The Effect of Endostatin and Radiotherapy on Human Lung Cancer Cell Line A549 and the Impact of HIF-1 Expression after Therapy
Background and objective It has been proven that radiotherapy can induce tumor cells over expressing HIF-1. The aim of this work is to investigate the effect of endostatin and radiotherapy on human lung cancer cell line A549 and the impact of HIF-1 expression after therapy. Methods Analysed the cell...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2009-01-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2009.01.005&path%5B%5D=199 |
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| author | Yanyan ZHANG Wei GE Juan ZHAO Zhenyu ZHAO Changhu LI |
| author_facet | Yanyan ZHANG Wei GE Juan ZHAO Zhenyu ZHAO Changhu LI |
| author_sort | Yanyan ZHANG |
| collection | DOAJ |
| description | Background and objective It has been proven that radiotherapy can induce tumor cells over expressing HIF-1. The aim of this work is to investigate the effect of endostatin and radiotherapy on human lung cancer cell line A549 and the impact of HIF-1 expression after therapy. Methods Analysed the cell proliferation periods of theA549 cells after treated by endostatin. The A549 cells were treated with endostatin (ES) and/or radiotherpy (RT), and then the cell growth inhibiting rate was detected by methyl thiazolyl tetrazolium (MTT), the expression of HIF-1 was determined by enzyme linked immunosorbent assay (Elisa). Results Every treatments can inhibit the growth of A549 cells, but the cells treated with endostatin and radiotherapy were inhibited more obviously. Endostatin had the function of block cell periods on A549, it stoped the A549 cells at G2/M and S periods. Treatment of endostatin and radiotherapy can reduce the expression of HIF-1 induced by radiotherapy. Conclusion Endostatin was synergistical with the effect of radiotherapy oninhibiting the growth rate of the A549 cells. This synergism may come from: endostatin can make A549 cells accumulated in G2-M and S transition, thereby inhibiting the proliferation of the A549 cells. HIF-1 is a important factor in resistant of cemotherapy and radiotherapy. Endostatin can inhibite the producing of HIF-1 by cells itself or induced by radiation, so increasing the treatment effect of radiotherapy. |
| first_indexed | 2024-12-11T05:46:34Z |
| format | Article |
| id | doaj.art-337e23df2a2344e09f04c54572c856d8 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-12-11T05:46:34Z |
| publishDate | 2009-01-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-337e23df2a2344e09f04c54572c856d82022-12-22T01:18:57ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872009-01-011213337The Effect of Endostatin and Radiotherapy on Human Lung Cancer Cell Line A549 and the Impact of HIF-1 Expression after TherapyYanyan ZHANGWei GEJuan ZHAOZhenyu ZHAOChanghu LIBackground and objective It has been proven that radiotherapy can induce tumor cells over expressing HIF-1. The aim of this work is to investigate the effect of endostatin and radiotherapy on human lung cancer cell line A549 and the impact of HIF-1 expression after therapy. Methods Analysed the cell proliferation periods of theA549 cells after treated by endostatin. The A549 cells were treated with endostatin (ES) and/or radiotherpy (RT), and then the cell growth inhibiting rate was detected by methyl thiazolyl tetrazolium (MTT), the expression of HIF-1 was determined by enzyme linked immunosorbent assay (Elisa). Results Every treatments can inhibit the growth of A549 cells, but the cells treated with endostatin and radiotherapy were inhibited more obviously. Endostatin had the function of block cell periods on A549, it stoped the A549 cells at G2/M and S periods. Treatment of endostatin and radiotherapy can reduce the expression of HIF-1 induced by radiotherapy. Conclusion Endostatin was synergistical with the effect of radiotherapy oninhibiting the growth rate of the A549 cells. This synergism may come from: endostatin can make A549 cells accumulated in G2-M and S transition, thereby inhibiting the proliferation of the A549 cells. HIF-1 is a important factor in resistant of cemotherapy and radiotherapy. Endostatin can inhibite the producing of HIF-1 by cells itself or induced by radiation, so increasing the treatment effect of radiotherapy.http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2009.01.005&path%5B%5D=199Lung neoplasmsEndostatinRadiotherapyCell cycleHIF-1 |
| spellingShingle | Yanyan ZHANG Wei GE Juan ZHAO Zhenyu ZHAO Changhu LI The Effect of Endostatin and Radiotherapy on Human Lung Cancer Cell Line A549 and the Impact of HIF-1 Expression after Therapy Chinese Journal of Lung Cancer Lung neoplasms Endostatin Radiotherapy Cell cycle HIF-1 |
| title | The Effect of Endostatin and Radiotherapy on Human Lung Cancer Cell Line A549 and the Impact of HIF-1 Expression after Therapy |
| title_full | The Effect of Endostatin and Radiotherapy on Human Lung Cancer Cell Line A549 and the Impact of HIF-1 Expression after Therapy |
| title_fullStr | The Effect of Endostatin and Radiotherapy on Human Lung Cancer Cell Line A549 and the Impact of HIF-1 Expression after Therapy |
| title_full_unstemmed | The Effect of Endostatin and Radiotherapy on Human Lung Cancer Cell Line A549 and the Impact of HIF-1 Expression after Therapy |
| title_short | The Effect of Endostatin and Radiotherapy on Human Lung Cancer Cell Line A549 and the Impact of HIF-1 Expression after Therapy |
| title_sort | effect of endostatin and radiotherapy on human lung cancer cell line a549 and the impact of hif 1 expression after therapy |
| topic | Lung neoplasms Endostatin Radiotherapy Cell cycle HIF-1 |
| url | http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2009.01.005&path%5B%5D=199 |
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