CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma
Abstract Background Renal cell carcinoma (RCC) is the most common kidney cancer, accounting for approximately 80–90% of all primary kidney cancer. Treatment for patients with advanced RCC remains unsatisfactory. Rare cancer stem cells (CSCs) are proposed to be responsible for failure of current trea...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12935-017-0473-z |
| _version_ | 1819060003360735232 |
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| author | Du-Chu Chen You-De Liang Liang Peng Yi-Ze Wang Chun-Zhi Ai Xin-Xing Zhu Ya-Wei Yan Yasmeen Saeed Bin Yu Jingying Huang Yuxin Gao Jiaqi Liu Yi-Zhou Jiang Min Liu Demeng Chen |
| author_facet | Du-Chu Chen You-De Liang Liang Peng Yi-Ze Wang Chun-Zhi Ai Xin-Xing Zhu Ya-Wei Yan Yasmeen Saeed Bin Yu Jingying Huang Yuxin Gao Jiaqi Liu Yi-Zhou Jiang Min Liu Demeng Chen |
| author_sort | Du-Chu Chen |
| collection | DOAJ |
| description | Abstract Background Renal cell carcinoma (RCC) is the most common kidney cancer, accounting for approximately 80–90% of all primary kidney cancer. Treatment for patients with advanced RCC remains unsatisfactory. Rare cancer stem cells (CSCs) are proposed to be responsible for failure of current treatment. Methods OncoLnc was used as a tool for interactively exploring survival correlations. Gene manipulation and expression analysis were carried out using siRNA, RT-PCR and Western blotting. Wound healing and invasion assays were used for phenotypical characterization. Aldefluor assay and FACS sorting Sphere culture were used to determine the “stemness” of CSCs. Co-Immunoprecipitation (Co-IP) was used to examine the interaction between OCT4 and CBFA2T2. Student’s t-test and Chi square test was used to analyze statistical significance. Results CBFA2T2 expression can significantly predict the survival of RCC patients. Knocking-down of CBFA2T2 can inhibit cell migration and invasion in RCC cells in vitro, and reduce ALDHhigh CSCs populations. CBFA2T2 expression is necessary for sphere-forming ability and cancer stem cells marker expression in RCC cell lines. Conclusions Our data suggest that CBFA2T2 expression correlates with aggressive characteristics of RCC and CBFA2T2 is required for maintenance of “stemness” through regulation of stem cells factors, thereby highlighting CBFA2T2 as a potential therapeutic target for RCC treatment. |
| first_indexed | 2024-12-21T14:20:05Z |
| format | Article |
| id | doaj.art-33812fac17d545589e9e0983f74a9930 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-21T14:20:05Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-33812fac17d545589e9e0983f74a99302022-12-21T19:00:49ZengBMCCancer Cell International1475-28672017-11-011711810.1186/s12935-017-0473-zCBFA2T2 is associated with a cancer stem cell state in renal cell carcinomaDu-Chu Chen0You-De Liang1Liang Peng2Yi-Ze Wang3Chun-Zhi Ai4Xin-Xing Zhu5Ya-Wei Yan6Yasmeen Saeed7Bin Yu8Jingying Huang9Yuxin Gao10Jiaqi Liu11Yi-Zhou Jiang12Min Liu13Demeng Chen14State Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen UniversityDepartment of Stomatology, Nanshan Affiliated Hospital of Guangdong Medical CollegeDepartment of Oncology, Chinese PLA General HospitalInstitute for Advanced Study, Shenzhen UniversityInstitute for Advanced Study, Shenzhen UniversityInstitute for Advanced Study, Shenzhen UniversityInstitute for Advanced Study, Shenzhen UniversityInstitute for Advanced Study, Shenzhen UniversityState Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen UniversityState Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen UniversityState Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen UniversityState Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen UniversityInstitute for Advanced Study, Shenzhen UniversityState Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen UniversityThe First Affiliated Hospital of Sun Yat-Sen UniversityAbstract Background Renal cell carcinoma (RCC) is the most common kidney cancer, accounting for approximately 80–90% of all primary kidney cancer. Treatment for patients with advanced RCC remains unsatisfactory. Rare cancer stem cells (CSCs) are proposed to be responsible for failure of current treatment. Methods OncoLnc was used as a tool for interactively exploring survival correlations. Gene manipulation and expression analysis were carried out using siRNA, RT-PCR and Western blotting. Wound healing and invasion assays were used for phenotypical characterization. Aldefluor assay and FACS sorting Sphere culture were used to determine the “stemness” of CSCs. Co-Immunoprecipitation (Co-IP) was used to examine the interaction between OCT4 and CBFA2T2. Student’s t-test and Chi square test was used to analyze statistical significance. Results CBFA2T2 expression can significantly predict the survival of RCC patients. Knocking-down of CBFA2T2 can inhibit cell migration and invasion in RCC cells in vitro, and reduce ALDHhigh CSCs populations. CBFA2T2 expression is necessary for sphere-forming ability and cancer stem cells marker expression in RCC cell lines. Conclusions Our data suggest that CBFA2T2 expression correlates with aggressive characteristics of RCC and CBFA2T2 is required for maintenance of “stemness” through regulation of stem cells factors, thereby highlighting CBFA2T2 as a potential therapeutic target for RCC treatment.http://link.springer.com/article/10.1186/s12935-017-0473-zCBFA2T2Renal cell carcinomaCancer stem cellsOCT-4NANOG |
| spellingShingle | Du-Chu Chen You-De Liang Liang Peng Yi-Ze Wang Chun-Zhi Ai Xin-Xing Zhu Ya-Wei Yan Yasmeen Saeed Bin Yu Jingying Huang Yuxin Gao Jiaqi Liu Yi-Zhou Jiang Min Liu Demeng Chen CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma Cancer Cell International CBFA2T2 Renal cell carcinoma Cancer stem cells OCT-4 NANOG |
| title | CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma |
| title_full | CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma |
| title_fullStr | CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma |
| title_full_unstemmed | CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma |
| title_short | CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma |
| title_sort | cbfa2t2 is associated with a cancer stem cell state in renal cell carcinoma |
| topic | CBFA2T2 Renal cell carcinoma Cancer stem cells OCT-4 NANOG |
| url | http://link.springer.com/article/10.1186/s12935-017-0473-z |
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