Polysialic acid modification of the synaptic cell adhesion molecule SynCAM 1 in human embryonic stem cell-derived oligodendrocyte precursor cells
Oligodendrocyte precursor cells (OPCs) are the progenitors of myelinating oligodendrocytes in brain development and repair. Successful myelination depends on the control of adhesiveness during OPC migration and axon contact formation. The decoration of cell surface proteins with the glycan polysiali...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-05-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506115000410 |
| _version_ | 1811192175518547968 |
|---|---|
| author | Sebastian Werneburg Falk F.R. Buettner Martina Mühlenhoff Herbert Hildebrandt |
| author_facet | Sebastian Werneburg Falk F.R. Buettner Martina Mühlenhoff Herbert Hildebrandt |
| author_sort | Sebastian Werneburg |
| collection | DOAJ |
| description | Oligodendrocyte precursor cells (OPCs) are the progenitors of myelinating oligodendrocytes in brain development and repair. Successful myelination depends on the control of adhesiveness during OPC migration and axon contact formation. The decoration of cell surface proteins with the glycan polysialic acid (polySia) is a key regulatory element of OPC interactions during development and under pathological conditions. By far the major protein carrier of polySia is the neural cell adhesion molecule NCAM, but recently, polysialylation of the synaptic cell adhesion molecule SynCAM 1 has been detected in the developing mouse brain. In mice, polySia-SynCAM 1 is associated with cells expressing NG2, a marker of a heterogeneous precursor cell population, which is the primary source for oligodendrocytes in development and myelin repair but can also give rise to astrocytes and possibly neurons. It is not yet clear if polySia-SynCAM 1 is expressed by OPCs and its occurrence in humans is elusive. By generating uniform human embryonic stem cell-derived OPC cultures, we demonstrate that polySia is present on human OPCs but down-regulated during differentiation into myelin basic protein-positive oligodendrocytes. PolySia on NCAM resides on the isoforms NCAM-180 and NCAM-140, and SynCAM 1 is identified as a novel polySia acceptor in human OPCs. |
| first_indexed | 2024-04-11T23:47:26Z |
| format | Article |
| id | doaj.art-3382f1f3cac64472987a855d5ea7b7b9 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 1876-7753 |
| language | English |
| last_indexed | 2024-04-11T23:47:26Z |
| publishDate | 2015-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-3382f1f3cac64472987a855d5ea7b7b92022-12-22T03:56:35ZengElsevierStem Cell Research1873-50611876-77532015-05-0114333934610.1016/j.scr.2015.03.001Polysialic acid modification of the synaptic cell adhesion molecule SynCAM 1 in human embryonic stem cell-derived oligodendrocyte precursor cellsSebastian Werneburg0Falk F.R. Buettner1Martina Mühlenhoff2Herbert Hildebrandt3Institute for Cellular Chemistry, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, GermanyInstitute for Cellular Chemistry, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, GermanyInstitute for Cellular Chemistry, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, GermanyInstitute for Cellular Chemistry, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, GermanyOligodendrocyte precursor cells (OPCs) are the progenitors of myelinating oligodendrocytes in brain development and repair. Successful myelination depends on the control of adhesiveness during OPC migration and axon contact formation. The decoration of cell surface proteins with the glycan polysialic acid (polySia) is a key regulatory element of OPC interactions during development and under pathological conditions. By far the major protein carrier of polySia is the neural cell adhesion molecule NCAM, but recently, polysialylation of the synaptic cell adhesion molecule SynCAM 1 has been detected in the developing mouse brain. In mice, polySia-SynCAM 1 is associated with cells expressing NG2, a marker of a heterogeneous precursor cell population, which is the primary source for oligodendrocytes in development and myelin repair but can also give rise to astrocytes and possibly neurons. It is not yet clear if polySia-SynCAM 1 is expressed by OPCs and its occurrence in humans is elusive. By generating uniform human embryonic stem cell-derived OPC cultures, we demonstrate that polySia is present on human OPCs but down-regulated during differentiation into myelin basic protein-positive oligodendrocytes. PolySia on NCAM resides on the isoforms NCAM-180 and NCAM-140, and SynCAM 1 is identified as a novel polySia acceptor in human OPCs.http://www.sciencedirect.com/science/article/pii/S1873506115000410 |
| spellingShingle | Sebastian Werneburg Falk F.R. Buettner Martina Mühlenhoff Herbert Hildebrandt Polysialic acid modification of the synaptic cell adhesion molecule SynCAM 1 in human embryonic stem cell-derived oligodendrocyte precursor cells Stem Cell Research |
| title | Polysialic acid modification of the synaptic cell adhesion molecule SynCAM 1 in human embryonic stem cell-derived oligodendrocyte precursor cells |
| title_full | Polysialic acid modification of the synaptic cell adhesion molecule SynCAM 1 in human embryonic stem cell-derived oligodendrocyte precursor cells |
| title_fullStr | Polysialic acid modification of the synaptic cell adhesion molecule SynCAM 1 in human embryonic stem cell-derived oligodendrocyte precursor cells |
| title_full_unstemmed | Polysialic acid modification of the synaptic cell adhesion molecule SynCAM 1 in human embryonic stem cell-derived oligodendrocyte precursor cells |
| title_short | Polysialic acid modification of the synaptic cell adhesion molecule SynCAM 1 in human embryonic stem cell-derived oligodendrocyte precursor cells |
| title_sort | polysialic acid modification of the synaptic cell adhesion molecule syncam 1 in human embryonic stem cell derived oligodendrocyte precursor cells |
| url | http://www.sciencedirect.com/science/article/pii/S1873506115000410 |
| work_keys_str_mv | AT sebastianwerneburg polysialicacidmodificationofthesynapticcelladhesionmoleculesyncam1inhumanembryonicstemcellderivedoligodendrocyteprecursorcells AT falkfrbuettner polysialicacidmodificationofthesynapticcelladhesionmoleculesyncam1inhumanembryonicstemcellderivedoligodendrocyteprecursorcells AT martinamuhlenhoff polysialicacidmodificationofthesynapticcelladhesionmoleculesyncam1inhumanembryonicstemcellderivedoligodendrocyteprecursorcells AT herberthildebrandt polysialicacidmodificationofthesynapticcelladhesionmoleculesyncam1inhumanembryonicstemcellderivedoligodendrocyteprecursorcells |