3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve Regeneration
Abstract Nerve guidance conduits (NGCs) have become a promising alternative for peripheral nerve regeneration; however, the outcome of nerve regeneration and functional recovery is greatly affected by the physical, chemical, and electrical properties of NGCs. In this study, a conductive multiscale f...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-04-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202205744 |
| _version_ | 1797838894209695744 |
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| author | Yongcong Fang Chengjin Wang Zibo Liu Jeonghoon Ko Li Chen Ting Zhang Zhuo Xiong Lei Zhang Wei Sun |
| author_facet | Yongcong Fang Chengjin Wang Zibo Liu Jeonghoon Ko Li Chen Ting Zhang Zhuo Xiong Lei Zhang Wei Sun |
| author_sort | Yongcong Fang |
| collection | DOAJ |
| description | Abstract Nerve guidance conduits (NGCs) have become a promising alternative for peripheral nerve regeneration; however, the outcome of nerve regeneration and functional recovery is greatly affected by the physical, chemical, and electrical properties of NGCs. In this study, a conductive multiscale filled NGC (MF‐NGC) consisting of electrospun poly(lactide‐co‐caprolactone) (PCL)/collagen nanofibers as the sheath, reduced graphene oxide /PCL microfibers as the backbone, and PCL microfibers as the internal structure for peripheral nerve regeneration is developed. The printed MF‐NGCs presented good permeability, mechanical stability, and electrical conductivity, which further promoted the elongation and growth of Schwann cells and neurite outgrowth of PC12 neuronal cells. Animal studies using a rat sciatic nerve injury model reveal that the MF‐NGCs promote neovascularization and M2 transition through the rapid recruitment of vascular cells and macrophages. Histological and functional assessments of the regenerated nerves confirm that the conductive MF‐NGCs significantly enhance peripheral nerve regeneration, as indicated by improved axon myelination, muscle weight increase, and sciatic nerve function index. This study demonstrates the feasibility of using 3D‐printed conductive MF‐NGCs with hierarchically oriented fibers as functional conduits that can significantly enhance peripheral nerve regeneration. |
| first_indexed | 2024-04-09T15:49:23Z |
| format | Article |
| id | doaj.art-338c4b0bb2814707b723019d00627987 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-09T15:49:23Z |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-338c4b0bb2814707b723019d006279872023-04-26T12:15:35ZengWileyAdvanced Science2198-38442023-04-011012n/an/a10.1002/advs.2022057443D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve RegenerationYongcong Fang0Chengjin Wang1Zibo Liu2Jeonghoon Ko3Li Chen4Ting Zhang5Zhuo Xiong6Lei Zhang7Wei Sun8Biomanufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084 P. R. ChinaBiomanufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084 P. R. ChinaBiomanufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084 P. R. ChinaBiomanufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084 P. R. ChinaBiomanufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084 P. R. ChinaBiomanufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084 P. R. ChinaBiomanufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084 P. R. ChinaBiomanufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084 P. R. ChinaBiomanufacturing Center Department of Mechanical Engineering Tsinghua University Beijing 100084 P. R. ChinaAbstract Nerve guidance conduits (NGCs) have become a promising alternative for peripheral nerve regeneration; however, the outcome of nerve regeneration and functional recovery is greatly affected by the physical, chemical, and electrical properties of NGCs. In this study, a conductive multiscale filled NGC (MF‐NGC) consisting of electrospun poly(lactide‐co‐caprolactone) (PCL)/collagen nanofibers as the sheath, reduced graphene oxide /PCL microfibers as the backbone, and PCL microfibers as the internal structure for peripheral nerve regeneration is developed. The printed MF‐NGCs presented good permeability, mechanical stability, and electrical conductivity, which further promoted the elongation and growth of Schwann cells and neurite outgrowth of PC12 neuronal cells. Animal studies using a rat sciatic nerve injury model reveal that the MF‐NGCs promote neovascularization and M2 transition through the rapid recruitment of vascular cells and macrophages. Histological and functional assessments of the regenerated nerves confirm that the conductive MF‐NGCs significantly enhance peripheral nerve regeneration, as indicated by improved axon myelination, muscle weight increase, and sciatic nerve function index. This study demonstrates the feasibility of using 3D‐printed conductive MF‐NGCs with hierarchically oriented fibers as functional conduits that can significantly enhance peripheral nerve regeneration.https://doi.org/10.1002/advs.2022057443D bioprintingaligned microfibergraphene oxidenerve guidance conduitsnerve regeneration |
| spellingShingle | Yongcong Fang Chengjin Wang Zibo Liu Jeonghoon Ko Li Chen Ting Zhang Zhuo Xiong Lei Zhang Wei Sun 3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve Regeneration Advanced Science 3D bioprinting aligned microfiber graphene oxide nerve guidance conduits nerve regeneration |
| title | 3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve Regeneration |
| title_full | 3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve Regeneration |
| title_fullStr | 3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve Regeneration |
| title_full_unstemmed | 3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve Regeneration |
| title_short | 3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve Regeneration |
| title_sort | 3d printed conductive multiscale nerve guidance conduit with hierarchical fibers for peripheral nerve regeneration |
| topic | 3D bioprinting aligned microfiber graphene oxide nerve guidance conduits nerve regeneration |
| url | https://doi.org/10.1002/advs.202205744 |
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