Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials
Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2014-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/03695 |
| _version_ | 1828221971306905600 |
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| author | Kamila Oglęcka Padmini Rangamani Bo Liedberg Rachel S Kraut Atul N Parikh |
| author_facet | Kamila Oglęcka Padmini Rangamani Bo Liedberg Rachel S Kraut Atul N Parikh |
| author_sort | Kamila Oglęcka |
| collection | DOAJ |
| description | Giant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject to a hypotonic bath, giant vesicles consisting of phase separating lipid mixtures undergo osmotic relaxation exhibiting damped oscillations in phase behavior, which is synchronized with swell–burst lytic cycles: in the swelled state, osmotic pressure and elevated membrane tension due to the influx of water promote domain formation. During bursting, solute leakage through transient pores relaxes the pressure and tension, replacing the domain texture by a uniform one. This isothermal phase transition—resulting from a well-coordinated sequence of mechanochemical events—suggests a complex emergent behavior allowing synthetic vesicles produced from simple components, namely, water, osmolytes, and lipids to sense and regulate their micro-environment. |
| first_indexed | 2024-04-12T16:48:45Z |
| format | Article |
| id | doaj.art-338ff7058ff74578a322f56dc0840f8f |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:48:45Z |
| publishDate | 2014-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-338ff7058ff74578a322f56dc0840f8f2022-12-22T03:24:28ZengeLife Sciences Publications LtdeLife2050-084X2014-10-01310.7554/eLife.03695Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentialsKamila Oglęcka0Padmini Rangamani1Bo Liedberg2Rachel S Kraut3Atul N Parikh4Division of Molecular Genetics and Cell Biology, School of Biological Sciences, Nanyang Technological University, Nanyang, Singapore; School of Materials Science and Engineering, Nanyang Technological University, Nanyang, SingaporeDepartment of Molecular and Cellular Biology, University of California, Berkeley, Berkeley, United StatesSchool of Materials Science and Engineering, Nanyang Technological University, Nanyang, SingaporeDivision of Molecular Genetics and Cell Biology, School of Biological Sciences, Nanyang Technological University, Nanyang, SingaporeSchool of Materials Science and Engineering, Nanyang Technological University, Nanyang, Singapore; Department of Biomedical Engineering, University of California, Davis, Davis, United States; Department of Chemical Engineering and Materials Science, University of California, Davis, Davis, United StatesGiant lipid vesicles are closed compartments consisting of semi-permeable shells, which isolate femto- to pico-liter quantities of aqueous core from the bulk. Although water permeates readily across vesicular walls, passive permeation of solutes is hindered. In this study, we show that, when subject to a hypotonic bath, giant vesicles consisting of phase separating lipid mixtures undergo osmotic relaxation exhibiting damped oscillations in phase behavior, which is synchronized with swell–burst lytic cycles: in the swelled state, osmotic pressure and elevated membrane tension due to the influx of water promote domain formation. During bursting, solute leakage through transient pores relaxes the pressure and tension, replacing the domain texture by a uniform one. This isothermal phase transition—resulting from a well-coordinated sequence of mechanochemical events—suggests a complex emergent behavior allowing synthetic vesicles produced from simple components, namely, water, osmolytes, and lipids to sense and regulate their micro-environment.https://elifesciences.org/articles/03695giant phospholipid vesiclelipid raftphase separationprimitive osmoregulationcompartmentalization |
| spellingShingle | Kamila Oglęcka Padmini Rangamani Bo Liedberg Rachel S Kraut Atul N Parikh Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials eLife giant phospholipid vesicle lipid raft phase separation primitive osmoregulation compartmentalization |
| title | Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials |
| title_full | Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials |
| title_fullStr | Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials |
| title_full_unstemmed | Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials |
| title_short | Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials |
| title_sort | oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials |
| topic | giant phospholipid vesicle lipid raft phase separation primitive osmoregulation compartmentalization |
| url | https://elifesciences.org/articles/03695 |
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