Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cells
Regardless of the known anti-inflammatory potential of heterocyclic antidepressants, the mechanisms concerning their modulating effects are not completely known. In our earlier work, maprotiline, a heterocyclic antidepressants, considerably inhibited infiltration of polymorphonuclear cell leucocytes...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Research in Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.jrps.ir/article.asp?issn=1735-5362;year=2016;volume=11;issue=2;spage=138;epage=144;aulast=Rafiee |
| _version_ | 1818911953496571904 |
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| author | Laleh Rafiee Valiollah Hajhashemi Shaghayegh Haghjooy Javanmard |
| author_facet | Laleh Rafiee Valiollah Hajhashemi Shaghayegh Haghjooy Javanmard |
| author_sort | Laleh Rafiee |
| collection | DOAJ |
| description | Regardless of the known anti-inflammatory potential of heterocyclic antidepressants, the mechanisms concerning their modulating effects are not completely known. In our earlier work, maprotiline, a heterocyclic antidepressants, considerably inhibited infiltration of polymorphonuclear cell leucocytes into the inflamed paw . To understand the mechanism involved, we evaluated the effect of vascular cell adhesion molecule (VCAM-1), intracellular adhesion molecule (ICAM-1) expression in stimulated endothelial cells. Endothelial cells were stimulated with lipopolysaccharide (LPS) in the presence and absence of maprotiline (10 -8 to 10 -6 M) and ICAM-1 and VCAM-1 expression were measured using real-time quantitative reverse transcription polymerase chain reaction. Maprotiline significantly decreased the LPS-induced expression of VCAM-1 at all applied concentrations. The expression of ICAM-1 decreased in the presence of maprotiline at 10 -6 M concentration (P<</i>0.05). Since maprotiline inhibits the expression of adhesion molecules, ICAM-1 and VCAM-1 in LPS-stimulated human endothelial cells , it can be a possible way through which maprotiline exerts its anti-inflammatory properties. |
| first_indexed | 2024-12-19T23:06:53Z |
| format | Article |
| id | doaj.art-337edcb28748428b85bf159aa6a2301a |
| institution | Directory Open Access Journal |
| issn | 1735-5362 1735-9414 |
| language | English |
| last_indexed | 2024-12-19T23:06:53Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj.art-337edcb28748428b85bf159aa6a2301a2022-12-21T20:02:21ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142016-01-01112138144Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cellsLaleh RafieeValiollah HajhashemiShaghayegh Haghjooy JavanmardRegardless of the known anti-inflammatory potential of heterocyclic antidepressants, the mechanisms concerning their modulating effects are not completely known. In our earlier work, maprotiline, a heterocyclic antidepressants, considerably inhibited infiltration of polymorphonuclear cell leucocytes into the inflamed paw . To understand the mechanism involved, we evaluated the effect of vascular cell adhesion molecule (VCAM-1), intracellular adhesion molecule (ICAM-1) expression in stimulated endothelial cells. Endothelial cells were stimulated with lipopolysaccharide (LPS) in the presence and absence of maprotiline (10 -8 to 10 -6 M) and ICAM-1 and VCAM-1 expression were measured using real-time quantitative reverse transcription polymerase chain reaction. Maprotiline significantly decreased the LPS-induced expression of VCAM-1 at all applied concentrations. The expression of ICAM-1 decreased in the presence of maprotiline at 10 -6 M concentration (P<</i>0.05). Since maprotiline inhibits the expression of adhesion molecules, ICAM-1 and VCAM-1 in LPS-stimulated human endothelial cells , it can be a possible way through which maprotiline exerts its anti-inflammatory properties.http://www.jrps.ir/article.asp?issn=1735-5362;year=2016;volume=11;issue=2;spage=138;epage=144;aulast=RafieeMaprotiline; ICAM-1; VCAM-1; Endothelial cells |
| spellingShingle | Laleh Rafiee Valiollah Hajhashemi Shaghayegh Haghjooy Javanmard Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cells Research in Pharmaceutical Sciences Maprotiline; ICAM-1; VCAM-1; Endothelial cells |
| title | Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cells |
| title_full | Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cells |
| title_fullStr | Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cells |
| title_full_unstemmed | Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cells |
| title_short | Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cells |
| title_sort | maprotiline inhibits lps induced expression of adhesion molecules icam 1 and vcam 1 in human endothelial cells |
| topic | Maprotiline; ICAM-1; VCAM-1; Endothelial cells |
| url | http://www.jrps.ir/article.asp?issn=1735-5362;year=2016;volume=11;issue=2;spage=138;epage=144;aulast=Rafiee |
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