Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats
Background and aim: The etiopathogenesis of inflammatory bowel disease (IBD) is associated with different factors such as genetic, infectious, immunological, and environmental, including modification of the gut microbiota. IBD′s conventional pharmacological therapeutic approaches have become a chall...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022039950 |
| _version_ | 1811173400986517504 |
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| author | Karina Nascimento da Silva Aline Garnevi Fávero William Ribeiro Caroline Marcantonio Ferreira Patrícia Sartorelli Leonardo Cardili Cristina Stewart Bogsan Joice Naiara Bertaglia Pereira Rita de Cássia Sinigaglia Andréa Cristina de Moraes Malinverni Ana Paula Ribeiro Paiotti Sender Jankiel Miszputen Orlando Ambrogini-Júnior |
| author_facet | Karina Nascimento da Silva Aline Garnevi Fávero William Ribeiro Caroline Marcantonio Ferreira Patrícia Sartorelli Leonardo Cardili Cristina Stewart Bogsan Joice Naiara Bertaglia Pereira Rita de Cássia Sinigaglia Andréa Cristina de Moraes Malinverni Ana Paula Ribeiro Paiotti Sender Jankiel Miszputen Orlando Ambrogini-Júnior |
| author_sort | Karina Nascimento da Silva |
| collection | DOAJ |
| description | Background and aim: The etiopathogenesis of inflammatory bowel disease (IBD) is associated with different factors such as genetic, infectious, immunological, and environmental, including modification of the gut microbiota. IBD′s conventional pharmacological therapeutic approaches have become a challenge due to side effects, complications from prolonged use, and higher costs. Kefir fermented milk beverage is a functional food that has demonstrated multiple beneficial effects including anti-inflammatory and antioxidant activity. Alternative therapeutic strategies have been used for IBD as more natural products with low-cost and easy acquisition. The aim of this study is to evaluate the anti-inflammatory effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats. Methods: We used 4 groups to perform this study: baseline control (BC), kefir control (KC), 5% untreated DSS-induced colitis (DSS), and 5% DSS-induced colitis treated with kefir (DSSK). The animals received fermented kefir milk beverage ad libitum for six days and the disease activity index was recorded daily. Colon samples were processed for Transmission Electron Microscopy and histopathological evaluation. We analyzed short fatty chain acids through the fecal sample using gas chromatography. Results: Kefir supplementation was able to reduce the clinical activity index and inflammatory process evidenced by decreased neutrophil accumulation, decreased reticulum edema, and increased autophagosomes. Also, showed a trend to increase the levels of acetate and propionate. Conclusions: Our results suggest that kefir fermented milk beverage may have an anti-inflammatory effect minimizing the intestinal damage of DSS-induced colitis. |
| first_indexed | 2024-04-10T17:46:12Z |
| format | Article |
| id | doaj.art-3377482019cb479799a150ee8a5ae9bf |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T17:46:12Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-3377482019cb479799a150ee8a5ae9bf2023-02-03T04:58:41ZengElsevierHeliyon2405-84402023-01-0191e12707Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in ratsKarina Nascimento da Silva0Aline Garnevi Fávero1William Ribeiro2Caroline Marcantonio Ferreira3Patrícia Sartorelli4Leonardo Cardili5Cristina Stewart Bogsan6Joice Naiara Bertaglia Pereira7Rita de Cássia Sinigaglia8Andréa Cristina de Moraes Malinverni9Ana Paula Ribeiro Paiotti10Sender Jankiel Miszputen11Orlando Ambrogini-Júnior12Division of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, BrazilDivision of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, BrazilInstitute of Environmental, Chemistry and Pharmaceutical Sciences, Department of Pharmaceutics Sciences - Universidade Federal de São Paulo, Diadema, SP, BrazilInstitute of Environmental, Chemistry and Pharmaceutical Sciences, Department of Pharmaceutics Sciences - Universidade Federal de São Paulo, Diadema, SP, BrazilInstitute of Environmental, Chemistry and Pharmaceutical Sciences, Department of Pharmaceutics Sciences - Universidade Federal de São Paulo, Diadema, SP, BrazilLaboratory of Experimental and Molecular Pathology, Department of Pathology - Universidade Federal de São Paulo, São Paulo, SP, BrazilLaboratory of Fermented Foods of the Faculty of Pharmaceutical Sciences – University of São PauloButantan Institute, São Paulo, SP, BrazilElectron Microscopy Centre – Universidade Federal de São Paulo, São Paulo, SP, BrazilLaboratory of Experimental and Molecular Pathology, Department of Pathology - Universidade Federal de São Paulo, São Paulo, SP, BrazilDivision of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, Brazil; Corresponding author.Division of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, BrazilDivision of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, BrazilBackground and aim: The etiopathogenesis of inflammatory bowel disease (IBD) is associated with different factors such as genetic, infectious, immunological, and environmental, including modification of the gut microbiota. IBD′s conventional pharmacological therapeutic approaches have become a challenge due to side effects, complications from prolonged use, and higher costs. Kefir fermented milk beverage is a functional food that has demonstrated multiple beneficial effects including anti-inflammatory and antioxidant activity. Alternative therapeutic strategies have been used for IBD as more natural products with low-cost and easy acquisition. The aim of this study is to evaluate the anti-inflammatory effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats. Methods: We used 4 groups to perform this study: baseline control (BC), kefir control (KC), 5% untreated DSS-induced colitis (DSS), and 5% DSS-induced colitis treated with kefir (DSSK). The animals received fermented kefir milk beverage ad libitum for six days and the disease activity index was recorded daily. Colon samples were processed for Transmission Electron Microscopy and histopathological evaluation. We analyzed short fatty chain acids through the fecal sample using gas chromatography. Results: Kefir supplementation was able to reduce the clinical activity index and inflammatory process evidenced by decreased neutrophil accumulation, decreased reticulum edema, and increased autophagosomes. Also, showed a trend to increase the levels of acetate and propionate. Conclusions: Our results suggest that kefir fermented milk beverage may have an anti-inflammatory effect minimizing the intestinal damage of DSS-induced colitis.http://www.sciencedirect.com/science/article/pii/S2405844022039950Inflammatory bowel diseaseUlcerative colitisProbioticsKefirShort-chain fatty acids |
| spellingShingle | Karina Nascimento da Silva Aline Garnevi Fávero William Ribeiro Caroline Marcantonio Ferreira Patrícia Sartorelli Leonardo Cardili Cristina Stewart Bogsan Joice Naiara Bertaglia Pereira Rita de Cássia Sinigaglia Andréa Cristina de Moraes Malinverni Ana Paula Ribeiro Paiotti Sender Jankiel Miszputen Orlando Ambrogini-Júnior Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats Heliyon Inflammatory bowel disease Ulcerative colitis Probiotics Kefir Short-chain fatty acids |
| title | Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats |
| title_full | Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats |
| title_fullStr | Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats |
| title_full_unstemmed | Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats |
| title_short | Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats |
| title_sort | effects of kefir fermented milk beverage on sodium dextran sulfate dss induced colitis in rats |
| topic | Inflammatory bowel disease Ulcerative colitis Probiotics Kefir Short-chain fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022039950 |
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