Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines
Development of an efficient plant regeneration protocol is essential for vegetable crop advancement by biotechnological methods. In this study, regeneration protocols of four pepper lines of different pepper types were optimized. Different protocols for organogenesis were investigated in regeneratio...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2020-02-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3207 |
| _version_ | 1797919360272039936 |
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| author | Tolga İzgü Hülya İlbi Yeşim Yalçın Mendi |
| author_facet | Tolga İzgü Hülya İlbi Yeşim Yalçın Mendi |
| author_sort | Tolga İzgü |
| collection | DOAJ |
| description | Development of an efficient plant regeneration protocol is essential for vegetable crop advancement by biotechnological methods. In this study, regeneration protocols of four pepper lines of different pepper types were optimized. Different protocols for organogenesis were investigated in regeneration experiments. Optimum plant regeneration was obtained in different combinations of 6-Benzylaminopurine (BAP) and Indole-3-acetic acid (IAA) in organogenesis assays. In organogenesis experiment, the highest shoot development was determined as 80% from hypocotyl explant of Demre pepper in 4 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of Charleston pepper in 3 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of capia pepper in 5 mg L-1 BA+0.5 mg L-1 and 84% from hypocotyl explant of bell pepper in 5 mg L-1 BA+0.5 mg L-1 IAA. Afterward, shoots were rooted and whole plants were obtained. |
| first_indexed | 2024-04-10T13:44:18Z |
| format | Article |
| id | doaj.art-3384462226c6438fa27cc785418b4334 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T13:44:18Z |
| publishDate | 2020-02-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-3384462226c6438fa27cc785418b43342023-02-15T16:11:01ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2020-02-018247147710.24925/turjaf.v8i2.471-477.32071539Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) LinesTolga İzgü0Hülya İlbi1Yeşim Yalçın Mendi2Department of Horticulture, Faculty of Agriculture, Ege University, 35040 İzmirDepartment of Horticulture, Faculty of Agriculture, Ege University, 35040 İzmirDepartment of Horticulture, Faculty of Agriculture, Cukurova University, 01330 AdanaDevelopment of an efficient plant regeneration protocol is essential for vegetable crop advancement by biotechnological methods. In this study, regeneration protocols of four pepper lines of different pepper types were optimized. Different protocols for organogenesis were investigated in regeneration experiments. Optimum plant regeneration was obtained in different combinations of 6-Benzylaminopurine (BAP) and Indole-3-acetic acid (IAA) in organogenesis assays. In organogenesis experiment, the highest shoot development was determined as 80% from hypocotyl explant of Demre pepper in 4 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of Charleston pepper in 3 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of capia pepper in 5 mg L-1 BA+0.5 mg L-1 and 84% from hypocotyl explant of bell pepper in 5 mg L-1 BA+0.5 mg L-1 IAA. Afterward, shoots were rooted and whole plants were obtained.http://www.agrifoodscience.com/index.php/TURJAF/article/view/3207tissue cultureorganogenesisplant growth regulatorsshoot formationcallus |
| spellingShingle | Tolga İzgü Hülya İlbi Yeşim Yalçın Mendi Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines Turkish Journal of Agriculture: Food Science and Technology tissue culture organogenesis plant growth regulators shoot formation callus |
| title | Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines |
| title_full | Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines |
| title_fullStr | Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines |
| title_full_unstemmed | Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines |
| title_short | Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines |
| title_sort | optimization of plant regeneration in different pepper capsicum annuum l lines |
| topic | tissue culture organogenesis plant growth regulators shoot formation callus |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3207 |
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