Endoplasmic Reticulum-Shaping Atlastin Proteins Facilitate KSHV Replication
Kaposi’s sarcoma-associated herpesvirus (KSHV) has two life cycle modes: the latent and lytic phases. The endoplasmic reticulum (ER) is the site for KSHV production. Furthermore, ER stress can trigger reactivation of KSHV. Little is known about the nature of the ER factors that regulate KSHV replica...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.790243/full |
| _version_ | 1828133163744886784 |
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| author | Wen-ying Long Guo-hua Zhao Yao Wu |
| author_facet | Wen-ying Long Guo-hua Zhao Yao Wu |
| author_sort | Wen-ying Long |
| collection | DOAJ |
| description | Kaposi’s sarcoma-associated herpesvirus (KSHV) has two life cycle modes: the latent and lytic phases. The endoplasmic reticulum (ER) is the site for KSHV production. Furthermore, ER stress can trigger reactivation of KSHV. Little is known about the nature of the ER factors that regulate KSHV replication. Atlastin proteins (ATLs which include ATL1, ATL2, and ATL3) are large dynamin-related GTPases that control the structure and the dynamics of the ER membrane. Here, we show that ATLs can regulate KSHV lytic activation and infection. Overexpression of ATLs enhances KSHV lytic activation, whereas ATLs silence inhibits it. Intriguingly, we find that silencing of ATLs impairs the response of cells to ER stress, and ER stress can promote the lytic activation of KSHV. Our study establishes that ATLs plays a critically regulatory role in KSHV infection, thus expanding the known scope of biological processes controlled by ATLs to include KSHV infection. |
| first_indexed | 2024-04-11T17:15:35Z |
| format | Article |
| id | doaj.art-3388c1a65c4e4f789488b87f9eb4cd2e |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T17:15:35Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-3388c1a65c4e4f789488b87f9eb4cd2e2022-12-22T04:12:40ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-01-011110.3389/fcimb.2021.790243790243Endoplasmic Reticulum-Shaping Atlastin Proteins Facilitate KSHV ReplicationWen-ying Long0Guo-hua Zhao1Yao Wu2Central Laboratory, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, ChinaNeurology Department, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, ChinaCentral Laboratory, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, ChinaKaposi’s sarcoma-associated herpesvirus (KSHV) has two life cycle modes: the latent and lytic phases. The endoplasmic reticulum (ER) is the site for KSHV production. Furthermore, ER stress can trigger reactivation of KSHV. Little is known about the nature of the ER factors that regulate KSHV replication. Atlastin proteins (ATLs which include ATL1, ATL2, and ATL3) are large dynamin-related GTPases that control the structure and the dynamics of the ER membrane. Here, we show that ATLs can regulate KSHV lytic activation and infection. Overexpression of ATLs enhances KSHV lytic activation, whereas ATLs silence inhibits it. Intriguingly, we find that silencing of ATLs impairs the response of cells to ER stress, and ER stress can promote the lytic activation of KSHV. Our study establishes that ATLs plays a critically regulatory role in KSHV infection, thus expanding the known scope of biological processes controlled by ATLs to include KSHV infection.https://www.frontiersin.org/articles/10.3389/fcimb.2021.790243/fullatlastinendoplasmic reticulumKSHVlytic activationER stress |
| spellingShingle | Wen-ying Long Guo-hua Zhao Yao Wu Endoplasmic Reticulum-Shaping Atlastin Proteins Facilitate KSHV Replication Frontiers in Cellular and Infection Microbiology atlastin endoplasmic reticulum KSHV lytic activation ER stress |
| title | Endoplasmic Reticulum-Shaping Atlastin Proteins Facilitate KSHV Replication |
| title_full | Endoplasmic Reticulum-Shaping Atlastin Proteins Facilitate KSHV Replication |
| title_fullStr | Endoplasmic Reticulum-Shaping Atlastin Proteins Facilitate KSHV Replication |
| title_full_unstemmed | Endoplasmic Reticulum-Shaping Atlastin Proteins Facilitate KSHV Replication |
| title_short | Endoplasmic Reticulum-Shaping Atlastin Proteins Facilitate KSHV Replication |
| title_sort | endoplasmic reticulum shaping atlastin proteins facilitate kshv replication |
| topic | atlastin endoplasmic reticulum KSHV lytic activation ER stress |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.790243/full |
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