Comparison between various DNA sterilization procedures applied in forensic analysis
Abstract Background The advanced sensitive STR kits applied in forensic DNA typing techniques can cause challenging issues when evidence samples are contaminated with minute quantities of DNA from another source such as forensic analysts or crime scene examiners. Results In this study, laboratory ai...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2022-01-01
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| Series: | Egyptian Journal of Forensic Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41935-022-00265-7 |
| _version_ | 1828893091438788608 |
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| author | Noora R. Al-Snan Najib M. Alraimi |
| author_facet | Noora R. Al-Snan Najib M. Alraimi |
| author_sort | Noora R. Al-Snan |
| collection | DOAJ |
| description | Abstract Background The advanced sensitive STR kits applied in forensic DNA typing techniques can cause challenging issues when evidence samples are contaminated with minute quantities of DNA from another source such as forensic analysts or crime scene examiners. Results In this study, laboratory air and surfaces, gloves, tools, and equipment were evaluated as potential sources of contaminating DNA. Different sterilization methods were tested for their ability to efficiently eliminate DNA in a sample. Inactivation methods included 10% bleach, ethanol, UV light, and DNA-ExitusPlus IF. Exposure to the different inactivation protocols for varying periods of time was performed in two lab settings: low template DNA and DNA database labs. Surfaces were swabbed and any adhering DNA was quantified using HID real-time PCR. Results were detected using HID Real-Time PCR Analysis Software v1.2 and GeneMapper ID-X Software v1.4. Conclusions It was concluded that most of the DNA decontamination methods are not suitable for highly sensitive and precision STR kits such as GlobalFiler PCR Amplification Kit. The most suitable tested method was using DNA-ExitusPlus IF with the incubation time increased from 10 to 15 min. |
| first_indexed | 2024-12-13T13:47:34Z |
| format | Article |
| id | doaj.art-3379de11777446a1ba3937e1aa55b0a6 |
| institution | Directory Open Access Journal |
| issn | 2090-5939 |
| language | English |
| last_indexed | 2024-12-13T13:47:34Z |
| publishDate | 2022-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Forensic Sciences |
| spelling | doaj.art-3379de11777446a1ba3937e1aa55b0a62022-12-21T23:43:21ZengSpringerOpenEgyptian Journal of Forensic Sciences2090-59392022-01-0112111110.1186/s41935-022-00265-7Comparison between various DNA sterilization procedures applied in forensic analysisNoora R. Al-Snan0Najib M. Alraimi1Forensic Science Laboratory, Directorate of Forensic Science, General Directorate of Criminal Investigation and Forensic Science, Ministry of InteriorForensic Science Laboratory, Directorate of Forensic Science, General Directorate of Criminal Investigation and Forensic Science, Ministry of InteriorAbstract Background The advanced sensitive STR kits applied in forensic DNA typing techniques can cause challenging issues when evidence samples are contaminated with minute quantities of DNA from another source such as forensic analysts or crime scene examiners. Results In this study, laboratory air and surfaces, gloves, tools, and equipment were evaluated as potential sources of contaminating DNA. Different sterilization methods were tested for their ability to efficiently eliminate DNA in a sample. Inactivation methods included 10% bleach, ethanol, UV light, and DNA-ExitusPlus IF. Exposure to the different inactivation protocols for varying periods of time was performed in two lab settings: low template DNA and DNA database labs. Surfaces were swabbed and any adhering DNA was quantified using HID real-time PCR. Results were detected using HID Real-Time PCR Analysis Software v1.2 and GeneMapper ID-X Software v1.4. Conclusions It was concluded that most of the DNA decontamination methods are not suitable for highly sensitive and precision STR kits such as GlobalFiler PCR Amplification Kit. The most suitable tested method was using DNA-ExitusPlus IF with the incubation time increased from 10 to 15 min.https://doi.org/10.1186/s41935-022-00265-7DNA decontaminationDNA-ExitusPlus IFContaminationSterilizationForensic analysisDNA evidence |
| spellingShingle | Noora R. Al-Snan Najib M. Alraimi Comparison between various DNA sterilization procedures applied in forensic analysis Egyptian Journal of Forensic Sciences DNA decontamination DNA-ExitusPlus IF Contamination Sterilization Forensic analysis DNA evidence |
| title | Comparison between various DNA sterilization procedures applied in forensic analysis |
| title_full | Comparison between various DNA sterilization procedures applied in forensic analysis |
| title_fullStr | Comparison between various DNA sterilization procedures applied in forensic analysis |
| title_full_unstemmed | Comparison between various DNA sterilization procedures applied in forensic analysis |
| title_short | Comparison between various DNA sterilization procedures applied in forensic analysis |
| title_sort | comparison between various dna sterilization procedures applied in forensic analysis |
| topic | DNA decontamination DNA-ExitusPlus IF Contamination Sterilization Forensic analysis DNA evidence |
| url | https://doi.org/10.1186/s41935-022-00265-7 |
| work_keys_str_mv | AT nooraralsnan comparisonbetweenvariousdnasterilizationproceduresappliedinforensicanalysis AT najibmalraimi comparisonbetweenvariousdnasterilizationproceduresappliedinforensicanalysis |