The Difference of Integrin ανβ3 Expression, Leukemia Inhibitory Factors and Superoxide Dismutase Serum Concentration in the Provision of Kebar Extract (Biophytum petersianum Klotczh), Metformin, and Their Combination to Mouse models of Endometriosis
Background: In endometriosis there is a decrease in endometrial receptivity. Kebar and Metformin grasses are expected to improve the condition of endometrial receptivity Objective: To find out whether there is any effect of giving Kebar grass extract and metformin on endometrial receptivity Metho...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Diponegoro University
2018-07-01
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| Series: | Journal of Biomedicine and Translational Research |
| Subjects: | |
| Online Access: | https://ejournal2.undip.ac.id/index.php/jbtr/article/view/2249 |
| _version_ | 1818197966591098880 |
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| author | Seindy Glamour Syarief Thaufik Hidayat Adi Setyawan Prianto Widjiati Widjiati |
| author_facet | Seindy Glamour Syarief Thaufik Hidayat Adi Setyawan Prianto Widjiati Widjiati |
| author_sort | Seindy Glamour |
| collection | DOAJ |
| description | Background: In endometriosis there is a decrease in endometrial receptivity. Kebar and Metformin grasses are expected to improve the condition of endometrial receptivity
Objective: To find out whether there is any effect of giving Kebar grass extract and metformin on endometrial receptivity
Methods: The study was designed by using a simple experimental randomization. It was conducted in Obstetrics Gynecology Department - Faculty of Medicine Diponegoro University and Faculty of Veterinary Medicine Airlangga University. Sample calculation was employed. The study was done within five groups including control group with normal mice, group of endometriosis mouse models given placebo, group of endometriosis mouse models given Kebar grass extract, group of endometriosis mouse models given metformin, and group of endometriosis mouse models given the combination of Kebar grass – metformin extract. The examination materials of ανβ3 integrin and Leukemia Inhibitory Factors were taken from the uteri of the mice and measured by the Rammele Scale Index (Immuno Reactive Score), while the Superoxide Dismutase examination using ELISA was derived from mice serum. These examinations were performed by two veterinarians
Results: The expression of ανβ3 integrin was significantly higher in the group of mouse models given Kebar grass extract as well as the combination of Kebar and metformin grass extracts (p <0.05) rather than in the group of endometriosis mouse models with no treatment, whereas LIF expression was significantly higher in the group given Kebar grass extract, metformin and combination of both extracts (p <0.05). Serum SOD levels remained the same.
Conclusion: The expressions of ανβ3 Integrin and LIF are higher due to the provision of Kebar grass extract as well as the combination of Kebar grass and metformin extracts |
| first_indexed | 2024-12-12T01:58:22Z |
| format | Article |
| id | doaj.art-338af15656fe456aabd17190792f8f45 |
| institution | Directory Open Access Journal |
| issn | 2503-2178 |
| language | English |
| last_indexed | 2024-12-12T01:58:22Z |
| publishDate | 2018-07-01 |
| publisher | Diponegoro University |
| record_format | Article |
| series | Journal of Biomedicine and Translational Research |
| spelling | doaj.art-338af15656fe456aabd17190792f8f452022-12-22T00:42:16ZengDiponegoro UniversityJournal of Biomedicine and Translational Research2503-21782018-07-01411810.14710/jbtr.v4i1.22491623The Difference of Integrin ανβ3 Expression, Leukemia Inhibitory Factors and Superoxide Dismutase Serum Concentration in the Provision of Kebar Extract (Biophytum petersianum Klotczh), Metformin, and Their Combination to Mouse models of EndometriosisSeindy Glamour0Syarief Thaufik Hidayat1Adi Setyawan Prianto2Widjiati Widjiati3Faculty of Medicine Diponegoro UniversityFaculty of Medicine Diponegoro UniversityFaculty of Medicine Diponegoro UniversityAirlangga University SurabayaBackground: In endometriosis there is a decrease in endometrial receptivity. Kebar and Metformin grasses are expected to improve the condition of endometrial receptivity Objective: To find out whether there is any effect of giving Kebar grass extract and metformin on endometrial receptivity Methods: The study was designed by using a simple experimental randomization. It was conducted in Obstetrics Gynecology Department - Faculty of Medicine Diponegoro University and Faculty of Veterinary Medicine Airlangga University. Sample calculation was employed. The study was done within five groups including control group with normal mice, group of endometriosis mouse models given placebo, group of endometriosis mouse models given Kebar grass extract, group of endometriosis mouse models given metformin, and group of endometriosis mouse models given the combination of Kebar grass – metformin extract. The examination materials of ανβ3 integrin and Leukemia Inhibitory Factors were taken from the uteri of the mice and measured by the Rammele Scale Index (Immuno Reactive Score), while the Superoxide Dismutase examination using ELISA was derived from mice serum. These examinations were performed by two veterinarians Results: The expression of ανβ3 integrin was significantly higher in the group of mouse models given Kebar grass extract as well as the combination of Kebar and metformin grass extracts (p <0.05) rather than in the group of endometriosis mouse models with no treatment, whereas LIF expression was significantly higher in the group given Kebar grass extract, metformin and combination of both extracts (p <0.05). Serum SOD levels remained the same. Conclusion: The expressions of ανβ3 Integrin and LIF are higher due to the provision of Kebar grass extract as well as the combination of Kebar grass and metformin extractshttps://ejournal2.undip.ac.id/index.php/jbtr/article/view/2249Integrin ανβ3Leukemia Inhibitory FactorSuperoxide DismutaseKebar grassMetformin |
| spellingShingle | Seindy Glamour Syarief Thaufik Hidayat Adi Setyawan Prianto Widjiati Widjiati The Difference of Integrin ανβ3 Expression, Leukemia Inhibitory Factors and Superoxide Dismutase Serum Concentration in the Provision of Kebar Extract (Biophytum petersianum Klotczh), Metformin, and Their Combination to Mouse models of Endometriosis Journal of Biomedicine and Translational Research Integrin ανβ3 Leukemia Inhibitory Factor Superoxide Dismutase Kebar grass Metformin |
| title | The Difference of Integrin ανβ3 Expression, Leukemia Inhibitory Factors and Superoxide Dismutase Serum Concentration in the Provision of Kebar Extract (Biophytum petersianum Klotczh), Metformin, and Their Combination to Mouse models of Endometriosis |
| title_full | The Difference of Integrin ανβ3 Expression, Leukemia Inhibitory Factors and Superoxide Dismutase Serum Concentration in the Provision of Kebar Extract (Biophytum petersianum Klotczh), Metformin, and Their Combination to Mouse models of Endometriosis |
| title_fullStr | The Difference of Integrin ανβ3 Expression, Leukemia Inhibitory Factors and Superoxide Dismutase Serum Concentration in the Provision of Kebar Extract (Biophytum petersianum Klotczh), Metformin, and Their Combination to Mouse models of Endometriosis |
| title_full_unstemmed | The Difference of Integrin ανβ3 Expression, Leukemia Inhibitory Factors and Superoxide Dismutase Serum Concentration in the Provision of Kebar Extract (Biophytum petersianum Klotczh), Metformin, and Their Combination to Mouse models of Endometriosis |
| title_short | The Difference of Integrin ανβ3 Expression, Leukemia Inhibitory Factors and Superoxide Dismutase Serum Concentration in the Provision of Kebar Extract (Biophytum petersianum Klotczh), Metformin, and Their Combination to Mouse models of Endometriosis |
| title_sort | difference of integrin ανβ3 expression leukemia inhibitory factors and superoxide dismutase serum concentration in the provision of kebar extract biophytum petersianum klotczh metformin and their combination to mouse models of endometriosis |
| topic | Integrin ανβ3 Leukemia Inhibitory Factor Superoxide Dismutase Kebar grass Metformin |
| url | https://ejournal2.undip.ac.id/index.php/jbtr/article/view/2249 |
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