SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis
Introduction: Aberrant activation of Semaphorin3C(SEMA3C) is widespread in human cancers. We aimed to analyze SEMA3C expression in cervical cancer and investigate the role of SEMA3C in cervical cancer and its underlying mechanism, which is important for exploring new therapeutic targets and prognost...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2019.01035/full |
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| author | Ruoyan Liu Ruoyan Liu Ruoyan Liu Yanjie Shuai Yanjie Shuai Yanjie Shuai Jingtao Luo Jingtao Luo Jingtao Luo Ze Zhang Ze Zhang Ze Zhang |
| author_facet | Ruoyan Liu Ruoyan Liu Ruoyan Liu Yanjie Shuai Yanjie Shuai Yanjie Shuai Jingtao Luo Jingtao Luo Jingtao Luo Ze Zhang Ze Zhang Ze Zhang |
| author_sort | Ruoyan Liu |
| collection | DOAJ |
| description | Introduction: Aberrant activation of Semaphorin3C(SEMA3C) is widespread in human cancers. We aimed to analyze SEMA3C expression in cervical cancer and investigate the role of SEMA3C in cervical cancer and its underlying mechanism, which is important for exploring new therapeutic targets and prognostic factors.Materials and Methods: The expression of SEMA3C was examined in paraffin-embedded cervical cancer specimens. In vivo and in vitro assays were performed to validate the effect of SEMA3C on cervical cancer cell proliferation and p-ERK pathway activation. Gene Set Enrichment Analysis (GSEA) was performed using The Cancer Genome Atlas (TCGA) data set.Results: SEMA3C expression was associated with poor survival in both the TCGA cohort and our cohort. Silencing of SEMA3C suppressed cervical cancer cell proliferation, colony formation ability, and the activation of the p-ERK signaling pathway in vitro. SEMA3C depletion inhibited tumor growth in vitro. GSEA also showed that the epithelial mesenchymal transition (EMT), TGFβ signaling pathway, angiogenesis, and extracellular matrix (ECM) receptor interactions are associated with a high SEMA3C expression phenotype.Conclusion: SEMA3C is correlated with poor prognosis of cervical cancer patients and promotes tumor growth via the activation of the p-ERK pathway. |
| first_indexed | 2024-12-13T05:42:57Z |
| format | Article |
| id | doaj.art-338aef49b61e4183b768db53dd0bd7b3 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-13T05:42:57Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-338aef49b61e4183b768db53dd0bd7b32022-12-21T23:57:44ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2019-10-01910.3389/fonc.2019.01035478878SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor PrognosisRuoyan Liu0Ruoyan Liu1Ruoyan Liu2Yanjie Shuai3Yanjie Shuai4Yanjie Shuai5Jingtao Luo6Jingtao Luo7Jingtao Luo8Ze Zhang9Ze Zhang10Ze Zhang11Department of Gynecologic Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaTianjin's Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaTianjin's Clinical Research Center for Cancer, Tianjin, ChinaDepartment of Maxillofacial and Otorhinolaryngology Oncology and Department of Head and Neck Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaTianjin's Clinical Research Center for Cancer, Tianjin, ChinaDepartment of Maxillofacial and Otorhinolaryngology Oncology and Department of Head and Neck Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaKey Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaTianjin's Clinical Research Center for Cancer, Tianjin, ChinaDepartment of Maxillofacial and Otorhinolaryngology Oncology and Department of Head and Neck Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, ChinaIntroduction: Aberrant activation of Semaphorin3C(SEMA3C) is widespread in human cancers. We aimed to analyze SEMA3C expression in cervical cancer and investigate the role of SEMA3C in cervical cancer and its underlying mechanism, which is important for exploring new therapeutic targets and prognostic factors.Materials and Methods: The expression of SEMA3C was examined in paraffin-embedded cervical cancer specimens. In vivo and in vitro assays were performed to validate the effect of SEMA3C on cervical cancer cell proliferation and p-ERK pathway activation. Gene Set Enrichment Analysis (GSEA) was performed using The Cancer Genome Atlas (TCGA) data set.Results: SEMA3C expression was associated with poor survival in both the TCGA cohort and our cohort. Silencing of SEMA3C suppressed cervical cancer cell proliferation, colony formation ability, and the activation of the p-ERK signaling pathway in vitro. SEMA3C depletion inhibited tumor growth in vitro. GSEA also showed that the epithelial mesenchymal transition (EMT), TGFβ signaling pathway, angiogenesis, and extracellular matrix (ECM) receptor interactions are associated with a high SEMA3C expression phenotype.Conclusion: SEMA3C is correlated with poor prognosis of cervical cancer patients and promotes tumor growth via the activation of the p-ERK pathway.https://www.frontiersin.org/article/10.3389/fonc.2019.01035/fullcervical cancerSEMA3CGSEATCGAp-ERK signaling pathway |
| spellingShingle | Ruoyan Liu Ruoyan Liu Ruoyan Liu Yanjie Shuai Yanjie Shuai Yanjie Shuai Jingtao Luo Jingtao Luo Jingtao Luo Ze Zhang Ze Zhang Ze Zhang SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis Frontiers in Oncology cervical cancer SEMA3C GSEA TCGA p-ERK signaling pathway |
| title | SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis |
| title_full | SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis |
| title_fullStr | SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis |
| title_full_unstemmed | SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis |
| title_short | SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis |
| title_sort | sema3c promotes cervical cancer growth and is associated with poor prognosis |
| topic | cervical cancer SEMA3C GSEA TCGA p-ERK signaling pathway |
| url | https://www.frontiersin.org/article/10.3389/fonc.2019.01035/full |
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