Shen Chun‐Feng Chen Hai‐Hui Sheng Hong Cheng Jiang Li Fulian Hu Da‐Ru Lu Heng‐Jun Gao |
| author_sort | Xiao‐Yan Zhang |
| collection | DOAJ |
| description | Abstract The goal of this study was to evaluate the feasibility of detecting Helicobacter pylori clarithromycin resistance in gastric mucosa using the amplification refractory mutation system combined with quantitative real‐time PCR (ARMS‐PCR). Gastric mucosal specimens (150) were collected from patients who were unsuccessfully treated for H. pylori eradication. Each specimen was divided into 2 samples. One sample was used to extract genomic DNA and detect any gene mutations of H. pylori produced by ARMS‐ PCR. Sequencing was used to assess the accuracy of this method. The other sample was used to culture H. pylori. The E‐test minimum inhibitory concentration (MIC) was used to assess clarithromycin resistance. The results were compared with a paired chi‐square test to validate the coincidence rate among the 3 methods. The coincidence rate between the sequencing and ARMS‐PCR results was 98.7%, thus verifying the accuracy of ARMS‐PCR. E‐tests detected 144 clarithromycin resistance cases, including 45 sensitivity cases; the resistance rate was 70%. The coincidence rate between the results of the E‐test and ARMS‐PCR was 97.1%, and no significant difference between the 2 methods was observed. ARMS‐PCR is a simple and fast method that has high sensitivity and specificity and can be used to detect the clarithromycin resistance of H. pylori in gastric mucosa. ARMS‐PCR is expected to be used to study drug resistance mechanisms and use in assays of individual therapies for H. pylori eradication. |
| first_indexed | 2024-03-08T22:20:01Z |
| format | Article |
| id | doaj.art-3376e4b9aee04d5282c7e5fcff43ff85 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-03-08T22:20:01Z |
| publishDate | 2019-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-3376e4b9aee04d5282c7e5fcff43ff852023-12-18T12:55:50ZengWileyCancer Medicine2045-76342019-04-01841633164010.1002/cam4.1986Detection of the clarithromycin resistance of Helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real‐time PCRXiao‐Yan Zhang0Wei‐Xiang Shen1Chun‐Feng Chen2Hai‐Hui Sheng3Hong Cheng4Jiang Li5Fulian Hu6Da‐Ru Lu7Heng‐Jun Gao8State Key Laboratory of Genetic Engineering, School of Life Sciences Fudan University Shanghai ChinaNational Engineering Center for Biochip at Shanghai Shanghai ChinaNational Engineering Center for Biochip at Shanghai Shanghai ChinaNational Engineering Center for Biochip at Shanghai Shanghai ChinaDepartment of Gastroenterology Peking University First Hospital Beijing ChinaDepartment of Gastroenterology Peking University First Hospital Beijing ChinaDepartment of Gastroenterology Peking University First Hospital Beijing ChinaState Key Laboratory of Genetic Engineering, School of Life Sciences Fudan University Shanghai ChinaNational Engineering Center for Biochip at Shanghai Shanghai ChinaAbstract The goal of this study was to evaluate the feasibility of detecting Helicobacter pylori clarithromycin resistance in gastric mucosa using the amplification refractory mutation system combined with quantitative real‐time PCR (ARMS‐PCR). Gastric mucosal specimens (150) were collected from patients who were unsuccessfully treated for H. pylori eradication. Each specimen was divided into 2 samples. One sample was used to extract genomic DNA and detect any gene mutations of H. pylori produced by ARMS‐ PCR. Sequencing was used to assess the accuracy of this method. The other sample was used to culture H. pylori. The E‐test minimum inhibitory concentration (MIC) was used to assess clarithromycin resistance. The results were compared with a paired chi‐square test to validate the coincidence rate among the 3 methods. The coincidence rate between the sequencing and ARMS‐PCR results was 98.7%, thus verifying the accuracy of ARMS‐PCR. E‐tests detected 144 clarithromycin resistance cases, including 45 sensitivity cases; the resistance rate was 70%. The coincidence rate between the results of the E‐test and ARMS‐PCR was 97.1%, and no significant difference between the 2 methods was observed. ARMS‐PCR is a simple and fast method that has high sensitivity and specificity and can be used to detect the clarithromycin resistance of H. pylori in gastric mucosa. ARMS‐PCR is expected to be used to study drug resistance mechanisms and use in assays of individual therapies for H. pylori eradication.https://doi.org/10.1002/cam4.1986ARMS‐PCRHelicobacter pylorimutationresistance |
| spellingShingle | Xiao‐Yan Zhang Wei‐Xiang Shen Chun‐Feng Chen Hai‐Hui Sheng Hong Cheng Jiang Li Fulian Hu Da‐Ru Lu Heng‐Jun Gao Detection of the clarithromycin resistance of Helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real‐time PCR Cancer Medicine ARMS‐PCR Helicobacter pylori mutation resistance |
| title | Detection of the clarithromycin resistance of Helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real‐time PCR |
| title_full | Detection of the clarithromycin resistance of Helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real‐time PCR |
| title_fullStr | Detection of the clarithromycin resistance of Helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real‐time PCR |
| title_full_unstemmed | Detection of the clarithromycin resistance of Helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real‐time PCR |
| title_short | Detection of the clarithromycin resistance of Helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real‐time PCR |
| title_sort | detection of the clarithromycin resistance of helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real time pcr |
| topic | ARMS‐PCR Helicobacter pylori mutation resistance |
| url | https://doi.org/10.1002/cam4.1986 |
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