rDNA Condensation Promotes rDNA Separation from Nucleolar Proteins Degraded for Nucleophagy after TORC1 Inactivation
Summary: Nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1) protein kinase induce nucleophagy preferentially degrading only nucleolar components in budding yeast. Nucleolar proteins are relocated to sites proximal to the nucleus-vacuole junction (NVJ), where micronucleopha...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-09-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124719311076 |
| _version_ | 1817997087921405952 |
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| author | Md. Golam Mostofa Shamsul Morshed Ritsu Shibata Yuri Takeichi Muhammad Arifur Rahman Shun Hosoyamada Takehiko Kobayashi Takashi Ushimaru |
| author_facet | Md. Golam Mostofa Shamsul Morshed Ritsu Shibata Yuri Takeichi Muhammad Arifur Rahman Shun Hosoyamada Takehiko Kobayashi Takashi Ushimaru |
| author_sort | Md. Golam Mostofa |
| collection | DOAJ |
| description | Summary: Nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1) protein kinase induce nucleophagy preferentially degrading only nucleolar components in budding yeast. Nucleolar proteins are relocated to sites proximal to the nucleus-vacuole junction (NVJ), where micronucleophagy occurs, whereas rDNA, which is embedded in the nucleolus under normal conditions, moves to NVJ-distal regions, causing rDNA dissociation from nucleolar proteins after TORC1 inactivation. This repositioning is mediated via chromosome linkage INM protein (CLIP)-cohibin complexes that tether rDNA to the inner nuclear membrane. Here, we show that TORC1 inactivation-induced rDNA condensation promotes the repositioning of rDNA and nucleolar proteins. Defects in condensin, Rpd3–Sin3 histone deacetylase (HDAC), and high-mobility group protein 1 (Hmo1), which are involved in TORC1 inactivation-induced rDNA condensation, compromised the repositioning and nucleophagic degradation of nucleolar proteins, although rDNA still escaped from nucleophagic degradation in these mutants. We propose a model in which rDNA condensation after TORC1 inactivation generates a motive force for the repositioning of rDNA and nucleolar proteins. : Nutrient starvation and TORC1 inactivation induce the repositioning of rDNA and nucleolar proteins and nucleophagic degradation of nucleolar proteins in budding yeast. Mostofa et al. report that condensin, Rpd3 HDAC, and Hmo1 promote rDNA condensation, as well as the repositioning and nucleophagic degradation of nucleolar proteins. Keywords: condensin, Hmo1, nucleolus, nucleophagy, rDNA, Rpd3, target of rapamycin complex 1, TORC1 |
| first_indexed | 2024-04-14T02:33:05Z |
| format | Article |
| id | doaj.art-337d05b719de4920b647c5e0498b3e49 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-14T02:33:05Z |
| publishDate | 2019-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-337d05b719de4920b647c5e0498b3e492022-12-22T02:17:36ZengElsevierCell Reports2211-12472019-09-01281334233434.e2rDNA Condensation Promotes rDNA Separation from Nucleolar Proteins Degraded for Nucleophagy after TORC1 InactivationMd. Golam Mostofa0Shamsul Morshed1Ritsu Shibata2Yuri Takeichi3Muhammad Arifur Rahman4Shun Hosoyamada5Takehiko Kobayashi6Takashi Ushimaru7Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka 422-8021, JapanGraduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka 422-8021, JapanDepartment of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka 422-8021, JapanDepartment of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka 422-8021, JapanGraduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka 422-8021, JapanLaboratory of Genome Regeneration, Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, JapanLaboratory of Genome Regeneration, Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, JapanGraduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka 422-8021, Japan; Department of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka 422-8021, Japan; Corresponding authorSummary: Nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1) protein kinase induce nucleophagy preferentially degrading only nucleolar components in budding yeast. Nucleolar proteins are relocated to sites proximal to the nucleus-vacuole junction (NVJ), where micronucleophagy occurs, whereas rDNA, which is embedded in the nucleolus under normal conditions, moves to NVJ-distal regions, causing rDNA dissociation from nucleolar proteins after TORC1 inactivation. This repositioning is mediated via chromosome linkage INM protein (CLIP)-cohibin complexes that tether rDNA to the inner nuclear membrane. Here, we show that TORC1 inactivation-induced rDNA condensation promotes the repositioning of rDNA and nucleolar proteins. Defects in condensin, Rpd3–Sin3 histone deacetylase (HDAC), and high-mobility group protein 1 (Hmo1), which are involved in TORC1 inactivation-induced rDNA condensation, compromised the repositioning and nucleophagic degradation of nucleolar proteins, although rDNA still escaped from nucleophagic degradation in these mutants. We propose a model in which rDNA condensation after TORC1 inactivation generates a motive force for the repositioning of rDNA and nucleolar proteins. : Nutrient starvation and TORC1 inactivation induce the repositioning of rDNA and nucleolar proteins and nucleophagic degradation of nucleolar proteins in budding yeast. Mostofa et al. report that condensin, Rpd3 HDAC, and Hmo1 promote rDNA condensation, as well as the repositioning and nucleophagic degradation of nucleolar proteins. Keywords: condensin, Hmo1, nucleolus, nucleophagy, rDNA, Rpd3, target of rapamycin complex 1, TORC1http://www.sciencedirect.com/science/article/pii/S2211124719311076 |
| spellingShingle | Md. Golam Mostofa Shamsul Morshed Ritsu Shibata Yuri Takeichi Muhammad Arifur Rahman Shun Hosoyamada Takehiko Kobayashi Takashi Ushimaru rDNA Condensation Promotes rDNA Separation from Nucleolar Proteins Degraded for Nucleophagy after TORC1 Inactivation Cell Reports |
| title | rDNA Condensation Promotes rDNA Separation from Nucleolar Proteins Degraded for Nucleophagy after TORC1 Inactivation |
| title_full | rDNA Condensation Promotes rDNA Separation from Nucleolar Proteins Degraded for Nucleophagy after TORC1 Inactivation |
| title_fullStr | rDNA Condensation Promotes rDNA Separation from Nucleolar Proteins Degraded for Nucleophagy after TORC1 Inactivation |
| title_full_unstemmed | rDNA Condensation Promotes rDNA Separation from Nucleolar Proteins Degraded for Nucleophagy after TORC1 Inactivation |
| title_short | rDNA Condensation Promotes rDNA Separation from Nucleolar Proteins Degraded for Nucleophagy after TORC1 Inactivation |
| title_sort | rdna condensation promotes rdna separation from nucleolar proteins degraded for nucleophagy after torc1 inactivation |
| url | http://www.sciencedirect.com/science/article/pii/S2211124719311076 |
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