Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis
REVEILLE (RVE) genes generally act as core circadian oscillators to regulate multiple developmental events and stress responses in plants. It is of importance to document their roles in crops for utilizing them to improve agronomic traits. Soybean is one of the most important crops worldwide. Howeve...
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| Format: | Article |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.764074/full |
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| author | Binghui Shan Wei Wang Wei Wang Jinfeng Cao Jinfeng Cao Siqi Xia Ruihua Li Shaomin Bian Xuyan Li |
| author_facet | Binghui Shan Wei Wang Wei Wang Jinfeng Cao Jinfeng Cao Siqi Xia Ruihua Li Shaomin Bian Xuyan Li |
| author_sort | Binghui Shan |
| collection | DOAJ |
| description | REVEILLE (RVE) genes generally act as core circadian oscillators to regulate multiple developmental events and stress responses in plants. It is of importance to document their roles in crops for utilizing them to improve agronomic traits. Soybean is one of the most important crops worldwide. However, the knowledge regarding the functional roles of RVEs is extremely limited in soybean. In this study, the soybean gene GmMYB133 was shown to be homologous to the RVE8 clade genes of Arabidopsis. GmMYB133 displayed a non-rhythmical but salt-inducible expression pattern. Like AtRVE8, overexpression of GmMYB133 in Arabidopsis led to developmental defects such as short hypocotyl and late flowering. Seven light-responsive or auxin-associated genes including AtPIF4 were transcriptionally depressed by GmMYB133, suggesting that GmMYB133 might negatively regulate plant growth. Noticeably, the overexpression of GmMYB133 in Arabidopsis promoted seed germination and plant growth under salt stress, and the contents of chlorophylls and malondialdehyde (MDA) were also enhanced and decreased, respectively. Consistently, the expressions of four positive regulators responsive to salt tolerance were remarkably elevated by GmMYB133 overexpression, indicating that GmMYB133 might confer salt stress tolerance. Further observation showed that GmMYB133 overexpression perturbed the clock rhythm of AtPRR5, and yeast one-hybrid assay indicated that GmMYB133 could bind to the AtPRR5 promoter. Moreover, the retrieved ChIP-Seq data showed that AtPRR5 could directly target five clients including AtPIF4. Thus, a regulatory module GmMYB133-PRR5-PIF4 was proposed to regulate plant growth and salt stress tolerance. These findings laid a foundation to further address the functional roles of GmMYB133 and its regulatory mechanisms in soybean. |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T00:03:14Z |
| publishDate | 2021-12-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-3390fb01c1ed40599f13d47b7b7fd36b2022-12-21T18:45:38ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-12-011210.3389/fpls.2021.764074764074Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in ArabidopsisBinghui Shan0Wei Wang1Wei Wang2Jinfeng Cao3Jinfeng Cao4Siqi Xia5Ruihua Li6Shaomin Bian7Xuyan Li8College of Plant Science, Jilin University, Changchun, ChinaHebei Key Laboratory of Crop Salt-Alkali Stress Tolerance Evaluation and Genetic Improvement, Cangzhou, ChinaAcademy of Agricultural and Forestry Sciences, Cangzhou, ChinaHebei Key Laboratory of Crop Salt-Alkali Stress Tolerance Evaluation and Genetic Improvement, Cangzhou, ChinaAcademy of Agricultural and Forestry Sciences, Cangzhou, ChinaCollege of Plant Science, Jilin University, Changchun, ChinaCollege of Plant Science, Jilin University, Changchun, ChinaCollege of Plant Science, Jilin University, Changchun, ChinaCollege of Plant Science, Jilin University, Changchun, ChinaREVEILLE (RVE) genes generally act as core circadian oscillators to regulate multiple developmental events and stress responses in plants. It is of importance to document their roles in crops for utilizing them to improve agronomic traits. Soybean is one of the most important crops worldwide. However, the knowledge regarding the functional roles of RVEs is extremely limited in soybean. In this study, the soybean gene GmMYB133 was shown to be homologous to the RVE8 clade genes of Arabidopsis. GmMYB133 displayed a non-rhythmical but salt-inducible expression pattern. Like AtRVE8, overexpression of GmMYB133 in Arabidopsis led to developmental defects such as short hypocotyl and late flowering. Seven light-responsive or auxin-associated genes including AtPIF4 were transcriptionally depressed by GmMYB133, suggesting that GmMYB133 might negatively regulate plant growth. Noticeably, the overexpression of GmMYB133 in Arabidopsis promoted seed germination and plant growth under salt stress, and the contents of chlorophylls and malondialdehyde (MDA) were also enhanced and decreased, respectively. Consistently, the expressions of four positive regulators responsive to salt tolerance were remarkably elevated by GmMYB133 overexpression, indicating that GmMYB133 might confer salt stress tolerance. Further observation showed that GmMYB133 overexpression perturbed the clock rhythm of AtPRR5, and yeast one-hybrid assay indicated that GmMYB133 could bind to the AtPRR5 promoter. Moreover, the retrieved ChIP-Seq data showed that AtPRR5 could directly target five clients including AtPIF4. Thus, a regulatory module GmMYB133-PRR5-PIF4 was proposed to regulate plant growth and salt stress tolerance. These findings laid a foundation to further address the functional roles of GmMYB133 and its regulatory mechanisms in soybean.https://www.frontiersin.org/articles/10.3389/fpls.2021.764074/fullGmMYB133RVE genesalt tolerancesoybeanhypocotyl elongationPRR5 |
| spellingShingle | Binghui Shan Wei Wang Wei Wang Jinfeng Cao Jinfeng Cao Siqi Xia Ruihua Li Shaomin Bian Xuyan Li Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis Frontiers in Plant Science GmMYB133 RVE gene salt tolerance soybean hypocotyl elongation PRR5 |
| title | Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis |
| title_full | Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis |
| title_fullStr | Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis |
| title_full_unstemmed | Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis |
| title_short | Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis |
| title_sort | soybean gmmyb133 inhibits hypocotyl elongation and confers salt tolerance in arabidopsis |
| topic | GmMYB133 RVE gene salt tolerance soybean hypocotyl elongation PRR5 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.764074/full |
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