Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway
Abstract Background Epidermal stem cells (EpSCs) play a vital role in wound healing and skin renewal. Although biomaterial scaffolds have been used for transplantation of EpSCs in wound healing, the ex vivo differentiation of EpSCs limits their application. Methods To inhibit the differentiation of...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-021-02418-2 |
| _version_ | 1819100800420413440 |
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| author | Zhixiao Lin Congying Zhao Zhanjun Lei Yuheng Zhang Rong Huang Bin Lin Yuchen Dong Hao Zhang Jinqing Li Xueyong Li |
| author_facet | Zhixiao Lin Congying Zhao Zhanjun Lei Yuheng Zhang Rong Huang Bin Lin Yuchen Dong Hao Zhang Jinqing Li Xueyong Li |
| author_sort | Zhixiao Lin |
| collection | DOAJ |
| description | Abstract Background Epidermal stem cells (EpSCs) play a vital role in wound healing and skin renewal. Although biomaterial scaffolds have been used for transplantation of EpSCs in wound healing, the ex vivo differentiation of EpSCs limits their application. Methods To inhibit the differentiation of EpSCs and maintain their stemness, we developed an electrospun polycaprolactone (PCL)+cellulose acetate (CA) micro/nanofiber for the culture and transplantation of EpSCs. The modulation effect on EpSCs of the scaffold and the underlying mechanism were explored. Liquid chromatography-tandem mass spectrometry for label-free quantitative proteomics was used to analyze proteomic changes in EpSCs cultured on scaffolds. In addition, the role of transplanted undifferentiated EpSCs in wound healing was also studied. Results In this study, we found that the PCL+CA micro/nanofiber scaffold can inhibit the differentiation of EpSCs through YAP activation-mediated inhibition of the Notch signaling pathway. Significantly differentially expressed proteomics was observed in EpSCs cultured on scaffolds and IV collagen-coated culture dishes. Importantly, differential expression levels of ribosome-related proteins and metabolic pathway-related proteins were detected. Moreover, undifferentiated EpSCs transplanted with the PCL+CA scaffold can promote wound healing through the activation of the Notch signaling pathway in rat full-thickness skin defect models. Conclusions Overall, our study demonstrated the role of the PCL+CA micro-nanofiber scaffold in maintaining the stemness of EpSCs for wound healing, which can be helpful for the development of EpSCs maintaining scaffolds and exploration of interactions between biomaterials and EpSCs. |
| first_indexed | 2024-12-22T01:08:32Z |
| format | Article |
| id | doaj.art-338df8680ce641629c2bcff4ed028619 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-22T01:08:32Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-338df8680ce641629c2bcff4ed0286192022-12-21T18:44:02ZengBMCStem Cell Research & Therapy1757-65122021-06-0112111210.1186/s13287-021-02418-2Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathwayZhixiao Lin0Congying Zhao1Zhanjun Lei2Yuheng Zhang3Rong Huang4Bin Lin5Yuchen Dong6Hao Zhang7Jinqing Li8Xueyong Li9Department of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityDepartment of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityDepartment of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityDepartment of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityDepartment of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityDepartment of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityDepartment of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityDepartment of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityDepartment of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityDepartment of Plastic Surgery, Tangdu Hospital, Airforce Military Medical UniversityAbstract Background Epidermal stem cells (EpSCs) play a vital role in wound healing and skin renewal. Although biomaterial scaffolds have been used for transplantation of EpSCs in wound healing, the ex vivo differentiation of EpSCs limits their application. Methods To inhibit the differentiation of EpSCs and maintain their stemness, we developed an electrospun polycaprolactone (PCL)+cellulose acetate (CA) micro/nanofiber for the culture and transplantation of EpSCs. The modulation effect on EpSCs of the scaffold and the underlying mechanism were explored. Liquid chromatography-tandem mass spectrometry for label-free quantitative proteomics was used to analyze proteomic changes in EpSCs cultured on scaffolds. In addition, the role of transplanted undifferentiated EpSCs in wound healing was also studied. Results In this study, we found that the PCL+CA micro/nanofiber scaffold can inhibit the differentiation of EpSCs through YAP activation-mediated inhibition of the Notch signaling pathway. Significantly differentially expressed proteomics was observed in EpSCs cultured on scaffolds and IV collagen-coated culture dishes. Importantly, differential expression levels of ribosome-related proteins and metabolic pathway-related proteins were detected. Moreover, undifferentiated EpSCs transplanted with the PCL+CA scaffold can promote wound healing through the activation of the Notch signaling pathway in rat full-thickness skin defect models. Conclusions Overall, our study demonstrated the role of the PCL+CA micro-nanofiber scaffold in maintaining the stemness of EpSCs for wound healing, which can be helpful for the development of EpSCs maintaining scaffolds and exploration of interactions between biomaterials and EpSCs.https://doi.org/10.1186/s13287-021-02418-2Epidermal stem cellMicro/nanofiber scaffoldCell differentiationWound healingNotch pathway |
| spellingShingle | Zhixiao Lin Congying Zhao Zhanjun Lei Yuheng Zhang Rong Huang Bin Lin Yuchen Dong Hao Zhang Jinqing Li Xueyong Li Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway Stem Cell Research & Therapy Epidermal stem cell Micro/nanofiber scaffold Cell differentiation Wound healing Notch pathway |
| title | Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway |
| title_full | Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway |
| title_fullStr | Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway |
| title_full_unstemmed | Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway |
| title_short | Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway |
| title_sort | epidermal stem cells maintain stemness via a biomimetic micro nanofiber scaffold that promotes wound healing by activating the notch signaling pathway |
| topic | Epidermal stem cell Micro/nanofiber scaffold Cell differentiation Wound healing Notch pathway |
| url | https://doi.org/10.1186/s13287-021-02418-2 |
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